PUB7_ARATH
ID PUB7_ARATH Reviewed; 782 AA.
AC Q9CAG5;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 137.
DE RecName: Full=U-box domain-containing protein 7;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 7;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB7 {ECO:0000305};
GN Name=PUB7; OrderedLocusNames=At1g67530; ORFNames=F12B7.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT "The U-box protein family in plants.";
RL Trends Plant Sci. 6:354-358(2001).
RN [6]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC011020; AAG52304.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34659.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34660.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59775.1; -; Genomic_DNA.
DR EMBL; AK117197; BAC41873.1; -; mRNA.
DR EMBL; BT006484; AAP21292.1; -; mRNA.
DR PIR; G96698; G96698.
DR RefSeq; NP_001154455.1; NM_001160983.2.
DR RefSeq; NP_001322110.1; NM_001334315.1.
DR RefSeq; NP_176920.1; NM_105420.3.
DR AlphaFoldDB; Q9CAG5; -.
DR SMR; Q9CAG5; -.
DR BioGRID; 28295; 8.
DR IntAct; Q9CAG5; 3.
DR STRING; 3702.AT1G67530.1; -.
DR iPTMnet; Q9CAG5; -.
DR PaxDb; Q9CAG5; -.
DR PRIDE; Q9CAG5; -.
DR ProteomicsDB; 226262; -.
DR EnsemblPlants; AT1G67530.1; AT1G67530.1; AT1G67530.
DR EnsemblPlants; AT1G67530.2; AT1G67530.2; AT1G67530.
DR EnsemblPlants; AT1G67530.4; AT1G67530.4; AT1G67530.
DR GeneID; 843074; -.
DR Gramene; AT1G67530.1; AT1G67530.1; AT1G67530.
DR Gramene; AT1G67530.2; AT1G67530.2; AT1G67530.
DR Gramene; AT1G67530.4; AT1G67530.4; AT1G67530.
DR KEGG; ath:AT1G67530; -.
DR Araport; AT1G67530; -.
DR TAIR; locus:2008813; AT1G67530.
DR eggNOG; KOG0167; Eukaryota.
DR HOGENOM; CLU_006348_4_1_1; -.
DR InParanoid; Q9CAG5; -.
DR OMA; MYGPYGN; -.
DR OrthoDB; 174560at2759; -.
DR PhylomeDB; Q9CAG5; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9CAG5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CAG5; baseline and differential.
DR Genevisible; Q9CAG5; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 5.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT CHAIN 1..782
FT /note="U-box domain-containing protein 7"
FT /id="PRO_0000322152"
FT DOMAIN 271..345
FT /note="U-box"
FT REPEAT 456..499
FT /note="ARM 1"
FT REPEAT 502..541
FT /note="ARM 2"
FT REPEAT 542..581
FT /note="ARM 3"
FT REPEAT 583..623
FT /note="ARM 4"
FT REPEAT 626..665
FT /note="ARM 5"
FT REGION 707..765
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 707..727
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 735..754
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 782 AA; 86343 MW; 53C31939E0DB0292 CRC64;
MDVTELEENL FAASDAKLHG DMCKELSGVL CKVLSIFPSL EGARPRSKSG IQALCSLHIA
LEKAKNILQH CSECSKLYLA ITGDAVLLKF EKAKIALIDG LKRVEDIVPS SIGSQILEIV
GELENTRFML DPSEKEVGDQ IIALLQQGKK FDNCNDNTEL EIFHRAATRL SITSSRVALA
ERRALKKLID RARAEEDKRK ESIVAYLLHL MRKCSKLFRS EILDENDSPG SYPCSPNEDH
GSVHGFGRQL SRFGSLNDKP MNSINSGQMP VPPEELRCPI SLQLMCDPVI IASGQTYERV
CIEKWFSDGH NTCPKTQQQL PHISLTPNNC VKGLIASWCE QNGTQIPSGP PESQDLDYWR
LALSDSESTK SQSVNSIGSY KLKGVKIVPL EENGTTVVER QNTEESFVSD DDDEEDSDIN
VLERYQDLLA VLNEEEGLEK KCKVVEKIRL LLKDDEEARI FMGANGFVEA LLRFLGSAVD
DNNAAAQDSG AMALFNLAVN NNRNKELMLT SGVIRLLEKM ISSAESHGSA TALYLNLSCL
DEAKSVIGSS QAVPFLVQLL QKEIETQCKL DALHALYNLS TYSPNIPALL SSNIIKSLQG
LLASTGENLW IEKSLAVLLN LASSQEGKDE AVSSQGMISS LATVLDMGDT TEQEQAVSCL
LILCNGRESC IQMVLQEGVI PSLVSISVNG TPRGREKSQK LLMLFREERQ QRDQPSSNRD
EPPQKEPARK SLSAPLSVHG STPASASVQD YEPRVLSKSM SRRKSMARPF SFFWKKSYSV
RE
