PULA_KLEPN
ID PULA_KLEPN Reviewed; 1090 AA.
AC P07206;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 2.
DT 25-MAY-2022, entry version 146.
DE RecName: Full=Pullulanase;
DE EC=3.2.1.41;
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase;
DE AltName: Full=Pullulan 6-glucanohydrolase;
DE Flags: Precursor;
GN Name=pulA;
OS Klebsiella pneumoniae.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=573;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=UNF 5023;
RX PubMed=2181242; DOI=10.1111/j.1365-2958.1990.tb02016.x;
RA Kornacker M.G., Pugsley A.P.;
RT "Molecular characterization of pulA and its product, pullulanase, a
RT secreted enzyme of Klebsiella pneumoniae UNF5023.";
RL Mol. Microbiol. 4:73-85(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-62.
RX PubMed=3902792; DOI=10.1128/jb.164.2.639-645.1985;
RA Chapon C., Raibaud O.;
RT "Structure of two divergent promoters located in front of the gene encoding
RT pullulanase in Klebsiella pneumoniae and positively regulated by the malT
RT product.";
RL J. Bacteriol. 164:639-645(1985).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 944-1090.
RX PubMed=2661532; DOI=10.1128/jb.171.7.3673-3679.1989;
RA D'Enfert C., Pugsley A.P.;
RT "Klebsiella pneumoniae pulS gene encodes an outer membrane lipoprotein
RT required for pullulanase secretion.";
RL J. Bacteriol. 171:3673-3679(1989).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC -!- SUBUNIT: Homotrimer.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. {ECO:0000305}.
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DR EMBL; X52181; CAA36431.1; -; Genomic_DNA.
DR EMBL; M12503; AAA25087.2; -; Genomic_DNA.
DR EMBL; M29097; AAA61976.1; -; Genomic_DNA.
DR PIR; S11823; S11823.
DR PIR; S38801; S38801.
DR PDB; 2YOC; X-ray; 2.88 A; A/B=21-1089.
DR PDBsum; 2YOC; -.
DR AlphaFoldDB; P07206; -.
DR SMR; P07206; -.
DR CAZy; CBM41; Carbohydrate-Binding Module Family 41.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR BRENDA; 3.2.1.41; 2814.
DR EvolutionaryTrace; P07206; -.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0051060; F:pullulanase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd10315; CBM41_pullulanase; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 2.60.40.1180; -; 1.
DR InterPro; IPR013784; Carb-bd-like_fold.
DR InterPro; IPR005323; CBM41_pullulanase.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR011839; Pullul_strch.
DR InterPro; IPR024561; Pullul_strch_C.
DR InterPro; IPR041111; Pullulanase_Ins.
DR InterPro; IPR040671; Pullulanase_N2.
DR InterPro; IPR045041; Pullulanase_PULA-like.
DR PANTHER; PTHR43631; PTHR43631; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF11852; DUF3372; 1.
DR Pfam; PF03714; PUD; 1.
DR Pfam; PF18494; Pullulanase_Ins; 1.
DR Pfam; PF17967; Pullulanase_N2; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF49452; SSF49452; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF81296; SSF81296; 2.
DR TIGRFAMs; TIGR02103; pullul_strch; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Glycosidase; Hydrolase; Lipoprotein; Membrane;
KW Palmitate; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 20..1090
FT /note="Pullulanase"
FT /id="PRO_0000001427"
FT ACT_SITE 684
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 713
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT SITE 841
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250"
FT LIPID 20
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 20
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CONFLICT 6
FT /note="R -> C (in Ref. 2; AAA25087)"
FT /evidence="ECO:0000305"
FT CONFLICT 10
FT /note="V -> F (in Ref. 2; AAA25087)"
FT /evidence="ECO:0000305"
FT CONFLICT 15
FT /note="V -> I (in Ref. 2; AAA25087)"
FT /evidence="ECO:0000305"
FT CONFLICT 23
FT /note="G -> S (in Ref. 2; AAA25087)"
FT /evidence="ECO:0000305"
FT CONFLICT 31
FT /note="N -> S (in Ref. 2; AAA25087)"
FT /evidence="ECO:0000305"
FT CONFLICT 34
FT /note="T -> N (in Ref. 2; AAA25087)"
FT /evidence="ECO:0000305"
FT CONFLICT 36
FT /note="D -> DGNP (in Ref. 2; AAA25087)"
FT /evidence="ECO:0000305"
FT CONFLICT 55..58
FT /note="TAVE -> MATA (in Ref. 2; AAA25087)"
FT /evidence="ECO:0000305"
FT STRAND 60..66
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 68..71
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 80..87
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 95..97
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 118..124
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 126..144
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 146..149
FT /evidence="ECO:0007829|PDB:2YOC"
FT TURN 150..152
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 157..161
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 168..170
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 171..178
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 186..188
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 190..195
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 197..199
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 203..213
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 221..223
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 231..233
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 237..242
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 244..246
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 251..253
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 260..263
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 266..273
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 278..286
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 288..299
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 305..309
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 312..318
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 323..330
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 336..341
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 343..345
FT /evidence="ECO:0007829|PDB:2YOC"
FT TURN 346..349
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 350..355
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 357..359
