PUM15_ARATH
ID PUM15_ARATH Reviewed; 444 AA.
AC P0DN95; O81465;
DT 10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2017, sequence version 1.
DT 03-AUG-2022, entry version 29.
DE RecName: Full=Pumilio homolog 15;
DE Short=APUM-15;
DE Short=AtPUM15;
GN Name=APUM15; OrderedLocusNames=At4g08560; ORFNames=T15F16.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA Francischini C.W., Quaggio R.B.;
RT "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT specificity and target candidates.";
RL FEBS J. 276:5456-5470(2009).
RN [4]
RP GENE FAMILY.
RX PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT modeling, activity and subcellular localization.";
RL BMC Plant Biol. 10:44-44(2010).
CC -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC together to form a right-handed superhelix that approximates a half
CC donut. The number as well as the specific sequence of the repeats
CC determine the specificity for target mRNAs (By similarity).
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC28191.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g08560 has been split into 2 genes: At4g08558 and At4g08560.; Evidence={ECO:0000305};
CC Sequence=AEE82658.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g08560 has been split into 2 genes: At4g08558 and At4g08560.; Evidence={ECO:0000305};
CC Sequence=CAB77981.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g08560 has been split into 2 genes: At4g08558 and At4g08560.; Evidence={ECO:0000305};
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DR EMBL; AF076275; AAC28191.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161512; CAB77981.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE82658.1; ALT_SEQ; Genomic_DNA.
DR PIR; T01828; T01828.
DR AlphaFoldDB; P0DN95; -.
DR SMR; P0DN95; -.
DR PRIDE; P0DN95; -.
DR Araport; AT4G08560; -.
DR eggNOG; KOG2049; Eukaryota.
DR PRO; PR:P0DN95; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; P0DN95; baseline.
DR Genevisible; O81465; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR CDD; cd07920; Pumilio; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR033712; Pumilio_RNA-bd.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR Pfam; PF00806; PUF; 5.
DR SMART; SM00025; Pumilio; 7.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 7.
DR PROSITE; PS50303; PUM_HD; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Reference proteome; Repeat; RNA-binding; Translation regulation.
FT CHAIN 1..444
FT /note="Pumilio homolog 15"
FT /id="PRO_0000401397"
FT DOMAIN 107..444
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 128..163
FT /note="Pumilio 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00317"
FT REPEAT 164..199
FT /note="Pumilio 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00317"
FT REPEAT 201..239
FT /note="Pumilio 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00317"
FT REPEAT 240..275
FT /note="Pumilio 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00317"
FT REPEAT 276..311
FT /note="Pumilio 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00317"
FT REPEAT 312..347
FT /note="Pumilio 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00317"
FT REPEAT 348..383
FT /note="Pumilio 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00317"
FT REPEAT 384..418
FT /note="Pumilio 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00317"
SQ SEQUENCE 444 AA; 51705 MW; 2A8136141FBC1DAF CRC64;
MSNQNERVAA APFQEAVESP AQRILWERLR TYDHSFPLSD YSPETLFSSD LQTLEHEFRR
FGVSELDRGD QDNYVNGYDA LRSFHERSRS ASVMGSPYGN LEDVPYLPSN PFFDQTPWSD
DAYGYMYGIR NTLLSRAKDG IESHMLQYVI AKGLKETIDK IFDNLISHVC ELMLDYYGHK
VFRKLMEKCT DEQITRVLDI VLEEPFEFVR LCVHTHGTHA IQGLMRSLCS EEQISRFMET
LCYVSLLLTK DVIAHRVILF CFNQFSPSHT RYLLEVIVQN CYQVAIDQNG CCMLKKLIRQ
SSRELRDPLI KEIISIAVRL CGNCYGNYVV QYLLRLKDYE VTSALSKHLD GNYVQLSYDK
YGSHVVQKCL ESREFSSRRI IAELLSDIDS LLVDPYGDYV IQTAWIVSED HVRHVLLFYI
NRNVPFMRCN VYGRKLLQKL NIWT
