PUF6_CAEEL
ID PUF6_CAEEL Reviewed; 485 AA.
AC O01322;
DT 13-NOV-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Pumilio domain-containing protein 6 {ECO:0000303|PubMed:17234175};
GN Name=puf-6 {ECO:0000312|EMBL:CAB04133.1, ECO:0000312|WormBase:F18A11.1};
GN ORFNames=F18A11.1;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17234175; DOI=10.1016/j.ydbio.2006.12.004;
RA Lublin A.L., Evans T.C.;
RT "The RNA-binding proteins PUF-5, PUF-6, and PUF-7 reveal multiple systems
RT for maternal mRNA regulation during C. elegans oogenesis.";
RL Dev. Biol. 303:635-649(2007).
RN [2] {ECO:0000312|EMBL:CAB04133.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:CAB04133.1};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP DISRUPTION PHENOTYPE.
RX PubMed=10518502; DOI=10.1242/dev.126.21.4861;
RA Subramaniam K., Seydoux G.;
RT "nos-1 and nos-2, two genes related to Drosophila nanos, regulate
RT primordial germ cell development and survival in Caenorhabditis elegans.";
RL Development 126:4861-4871(1999).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=18579869; DOI=10.1261/rna.1095908;
RA Stumpf C.R., Kimble J., Wickens M.;
RT "A Caenorhabditis elegans PUF protein family with distinct RNA binding
RT specificity.";
RL RNA 14:1550-1557(2008).
RN [5] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22542599; DOI=10.1016/j.ydbio.2012.03.019;
RA Hubstenberger A., Cameron C., Shtofman R., Gutman S., Evans T.C.;
RT "A network of PUF proteins and Ras signaling promote mRNA repression and
RT oogenesis in C. elegans.";
RL Dev. Biol. 366:218-231(2012).
CC -!- FUNCTION: RNA-binding protein that binds to the consensus sequence 5'-
CC CUCUGUAUCUUGU-3' in mRNA 3'-UTRs and modulates mRNA expression and
CC stability. Functions redundantly with puf-5 and puf-7 in oocyte
CC formation and organization, early embryonic cell divisions, and
CC repression of expression of glp-1 and other maternal mRNAs in late
CC oogenesis. {ECO:0000269|PubMed:17234175, ECO:0000269|PubMed:18579869,
CC ECO:0000269|PubMed:22542599}.
CC -!- DISRUPTION PHENOTYPE: Simultaneous knockdown of puf-6 and puf-7 results
CC in ectopic primordial germ cells (PGCs) outside the somatic gonads,
CC premature PGC proliferation in L1 larvae and germ cell death. Does not
CC affect germline development in adult hermaphrodites. Disruption with
CC puf-5 and puf-7 results in abnormally small oocytes, disorganization of
CC oocyte nuclei and cells, inefficient yolk uptake by oocytes, embryonic
CC arrest with impaired eggshell formation and cytokinesis defects,
CC impaired repression of glp-1 in late oogenesis, and mislocalization of
CC rme-2 to the cytoplasm instead of the plasma membrane.
CC {ECO:0000269|PubMed:10518502, ECO:0000269|PubMed:17234175,
CC ECO:0000269|PubMed:22542599}.
CC -!- MISCELLANEOUS: Puf-6 and puf-7 are >98% identical on both nucleotide
CC and protein sequence level. Experimental approaches often do not
CC distinguish between the two genes, which are collectively referred to
CC as puf6/7 and are considered to be functionally redundant.
CC {ECO:0000305}.
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DR EMBL; Z81507; CAB04133.1; -; Genomic_DNA.
DR PIR; T21080; T21080.
DR RefSeq; NP_496773.1; NM_064372.6.
DR AlphaFoldDB; O01322; -.
DR SMR; O01322; -.
DR BioGRID; 40245; 2.
DR STRING; 6239.F18A11.1.1; -.
