PUM13_ARATH
ID PUM13_ARATH Reviewed; 527 AA.
AC Q9FMH4;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Putative pumilio homolog 13;
DE Short=APUM-13;
DE Short=AtPUM13;
GN Name=APUM13; OrderedLocusNames=At5g43090; ORFNames=MMG4.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA Francischini C.W., Quaggio R.B.;
RT "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT specificity and target candidates.";
RL FEBS J. 276:5456-5470(2009).
RN [4]
RP GENE FAMILY.
RX PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT modeling, activity and subcellular localization.";
RL BMC Plant Biol. 10:44-44(2010).
CC -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC together to form a right-handed superhelix that approximates a half
CC donut. The number as well as the specific sequence of the repeats
CC determine the specificity for target mRNAs (By similarity).
CC {ECO:0000250}.
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DR EMBL; AB008267; BAB08273.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94909.1; -; Genomic_DNA.
DR RefSeq; NP_199123.1; NM_123675.2.
DR AlphaFoldDB; Q9FMH4; -.
DR SMR; Q9FMH4; -.
DR STRING; 3702.AT5G43090.1; -.
DR PaxDb; Q9FMH4; -.
DR PRIDE; Q9FMH4; -.
DR EnsemblPlants; AT5G43090.1; AT5G43090.1; AT5G43090.
DR GeneID; 834325; -.
DR Gramene; AT5G43090.1; AT5G43090.1; AT5G43090.
DR KEGG; ath:AT5G43090; -.
DR Araport; AT5G43090; -.
DR TAIR; locus:2167771; AT5G43090.
DR eggNOG; KOG2049; Eukaryota.
DR HOGENOM; CLU_533583_0_0_1; -.
DR InParanoid; Q9FMH4; -.
DR OMA; PFLENRW; -.
DR OrthoDB; 1364009at2759; -.
DR PhylomeDB; Q9FMH4; -.
DR PRO; PR:Q9FMH4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FMH4; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR CDD; cd07920; Pumilio; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR033712; Pumilio_RNA-bd.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR Pfam; PF00806; PUF; 5.
DR SMART; SM00025; Pumilio; 6.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 8.
DR PROSITE; PS50303; PUM_HD; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Reference proteome; Repeat; RNA-binding; Translation regulation.
FT CHAIN 1..527
FT /note="Putative pumilio homolog 13"
FT /id="PRO_0000401395"
FT DOMAIN 184..527
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 205..243
FT /note="Pumilio 1"
FT REPEAT 244..279
FT /note="Pumilio 2"
FT REPEAT 283..321
FT /note="Pumilio 3"
FT REPEAT 322..357
FT /note="Pumilio 4"
FT REPEAT 358..396
FT /note="Pumilio 5"
FT REPEAT 397..432
FT /note="Pumilio 6"
FT REPEAT 433..468
FT /note="Pumilio 7"
FT REPEAT 469..503
FT /note="Pumilio 8"
FT REGION 22..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..46
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 527 AA; 59937 MW; 1AC5F3D39C9B39A6 CRC64;
MDNKFYVNTN GERNIWSTAA EENMTTAASS SQSQPPQMQS SKFHQPENHI HINDGFSSGA
FDLQTLESSF RGLSFADSNV HQNGNPILPA HQYNQVFNGG GGSYGDGYLF PPSGSYHHYE
LKDLQRFNQN QQRLSYQNDY VNQSYRYDTI GGGNGMLNNS FLNGVPCASR NNVSDYYTNI
FGYGVNNSWR SNEGCTYNQD QAASSMENGR GSYFSIATDR VWSKELEKTI FVGTKETIDM
IFDGLIVGIC ELMVDPFGND VVKLLIGKCS SEQIILIVDV VTRHISKFVN ICFNPIGTLA
IQVLLTSIHE RANNQIPRIM DAISSVALQL TRNTNAKYVI LACFRMFTSS QCRRLLEVVS
QHCYQIAIDQ NGCCLLQQCF DKERVPNHEI RQRLISEVIE HALKLCLNCH GNYVVQYVVE
LDNQHETDLL VNKLLRNYAH LARNKYGSHV VQKLLKLRGI DSKLIVVDLL RGIDTLLLDP
FGNYVIQTAW FVSKEDVRQM LRYYIERNIR LMRCNKFGNK ILEKLNI
