PUG1_YEAST
ID PUG1_YEAST Reviewed; 303 AA.
AC P40100; D3DM94;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Protoporphyrin uptake protein 1;
GN Name=PUG1; OrderedLocusNames=YER185W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP PROBABLE TOPOLOGY.
RX PubMed=12524434; DOI=10.1074/jbc.m300163200;
RA Kim H., Melen K., von Heijne G.;
RT "Topology models for 37 Saccharomyces cerevisiae membrane proteins based on
RT C-terminal reporter fusions and predictions.";
RL J. Biol. Chem. 278:10208-10213(2003).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=18326586; DOI=10.1128/ec.00414-07;
RA Protchenko O., Shakoury-Elizeh M., Keane P., Storey J., Androphy R.,
RA Philpott C.C.;
RT "Role of PUG1 in inducible porphyrin and heme transport in Saccharomyces
RT cerevisiae.";
RL Eukaryot. Cell 7:859-871(2008).
RN [5]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS].
RX PubMed=19756047; DOI=10.1038/msb.2009.64;
RA Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.;
RT "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals
RT new roles for protein glycosylation in eukaryotes.";
RL Mol. Syst. Biol. 5:308-308(2009).
CC -!- FUNCTION: Involved in inducible protoporphyrin IX influx and heme
CC efflux. {ECO:0000269|PubMed:18326586}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: By oxygen and heme deficiency.
CC {ECO:0000269|PubMed:18326586}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:19756047}.
CC -!- SIMILARITY: Belongs to the lipid-translocating exporter (LTE) (TC
CC 9.A.26.1) family. {ECO:0000305}.
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DR EMBL; U18922; AAB64712.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07848.1; -; Genomic_DNA.
DR PIR; S50688; S50688.
DR RefSeq; NP_011112.3; NM_001179075.3.
DR AlphaFoldDB; P40100; -.
DR BioGRID; 36939; 43.
DR IntAct; P40100; 1.
DR STRING; 4932.YER185W; -.
DR TCDB; 9.A.26.1.3; the lipid-translocating exporter (lte) family.
DR iPTMnet; P40100; -.
DR PaxDb; P40100; -.
DR EnsemblFungi; YER185W_mRNA; YER185W; YER185W.
DR GeneID; 856934; -.
DR KEGG; sce:YER185W; -.
DR SGD; S000000987; PUG1.
DR VEuPathDB; FungiDB:YER185W; -.
DR eggNOG; ENOG502QURG; Eukaryota.
DR GeneTree; ENSGT00940000176387; -.
DR HOGENOM; CLU_033465_3_1_1; -.
DR InParanoid; P40100; -.
DR OMA; WFMIPFA; -.
DR BioCyc; YEAST:G3O-30341-MON; -.
DR PRO; PR:P40100; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P40100; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0035351; P:heme transmembrane transport; IMP:SGD.
DR InterPro; IPR007568; RTA1.
DR PANTHER; PTHR31465; PTHR31465; 1.
DR Pfam; PF04479; RTA1; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..303
FT /note="Protoporphyrin uptake protein 1"
FT /id="PRO_0000202661"
FT TOPO_DOM 1..18
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 40..76
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 98..111
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 133..154
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 176..183
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 205..226
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 248..264
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 286..303
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 303 AA; 33733 MW; 0E348C4A7CE0A772 CRC64;
MSTTDSGFVL YHYTPSKAAA IVFVVLFIIM TVIFAVQTLY AARKSSKALK NNPFESSDDK
VDSLEDAEYK QLKITPTVFA FIPFFTGCIM EAVGYIGRAL SSSNPERTTP YIIQSVLLLV
APALIAATIY MIFGRLLHVM RCQSLILISA RFGTTFFVVG DVFSFFLQAA GGGLMSKAGS
TKTGSGLITA GLFVQVIFFG FFIINEIRFT VNVKRRCLFY EDISRKWIFV NATLLLSSML
ILLRSIVRIV EFIQGFNGYI ISHEYFIYVF DAVPMLLVII AFSVGSFFGN VFDVIKECQT
LSN
