PUM12_ARATH
ID PUM12_ARATH Reviewed; 596 AA.
AC Q9LVC3;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Pumilio homolog 12;
DE Short=APUM-12;
DE Short=AtPUM12;
GN Name=APUM12; OrderedLocusNames=At5g56510; ORFNames=MKN22.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA Francischini C.W., Quaggio R.B.;
RT "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT specificity and target candidates.";
RL FEBS J. 276:5456-5470(2009).
RN [4]
RP GENE FAMILY, AND SUBCELLULAR LOCATION.
RX PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT modeling, activity and subcellular localization.";
RL BMC Plant Biol. 10:44-44(2010).
CC -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:20214804}. Nucleus
CC {ECO:0000269|PubMed:20214804}.
CC -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC together to form a right-handed superhelix that approximates a half
CC donut. The number as well as the specific sequence of the repeats
CC determine the specificity for target mRNAs (By similarity).
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA97177.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB019234; BAA97177.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED96775.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM71040.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM71041.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM71042.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM71043.1; -; Genomic_DNA.
DR RefSeq; NP_001318812.1; NM_001345201.1.
DR RefSeq; NP_001332599.1; NM_001345204.1.
DR RefSeq; NP_001332600.1; NM_001345203.1.
DR RefSeq; NP_001332601.1; NM_001345202.1.
DR RefSeq; NP_200462.2; NM_125034.2.
DR AlphaFoldDB; Q9LVC3; -.
DR SMR; Q9LVC3; -.
DR BioGRID; 20996; 1.
DR STRING; 3702.AT5G56510.1; -.
DR PaxDb; Q9LVC3; -.
DR PRIDE; Q9LVC3; -.
DR ProteomicsDB; 224863; -.
DR EnsemblPlants; AT5G56510.1; AT5G56510.1; AT5G56510.
DR EnsemblPlants; AT5G56510.2; AT5G56510.2; AT5G56510.
DR EnsemblPlants; AT5G56510.3; AT5G56510.3; AT5G56510.
DR EnsemblPlants; AT5G56510.4; AT5G56510.4; AT5G56510.
DR EnsemblPlants; AT5G56510.6; AT5G56510.6; AT5G56510.
DR GeneID; 835752; -.
DR Gramene; AT5G56510.1; AT5G56510.1; AT5G56510.
DR Gramene; AT5G56510.2; AT5G56510.2; AT5G56510.
DR Gramene; AT5G56510.3; AT5G56510.3; AT5G56510.
DR Gramene; AT5G56510.4; AT5G56510.4; AT5G56510.
DR Gramene; AT5G56510.6; AT5G56510.6; AT5G56510.
DR KEGG; ath:AT5G56510; -.
DR Araport; AT5G56510; -.
DR TAIR; locus:2167111; AT5G56510.
DR eggNOG; KOG2049; Eukaryota.
DR HOGENOM; CLU_004017_3_0_1; -.
DR InParanoid; Q9LVC3; -.
DR OMA; FPICMSS; -.
DR OrthoDB; 1364009at2759; -.
DR PhylomeDB; Q9LVC3; -.
DR PRO; PR:Q9LVC3; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LVC3; baseline and differential.
DR Genevisible; Q9LVC3; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR CDD; cd07920; Pumilio; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR033712; Pumilio_RNA-bd.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR Pfam; PF00806; PUF; 8.
DR SMART; SM00025; Pumilio; 8.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 9.
DR PROSITE; PS50303; PUM_HD; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Nucleus; Reference proteome; Repeat; RNA-binding;
KW Translation regulation.
FT CHAIN 1..596
FT /note="Pumilio homolog 12"
FT /id="PRO_0000401394"
FT DOMAIN 254..596
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 277..312
FT /note="Pumilio 1"
FT REPEAT 313..348
FT /note="Pumilio 2"
FT REPEAT 349..388
FT /note="Pumilio 3"
FT REPEAT 389..424
FT /note="Pumilio 4"
FT REPEAT 425..460
FT /note="Pumilio 5"
FT REPEAT 461..496
FT /note="Pumilio 6"
FT REPEAT 497..532
FT /note="Pumilio 7"
FT REPEAT 533..570
FT /note="Pumilio 8"
SQ SEQUENCE 596 AA; 68671 MW; 3D8E134F7CF817ED CRC64;
MDQRRGNELD EFEKLLGEIP KVTSGNDYNH FPICLSSSRS QSIKKVDQYL PDDRAFTTSF
SEANLHFGIP NHTPESPHPL FINPSYHSPS NSPCVYDKFD SRKLDPVMFR KLQQVGYLPN
LSSGISPAQR QHYLPHSQPL SHYQSPMTWR DIEEENFQRL KLQEEQYLSI NPHFLHLQSM
DTVPRQDHFD YRRAEQSNRN LFWNGEDGNE SVRKMCYPEK ILMRSQMDLN TAKVIKYGAG
DESQNGRLWL QNQLNEDLTM SLNNLSLQPQ KYNSIAEARG KIYYLAKDQH GCRFLQRIFS
EKDGNDIEMI FNEIIDYISE LMMDPFGNYL VQKLLEVCNE DQRMQIVHSI TRKPGLLIKI
SCDMHGTRAV QKIVETAKRE EEISIIISAL KHGIVHLIKN VNGNHVVQRC LQYLLPYCGK
FLFEAAITHC VELATDRHGC CVLQKCLGYS EGEQKQHLVS EIASNALLLS QDPFGNYVLQ
YVFELQLQWA TFEILEQLEG NYTELSMQKC SSNVVEKCLK LADDKHRARI IRELINYGRL
DQVMLDPYGN YVIQAALKQS KGNVHALLVD AIKLNISSLR TNPYGKKVLS ALSSKK
