PUM14_ARATH
ID PUM14_ARATH Reviewed; 518 AA.
AC Q4PSD1; Q9FMH2;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Pumilio homolog 14;
DE Short=APUM-14;
DE Short=AtPUM14;
GN Name=APUM14; OrderedLocusNames=At5g43110; ORFNames=MMG4.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP GENE FAMILY.
RX PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA Francischini C.W., Quaggio R.B.;
RT "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT specificity and target candidates.";
RL FEBS J. 276:5456-5470(2009).
RN [5]
RP GENE FAMILY.
RX PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT modeling, activity and subcellular localization.";
RL BMC Plant Biol. 10:44-44(2010).
CC -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
CC -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC together to form a right-handed superhelix that approximates a half
CC donut. The number as well as the specific sequence of the repeats
CC determine the specificity for target mRNAs (By similarity).
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08275.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB008267; BAB08275.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED94911.1; -; Genomic_DNA.
DR EMBL; DQ056705; AAY78851.1; -; mRNA.
DR RefSeq; NP_199125.2; NM_123677.2.
DR AlphaFoldDB; Q4PSD1; -.
DR SMR; Q4PSD1; -.
DR STRING; 3702.AT5G43110.1; -.
DR PaxDb; Q4PSD1; -.
DR PRIDE; Q4PSD1; -.
DR EnsemblPlants; AT5G43110.1; AT5G43110.1; AT5G43110.
DR GeneID; 834328; -.
DR Gramene; AT5G43110.1; AT5G43110.1; AT5G43110.
DR KEGG; ath:AT5G43110; -.
DR Araport; AT5G43110; -.
DR TAIR; locus:2167786; AT5G43110.
DR eggNOG; KOG2049; Eukaryota.
DR HOGENOM; CLU_533583_0_0_1; -.
DR InParanoid; Q4PSD1; -.
DR OMA; HECSSQQ; -.
DR OrthoDB; 1364009at2759; -.
DR PhylomeDB; Q4PSD1; -.
DR PRO; PR:Q4PSD1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q4PSD1; baseline and differential.
DR Genevisible; Q4PSD1; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR Pfam; PF00806; PUF; 6.
DR SMART; SM00025; Pumilio; 7.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 7.
DR PROSITE; PS50303; PUM_HD; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Nucleus; Reference proteome; Repeat; RNA-binding;
KW Translation regulation.
FT CHAIN 1..518
FT /note="Pumilio homolog 14"
FT /id="PRO_0000401396"
FT DOMAIN 178..518
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 206..235
FT /note="Pumilio 1; degenerate"
FT REPEAT 236..271
FT /note="Pumilio 2"
FT REPEAT 275..313
FT /note="Pumilio 3"
FT REPEAT 314..348
FT /note="Pumilio 4"
FT REPEAT 349..387
FT /note="Pumilio 5"
FT REPEAT 388..423
FT /note="Pumilio 6"
FT REPEAT 424..459
FT /note="Pumilio 7"
FT REPEAT 460..494
FT /note="Pumilio 8"
FT REGION 26..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 77..114
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 77..102
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 518 AA; 59449 MW; B230D565FE81C244 CRC64;
MDKNFRVNTN DERNIWLSTA VNENLTMASS SSQPQPISSP FHQPENQVNR VNPGSHYYDL
ETLESSFGGL SFNDSSVGQN GDSIHLPRRT NQVFTGSSSG GAGDDNGYLL PPMGSHHHRE
LEELQRHNYL NQLRMSYQND YAHQSYWYNT DGDGNGMLNN GFLNDVPSSS RDRVSDYYTN
RFGYEGYNYW RGNEGFDYNQ CQASFSAFAK DKEMSERLGM SIFQGTKETV DAIYNGLIGD
ICELMVDPYG SDVVQLLMRR CSSEQIVQLV DIVTQQMFQF VNICIDSLGT NAIQVLLTCI
NERAKDQIPR IVDVVRTVAL QLSKSNHAIF VILACFRLFP LHCRLLLELI VQNCHQIAID
QHGCCLLQLC FNKDRVPNLE IRQRLIMEAI ANALRLCLNC YGNYVVQYIV ELNNRYLIDA
LVRQLIGNYA HLARNKYGSH AVQKLLKLRW IDSRVIVIDL LREIDTLLLD PFGNYVIQTA
WFVSKDDVRR MLRYHIERNI PMMRCNKFGN KVLEKLNI
