ID   PUL1_KLULA              Reviewed;         480 AA.
AC   Q6CSN3;
DT   05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Probable cyclodipeptide synthase PUL1 {ECO:0000303|PubMed:30297402};
DE            Short=CDPS PUL1 {ECO:0000303|PubMed:30297402};
DE            EC=6.-.-.- {ECO:0000305|PubMed:30297402};
DE   AltName: Full=Pulcherrimin biosynthesis cluster protein 1 {ECO:0000303|PubMed:30297402};
GN   Name=PUL1 {ECO:0000303|PubMed:30297402}; OrderedLocusNames=KLLA0_C19184g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
RN   [2]
RP   IDENTIFICATION, DISRUPTION PHENOTYPE, FUNCTION, AND PATHWAY.
RX   PubMed=30297402; DOI=10.1073/pnas.1806268115;
RA   Krause D.J., Kominek J., Opulente D.A., Shen X.X., Zhou X., Langdon Q.K.,
RA   DeVirgilio J., Hulfachor A.B., Kurtzman C.P., Rokas A., Hittinger C.T.;
RT   "Functional and evolutionary characterization of a secondary metabolite
RT   gene cluster in budding yeasts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 115:11030-11035(2018).
CC   -!- FUNCTION: Probable cyclodipeptide synthase; part of the PUL gene
CC       cluster that mediates the formation of pulcherrimin, a red iron-
CC       containing pigment composed of two cyclized and modified leucine
CC       molecules that acts as a siderophore, a chelator that binds iron
CC       outside the cell for subsequent uptake (PubMed:30297402). Two leucine
CC       molecules are cyclized via a cyclodipeptide synthase, and the resulting
CC       diketopiperazine is oxidized by a cytochrome P450 monooxygenase to
CC       generate pulcherriminic acid (PA), which can then spontaneously bind
CC       iron to form pulcherrimin (PubMed:30297402). The probable
CC       cyclodipeptide synthase PUL1 and the cytochrome P450 monooxygenase PUL2
CC       encode the enzymes responsible for the two-step pulcherrimin
CC       biosynthesis pathway (Probable). {ECO:0000269|PubMed:30297402,
CC       ECO:0000305|PubMed:30297402}.
CC   -!- PATHWAY: Siderophore biosynthesis. {ECO:0000269|PubMed:30297402}.
CC   -!- DISRUPTION PHENOTYPE: Impairs pulcherrimin production and subsequent
CC       red pigmentation. {ECO:0000269|PubMed:30297402}.
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DR   EMBL; CR382123; CAH01907.1; -; Genomic_DNA.
DR   RefSeq; XP_453056.1; XM_453056.1.
DR   AlphaFoldDB; Q6CSN3; -.
DR   EnsemblFungi; CAH01907; CAH01907; KLLA0_C19184g.
DR   GeneID; 2892648; -.
DR   KEGG; kla:KLLA0_C19184g; -.
DR   HOGENOM; CLU_568653_0_0_1; -.
DR   InParanoid; Q6CSN3; -.
DR   OMA; GCHLRAQ; -.
DR   Proteomes; UP000000598; Chromosome C.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
PE   4: Predicted;
KW   Ligase; Reference proteome.
FT   CHAIN           1..480
FT                   /note="Probable cyclodipeptide synthase PUL1"
FT                   /id="PRO_0000445898"
SQ   SEQUENCE   480 AA;  55417 MW;  5D00FEBFE99CF91E CRC64;
     MYQLLFQRLG VTLTAGNDKK TSIPSNQLVG HLIGLILLCD DLNEAFADFQ ALLQNGIAIS
     SSDRGYLVFD AHVTDCGCHL RAQMIQDVFS YFKNHEVTKL YIFDAVAKKL NELKRHCISL
     IQTLCWENTS AQKLGFPKNI KSYEELLKLL QWDTADISSA IADSFPMNPN NADQNEKGLV
     WNVTKLEVVL EFLYCSHFLS KNKIYNKKEN LDSVTIDFNN AFEKRQLLSN KFHCQGTGKL
     GVGCRYLKHA KQSKNSFISV VSNLQSRLAL LSIAFLKSRC TLPCDIEALQ KNSPRNVSAI
     PNFLHFLILE REWSQNETPI LLAVRKLHEH EHCDLYFEAR INPHTFEWTL QHKECCEFEH
     HTPYIVITAL ATGSSTTKTA QLLAWELMKA QKNFRQFWLT FMSQHRQYPF EIEHDEDQLL
     ETQVSQDIFE LYCQSKREDR NQILFDDSTS LLPKHIFTEY PSIFFNFQKN VCSKHGALVI