DR PaxDb; O01322; -.
DR PeptideAtlas; O01322; -.
DR EnsemblMetazoa; F18A11.1.1; F18A11.1.1; WBGene00004242.
DR GeneID; 174947; -.
DR KEGG; cel:CELE_F18A11.1; -.
DR UCSC; F18A11.1.1; c. elegans.
DR CTD; 174947; -.
DR WormBase; F18A11.1; CE09451; WBGene00004242; puf-6.
DR eggNOG; KOG1488; Eukaryota.
DR GeneTree; ENSGT00970000196107; -.
DR HOGENOM; CLU_028494_0_0_1; -.
DR InParanoid; O01322; -.
DR OMA; NGRIARC; -.
DR OrthoDB; 238378at2759; -.
DR PhylomeDB; O01322; -.
DR PRO; PR:O01322; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00004242; Expressed in adult organism and 2 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:WormBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; ISS:WormBase.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007281; P:germ cell development; IMP:WormBase.
DR GO; GO:0006402; P:mRNA catabolic process; ISS:WormBase.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IMP:WormBase.
DR GO; GO:0017148; P:negative regulation of translation; ISS:WormBase.
DR GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR Pfam; PF00806; PUF; 7.
DR SMART; SM00025; Pumilio; 7.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 8.
DR PROSITE; PS50303; PUM_HD; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Developmental protein; Differentiation;
KW Oogenesis; Reference proteome; Repeat; RNA-binding; Translation regulation.
FT CHAIN 1..485
FT /note="Pumilio domain-containing protein 6"
FT /id="PRO_0000424281"
FT REPEAT 86..124
FT /note="Pumilio 1"
FT /evidence="ECO:0000255"
FT REPEAT 125..163
FT /note="Pumilio 2"
FT /evidence="ECO:0000255"
FT REPEAT 164..200
FT /note="Pumilio 3"
FT /evidence="ECO:0000255"
FT REPEAT 201..236
FT /note="Pumilio 4"
FT /evidence="ECO:0000255"
FT REPEAT 237..279
FT /note="Pumilio 5"
FT /evidence="ECO:0000255"
FT REPEAT 287..324
FT /note="Pumilio 6"
FT /evidence="ECO:0000255"
FT REPEAT 326..361
FT /note="Pumilio 7"
FT /evidence="ECO:0000255"
FT REPEAT 372..411
FT /note="Pumilio 8"
FT /evidence="ECO:0000255"
FT REGION 29..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 55..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 439..454
FT /note="RNA-binding"
FT /evidence="ECO:0000250|UniProtKB:O44169"
FT COMPBIAS 55..71
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 485 AA; 54889 MW; 482F183C5DBAA1E9 CRC64;
MTPNRRSTDS YNMLGASFDF DPDFSLLSNK THKNKNPKPP VKLLPYRHGS NTTSSDLDNY
IFNSGSGSSD DETPPPAAPI FISLEEVLLN GLLIDFAIDP SGVKFLEANY PLDSEDQIRK
AVFEKLTEST TLFVGLCHSR NGNFIVQKLV ELATPAEQRE LLRQMIDGGL LVMCKDKFAC
RVVQLALQKF DHSNVFQLIQ ELSTFDLAAM CTDQISIHVI QRVVKQLPVD MWTFFVHFLS
SGDSLMAVCQ DKYGCRLVQQ VIDRLAENPK LPCFKFRIQL LHSLMTCIVR NCYRLSSNEF
ANYVIQYVIK SSGIMEMYRD TIIDKCLLRN LLSMSQDKYA SHVIEGAFLF APPALLHEMM
EEIFSGYVKD VELNRDALDI LLFHQYGNYV VQQMISICTA ALIGKEERQL PPAILLLYSG
WYEKMKQRVL QHASRLERFS SGKKIIDSVM RHGVPTAAAI NAQAAPSLME LTAQFDAMFP
SFLAR
