ID   PUL4_KLULA              Reviewed;         591 AA.
AC   Q6CSN1;
DT   05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Transcriptional regulator PUL4 {ECO:0000303|PubMed:30297402};
DE   AltName: Full=Pulcherrimin biosynthesis cluster protein 4 {ECO:0000303|PubMed:30297402};
GN   Name=PUL4 {ECO:0000303|PubMed:30297402}; OrderedLocusNames=KLLA0_C19228g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
RN   [2]
RP   IDENTIFICATION, DISRUPTION PHENOTYPE, AND FUNCTION.
RX   PubMed=30297402; DOI=10.1073/pnas.1806268115;
RA   Krause D.J., Kominek J., Opulente D.A., Shen X.X., Zhou X., Langdon Q.K.,
RA   DeVirgilio J., Hulfachor A.B., Kurtzman C.P., Rokas A., Hittinger C.T.;
RT   "Functional and evolutionary characterization of a secondary metabolite
RT   gene cluster in budding yeasts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 115:11030-11035(2018).
CC   -!- FUNCTION: Transcription factor involved in regulation of the PUL gene
CC       cluster that mediates the formation of pulcherrimin, a red iron-
CC       containing pigment composed of two cyclized and modified leucine
CC       molecules that acts as a siderophore, a chelator that binds iron
CC       outside the cell for subsequent uptake. {ECO:0000269|PubMed:30297402}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- DISRUPTION PHENOTYPE: Impairs pulcherrimin production and subsequent
CC       red pigmentation. {ECO:0000269|PubMed:30297402}.
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DR   EMBL; CR382123; CAH01909.1; -; Genomic_DNA.
DR   RefSeq; XP_453058.1; XM_453058.1.
DR   AlphaFoldDB; Q6CSN1; -.
DR   SMR; Q6CSN1; -.
DR   STRING; 28985.XP_453058.1; -.
DR   EnsemblFungi; CAH01909; CAH01909; KLLA0_C19228g.
DR   GeneID; 2892646; -.
DR   KEGG; kla:KLLA0_C19228g; -.
DR   eggNOG; ENOG502QRWI; Eukaryota.
DR   HOGENOM; CLU_449939_0_0_1; -.
DR   InParanoid; Q6CSN1; -.
DR   OMA; LMANFMT; -.
DR   Proteomes; UP000000598; Chromosome C.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..591
FT                   /note="Transcriptional regulator PUL4"
FT                   /id="PRO_0000445900"
FT   DNA_BIND        3..29
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
SQ   SEQUENCE   591 AA;  67427 MW;  D4B48A11732BF5CE CRC64;
     MACLECKKRK QKCDGQKPCR RCTKLNVKCI YGTDRRKDKR KIKDGSNMFI FKNQTLCNDK
     INGIVPHPLS HDTITTKETW EPSYPLFSDD INPADIISME NTDGSIPLQF DLDFTSLESC
     DVNDFLRLIG DTFPANDADT LDMNQMGGFN TPSISHNTQD DKSQIAIQRN RLIDVIFGDD
     SHTPPGILRE HIFELSERHE DLEALDFDDN GKFLLSTVLC LGALTLRKRE LLNRDSNQPS
     TNGIPEVAAG AYKYYTIATD LIPAVHAAPN IDGFCGLVLM ANFMTIMIPL EGQLYLSNNA
     LEVAVALNFH KRESYDEMIV SNPAQLGVFL LFWNLWCSSC MLATLLGKQP FLTLDNISLP
     PPHQMQHTVS SSPLSINFMR LRIQLATLQT KIFQRLYVYG SLNKVLFQEI ETELSLLSTQ
     ISNMKCYPIY DEGLFYRSKV LMLELSCLKA HNAFLLYRPN LIQKKSLHAV DAAKHIILEI
     WSHYTKQFPK NEKDLVDHLD WNFSYPLRTA SLTLSISCVI LQKYQQSLNF LEEYGIFEYN
     LALGVLNDLI QVVPIEKRLI NLLTVSRTTV EDANESNRED SLRFWTNMLM C