PUF6_SCHPO
ID PUF6_SCHPO Reviewed; 642 AA.
AC Q9UU76;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Pumilio homology domain family member 6;
GN Name=puf6; ORFNames=SPCP1E11.11;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34; SER-36; SER-79; SER-81
RP AND SER-88, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: RNA-binding protein involved in post-transcriptional
CC regulation. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the PUF6 family. {ECO:0000305}.
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DR EMBL; CU329672; CAB54870.1; -; Genomic_DNA.
DR PIR; T41690; T41690.
DR RefSeq; NP_588564.1; NM_001023551.2.
DR AlphaFoldDB; Q9UU76; -.
DR SMR; Q9UU76; -.
DR BioGRID; 275414; 21.
DR STRING; 4896.SPCP1E11.11.1; -.
DR iPTMnet; Q9UU76; -.
DR MaxQB; Q9UU76; -.
DR PaxDb; Q9UU76; -.
DR PRIDE; Q9UU76; -.
DR EnsemblFungi; SPCP1E11.11.1; SPCP1E11.11.1:pep; SPCP1E11.11.
DR GeneID; 2538833; -.
DR KEGG; spo:SPCP1E11.11; -.
DR PomBase; SPCP1E11.11; puf6.
DR VEuPathDB; FungiDB:SPCP1E11.11; -.
DR eggNOG; KOG2050; Eukaryota.
DR HOGENOM; CLU_013994_1_0_1; -.
DR InParanoid; Q9UU76; -.
DR OMA; WILDDVY; -.
DR PhylomeDB; Q9UU76; -.
DR PRO; PR:Q9UU76; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005730; C:nucleolus; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; ISO:PomBase.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0000900; F:mRNA regulatory element binding translation repressor activity; ISO:PomBase.
DR GO; GO:0006417; P:regulation of translation; IBA:GO_Central.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; ISO:PomBase.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR012959; CPL_dom.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR040059; PUM3.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR PANTHER; PTHR13389; PTHR13389; 1.
DR Pfam; PF08144; CPL; 1.
DR SMART; SM00025; Pumilio; 5.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 6.
DR PROSITE; PS50303; PUM_HD; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor;
KW RNA-binding; Translation regulation.
FT CHAIN 1..642
FT /note="Pumilio homology domain family member 6"
FT /id="PRO_0000362160"
FT DOMAIN 139..489
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 160..196
FT /note="Pumilio 1"
FT REPEAT 197..232
FT /note="Pumilio 2"
FT REPEAT 233..269
FT /note="Pumilio 3"
FT REPEAT 345..381
FT /note="Pumilio 4"
FT REPEAT 382..418
FT /note="Pumilio 5"
FT REPEAT 420..460
FT /note="Pumilio 6"
FT REGION 1..124
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..38
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 39..53
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 54..68
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 69..95
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 96..124
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 34
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 36
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 79
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 81
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 88
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 642 AA; 72635 MW; 1D2D6161F3A790EF CRC64;
MAIGKRKEST EVKKKDGSMN KRVKVAKLPK KVDSFSPKKK KNTTSSGSSE SDSMSQNDKK
KDSSLNESED EDFAGFGESA SENDELESAE SEAENDEESS SQKSNSKESH AQRKKLQKER
KAMKPFADTS LKAKSLWDKL RQKTSIKAEE RKTIIAELFD LIRTNVKQLV FKHDMSRVVQ
TCVKFGSKQQ RETICAELAG SYVDLCKSPY GKYLAIKIFK YGTPKMKEVI LGEMYGNVVK
MIRHREAAYV VEDAFREFTN LQQQRALICE FYGPEFQVFK DRTQDIHIDK LLIDHPEKRP
SIMQNLWKTI EGSIAKGSIG FTMVHRAMLE FINHADSNEA KELLNLTKEL IYEFVHTRDG
SQVAMKLFAL ANAKDRKVML KSLRPYLIET AKDSYGHLVV VAALDCTDDT IMTGKLLQAE
FEGELLKLSA DKFARRILLY VLVGWEDARY FSKENRELLR SLDSLKAKTS KKDPIVRRNE
LKATIGPLLI SLISKAAGDM IAESLASQVL VDALLYAPCE KEEAVDATLK AFDGNPEQDN
HLIHQIHCSR ALKTLVQNGH WSGAEKQVVK AEDDLKVASK LIVIIKKYLV EWASGDGAFV
VVAVLEALSD SEKQEFLKIL RKHKNQLNKS EFRGTKKLLE ML
