PUM10_ARATH
ID PUM10_ARATH Reviewed; 528 AA.
AC Q9LP21;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Putative pumilio homolog 10;
DE Short=APUM-10;
DE Short=AtPUM10;
GN Name=APUM10; OrderedLocusNames=At1g35750; ORFNames=F14D7.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA Francischini C.W., Quaggio R.B.;
RT "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT specificity and target candidates.";
RL FEBS J. 276:5456-5470(2009).
RN [4]
RP GENE FAMILY, AND SUBCELLULAR LOCATION.
RX PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT modeling, activity and subcellular localization.";
RL BMC Plant Biol. 10:44-44(2010).
CC -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:20214804}.
CC -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC together to form a right-handed superhelix that approximates a half
CC donut. The number as well as the specific sequence of the repeats
CC determine the specificity for target mRNAs (By similarity).
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79885.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC021198; AAF79885.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31827.1; -; Genomic_DNA.
DR PIR; F86479; F86479.
DR RefSeq; NP_174813.1; NM_103277.1.
DR AlphaFoldDB; Q9LP21; -.
DR SMR; Q9LP21; -.
DR STRING; 3702.AT1G35750.1; -.
DR iPTMnet; Q9LP21; -.
DR PaxDb; Q9LP21; -.
DR PRIDE; Q9LP21; -.
DR EnsemblPlants; AT1G35750.1; AT1G35750.1; AT1G35750.
DR GeneID; 840480; -.
DR Gramene; AT1G35750.1; AT1G35750.1; AT1G35750.
DR KEGG; ath:AT1G35750; -.
DR Araport; AT1G35750; -.
DR TAIR; locus:2011360; AT1G35750.
DR eggNOG; KOG2049; Eukaryota.
DR HOGENOM; CLU_004017_5_2_1; -.
DR InParanoid; Q9LP21; -.
DR OMA; NDAFHSS; -.
DR OrthoDB; 1364009at2759; -.
DR PhylomeDB; Q9LP21; -.
DR PRO; PR:Q9LP21; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LP21; baseline and differential.
DR Genevisible; Q9LP21; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR CDD; cd07920; Pumilio; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR033712; Pumilio_RNA-bd.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR Pfam; PF00806; PUF; 8.
DR SMART; SM00025; Pumilio; 8.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 7.
DR PROSITE; PS50303; PUM_HD; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Reference proteome; Repeat; RNA-binding; Translation regulation.
FT CHAIN 1..528
FT /note="Putative pumilio homolog 10"
FT /id="PRO_0000401392"
FT DOMAIN 188..528
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 213..248
FT /note="Pumilio 1"
FT REPEAT 249..284
FT /note="Pumilio 2"
FT REPEAT 285..323
FT /note="Pumilio 3"
FT REPEAT 325..360
FT /note="Pumilio 4"
FT REPEAT 361..396
FT /note="Pumilio 5"
FT REPEAT 397..433
FT /note="Pumilio 6"
FT REPEAT 434..465
FT /note="Pumilio 7"
FT REPEAT 466..503
FT /note="Pumilio 8"
SQ SEQUENCE 528 AA; 59381 MW; 7FBBC3A654FF7CC1 CRC64;
MEIFNFGQAS DHRRLPDFGS GGFLQSLDTN PFLKNQYYNN SVEALELCKK LNKMGISCDM
SIWTKPEEPF RVDPGDFGAK TLHESFGFDQ NLTGASQIHD GFRNFSSVRV QNNNFHGVSP
SPGLLGLQDS FNPNGFEEMM AFKDHKDFLL DHINEPIKRS PFLRGNDAFK GSLMFEGIRV
SQILAAMEGS GASYPDEPKI NGGLPLDLVS MVEIYGSVNL MARDQIGCRA LQKLVEEGTV
LDSKVIFLEI IDHVVELSMD PLGNYIVQKL LVVSDEEQRT MIVSVLTSKP RELIKICLNT
NGTRVIQKMI KTVKTKQQIA LVKSALEPGF LVLVNDSNGY HVLQSCLEFL VPNDNKFVVE
AATEYCAQLA THQYGCYVLQ CSLINTVGLQ HERLVAEISR DSLRLSQDPF GNYVVQCLID
QQVSSVNLLL PFRTHCIELA TQKFSSHVIE KCLRKYPESR AEIVRELLSY PNFEQLLQDP
YANYVIQTAL SVTKGAVRAR LVEKVKRFGK LQSNPYCKKI FSKTILKK