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 363..371
FT /evidence="ECO:0007829|PDB:2YOC"
FT TURN 373..375
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 377..383
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 389..391
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 393..395
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 397..399
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 406..408
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 425..429
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 432..436
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 438..442
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 450..452
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 456..460
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 465..476
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 480..484
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 487..492
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 496..498
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 506..512
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 514..517
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 522..525
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 526..528
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 531..539
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 548..556
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 559..561
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 568..574
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 586..600
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 605..610
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 613..616
FT /evidence="ECO:0007829|PDB:2YOC"
FT TURN 619..621
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 627..630
FT /evidence="ECO:0007829|PDB:2YOC"
FT TURN 632..634
FT /evidence="ECO:0007829|PDB:2YOC"
FT TURN 640..642
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 650..654
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 659..674
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 680..683
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 686..688
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 691..702
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 709..712
FT /evidence="ECO:0007829|PDB:2YOC"
FT TURN 720..722
FT /evidence="ECO:0007829|PDB:2YOC"
FT TURN 728..734
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 737..739
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 741..748
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 758..761
FT /evidence="ECO:0007829|PDB:2YOC"
FT TURN 765..771
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 781..795
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 799..801
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 803..805
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 811..813
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 814..816
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 817..819
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 822..824
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 827..829
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 830..832
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 833..835
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 840..842
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 845..852
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 859..874
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 876..883
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 886..888
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 898..900
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 902..905
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 918..920
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 924..927
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 928..930
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 931..938
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 946..964
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 967..969
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 974..980
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 981..985
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 994..1000
FT /evidence="ECO:0007829|PDB:2YOC"
FT TURN 1003..1005
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 1011..1021
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 1023..1025
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 1027..1029
FT /evidence="ECO:0007829|PDB:2YOC"
FT TURN 1031..1034
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 1041..1046
FT /evidence="ECO:0007829|PDB:2YOC"
FT HELIX 1047..1049
FT /evidence="ECO:0007829|PDB:2YOC"
FT TURN 1051..1054
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 1063..1065
FT /evidence="ECO:0007829|PDB:2YOC"
FT STRAND 1067..1076
FT /evidence="ECO:0007829|PDB:2YOC"
SQ SEQUENCE 1090 AA; 118098 MW; 240AE7DFB3FF1BD6 CRC64;
MLRYTRNALV LGSLVLLSGC DNGSSSSSSG NPDTPDNQDV VVRLPDVAVP GEAVTAVENQ
AVIHLVDIAG ITSSSAADYS SKNLYLWNNE TCDALSAPVA DWNDVSTTPS GSDKYGPYWV
IPLNKESGCI NVIVRDGTDK LIDSDLRVAF GDFTDRTVSV IAGNSAVYDS RADAFRAAFG
VALAEAHWVD KNTLLWPGGQ DKPIVRLYYS HSSKVAADGE GKFTDRYLKL TPTTVSQQVS
MRFPHLSSYA AFKLPDNANV DELLQGETVA IAAAEDGILI SATQVQTAGV LDDAYAEAAE
ALSYGAQLAD GGVTFRVWAP TAQQVDVVVY SADKKVIGSH PMTRDSASGA WSWQGGSDLK
GAFYRYAMTV YHPQSRKVEQ YEVTDPYAHS LSTNSEYSQV VDLNDSALKP DGWDNLTMPH
AQKTKADLAK MTIHESHIRD LSAWDQTVPA ELRGKYLALT AGDSNMVQHL KTLSASGVTH
VELLPVFDLA TVNEFSDKVA DIQQPFSRLC EVNSAVKSSE FAGYCDSGST VEEVLNQLKQ
SDSQDNPQVQ ALNTLVAQTD SYNWGYDPFH YTVPEGSYAT DPEGTTRIKE FRTMIQAIKQ
DLGMNVIMDV VYNHTNAAGP TDRTSVLDKI VPWYYQRLNE TTGSVESATC CSDSAPEHRM
FAKLIADSLA VWTTDYKIDG FRFDLMGYHP KAQILSAWER IKALNPDIYF FGEGWDSNQS
DRFEIASQIN LKGTGIGTFS DRLRDSVRGG GPFDSGDALR QNQGIGSGAG VLPNELASLS
DDQVRHLADL TRLGMAGNLA DFVMIDKDGA AKKGSEIDYN GAPGGYAADP TEVVNYVSKH
DNQTLWDMIS YKASQEADLA TRVRMQAVSL ATVMLGQGIA FDQQGSELLR SKSFTRDSYD
SGDWFNRVDY SLQDNNYNVG MPRISDDGSN YEVITRVKEM VATPGEAELK QMTAFYQELT
ELRKSSPLFT LGDGSAVMKR VDFRNTGSDQ QAGLLVMTVD DGMKAGASLD SRLDGLVVAI
NAAPESRTLN EFAGETLQLS AIQQTAGENS LANGVQIAAD GTVTLPAWSV AVLELPQGEA
QGAGLPVSSK
