ID   PULS_KLEPN              Reviewed;         125 AA.
AC   P20440;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Pullulanase secretion protein PulS;
DE   Flags: Precursor;
GN   Name=pulS;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2661532; DOI=10.1128/jb.171.7.3673-3679.1989;
RA   D'Enfert C., Pugsley A.P.;
RT   "Klebsiella pneumoniae pulS gene encodes an outer membrane lipoprotein
RT   required for pullulanase secretion.";
RL   J. Bacteriol. 171:3673-3679(1989).
CC   -!- FUNCTION: Involved in the secretion of pullulanase.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane; Lipid-anchor.
CC   -!- SIMILARITY: To E.chrysanthemi OutS. {ECO:0000305}.
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DR   EMBL; M29097; AAA61978.1; -; Genomic_DNA.
DR   PIR; C32880; C32880.
DR   PDB; 4A56; X-ray; 1.24 A; A=30-122.
DR   PDBsum; 4A56; -.
DR   AlphaFoldDB; P20440; -.
DR   SMR; P20440; -.
DR   TCDB; 8.A.2.1.1; the secretin auxiliary lipoprotein (sal) family.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR   Gene3D; 1.20.58.1630; -; 1.
DR   InterPro; IPR005699; Chap_lipoprot_PulS/OutS.
DR   InterPro; IPR019114; Chap_lipoprot_PulS/OutS-like.
DR   InterPro; IPR038432; PulS/OutS-like_sf.
DR   Pfam; PF09691; T2SS_PulS_OutS; 1.
DR   TIGRFAMs; TIGR01004; PulS_OutS; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell outer membrane; Lipoprotein; Membrane; Palmitate;
KW   Protein transport; Signal; Transport.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000305"
FT   CHAIN           18..125
FT                   /note="Pullulanase secretion protein PulS"
FT                   /id="PRO_0000018183"
FT   LIPID           18
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000305"
FT   LIPID           18
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   125 AA;  13808 MW;  450756F8FD98099D CRC64;
     MRNFILFPMM AVVLLSGCQQ NRPTTLSPAV SGQAQLEQLA SVAAGARYLK NKCNRSDLPA
     DEAINRAAIN VGKKRGWANI DANLLSQRSA QLYQQLQQDS TPEATKCSQF NRQLAPFIDS
     LRDNK