ID   PUM16_ARATH             Reviewed;         332 AA.
AC   Q9FIE9;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 2.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Putative pumilio homolog 16;
DE            Short=APUM-16;
DE            Short=AtPUM16;
GN   Name=APUM16; OrderedLocusNames=At5g59280; ORFNames=MNC17.19;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA   Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT   features of the regions of 1,081,958 bp covered by seventeen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:379-391(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY.
RX   PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA   Francischini C.W., Quaggio R.B.;
RT   "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT   specificity and target candidates.";
RL   FEBS J. 276:5456-5470(2009).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA   Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT   "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT   modeling, activity and subcellular localization.";
RL   BMC Plant Biol. 10:44-44(2010).
CC   -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC       translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC       together to form a right-handed superhelix that approximates a half
CC       donut. The number as well as the specific sequence of the repeats
CC       determine the specificity for target mRNAs (By similarity).
CC       {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB09773.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB016890; BAB09773.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED97165.1; -; Genomic_DNA.
DR   RefSeq; NP_200736.1; NM_125318.2.
DR   AlphaFoldDB; Q9FIE9; -.
DR   SMR; Q9FIE9; -.
DR   STRING; 3702.AT5G59280.1; -.
DR   PaxDb; Q9FIE9; -.
DR   PRIDE; Q9FIE9; -.
DR   EnsemblPlants; AT5G59280.1; AT5G59280.1; AT5G59280.
DR   GeneID; 836046; -.
DR   Gramene; AT5G59280.1; AT5G59280.1; AT5G59280.
DR   KEGG; ath:AT5G59280; -.
DR   Araport; AT5G59280; -.
DR   TAIR; locus:2168529; AT5G59280.
DR   eggNOG; KOG2049; Eukaryota.
DR   HOGENOM; CLU_048501_0_0_1; -.
DR   InParanoid; Q9FIE9; -.
DR   OMA; RCTRNIA; -.
DR   OrthoDB; 1191728at2759; -.
DR   PRO; PR:Q9FIE9; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FIE9; baseline and differential.
DR   Genevisible; Q9FIE9; AT.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR   GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR033133; PUM-HD.
DR   InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR   Pfam; PF00806; PUF; 3.
DR   SMART; SM00025; Pumilio; 5.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS50302; PUM; 5.
DR   PROSITE; PS50303; PUM_HD; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; Repeat; RNA-binding; Translation regulation.
FT   CHAIN           1..332
FT                   /note="Putative pumilio homolog 16"
FT                   /id="PRO_0000401398"
FT   DOMAIN          1..329
FT                   /note="PUM-HD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT   REPEAT          82..117
FT                   /note="Pumilio 1"
FT   REPEAT          118..152
FT                   /note="Pumilio 2; degenerate"
FT   REPEAT          153..188
FT                   /note="Pumilio 3"
FT   REPEAT          189..225
FT                   /note="Pumilio 4"
FT   REPEAT          226..264
FT                   /note="Pumilio 5"
FT   REPEAT          265..300
FT                   /note="Pumilio 6"
SQ   SEQUENCE   332 AA;  37977 MW;  97F510BCCFAC1285 CRC64;
     MANNNSFSMS TMFNTIQEIL NEEPLTVPSP PSRGGYSLFH IRKAEMDIQR IFNFMTGSED
     LKDDISVLDT GTLMWMASLM TSDCDYFMVI TTNKNGSKTL QKLMGMSDDM DVFFFEAIMR
     LFIHVMTDKY ASYVTIQGMR VFQQDKRELM YDQILRYACF LAGDQYGCIA LNEIITDLDD
     PYYRDQLLDI VANNALLLSN DAYGNFVVQH VLKLRDSRCT RNIADKLCGY CVELSFKKYG
     SYIVERLLEA GDVPMATVVL DLLACKTEML IRLARSEYGN FVVCKALELT NEILTADLFY
     GLVNRLMPYR HLLHRSPRSK IATILDSMSV PN