ID   PUM18_ARATH             Reviewed;         327 AA.
AC   Q9LVG3;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Pumilio homolog 18;
DE            Short=APUM-18;
DE            Short=AtPUM18;
GN   Name=APUM18; OrderedLocusNames=At5g60110; ORFNames=MGO3.9;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT   features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:31-63(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA   Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL   Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY.
RX   PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA   Francischini C.W., Quaggio R.B.;
RT   "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT   specificity and target candidates.";
RL   FEBS J. 276:5456-5470(2009).
RN   [5]
RP   GENE FAMILY, AND SUBCELLULAR LOCATION.
RX   PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA   Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT   "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT   modeling, activity and subcellular localization.";
RL   BMC Plant Biol. 10:44-44(2010).
CC   -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC       translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:20214804}.
CC   -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC       together to form a right-handed superhelix that approximates a half
CC       donut. The number as well as the specific sequence of the repeats
CC       determine the specificity for target mRNAs (By similarity).
CC       {ECO:0000250}.
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DR   EMBL; AB019231; BAA96940.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED97279.1; -; Genomic_DNA.
DR   EMBL; DQ056729; AAY78873.1; -; mRNA.
DR   RefSeq; NP_200819.1; NM_125404.1.
DR   AlphaFoldDB; Q9LVG3; -.
DR   SMR; Q9LVG3; -.
DR   PaxDb; Q9LVG3; -.
DR   PRIDE; Q9LVG3; -.
DR   EnsemblPlants; AT5G60110.1; AT5G60110.1; AT5G60110.
DR   GeneID; 836133; -.
DR   Gramene; AT5G60110.1; AT5G60110.1; AT5G60110.
DR   KEGG; ath:AT5G60110; -.
DR   Araport; AT5G60110; -.
DR   TAIR; locus:2164165; AT5G60110.
DR   eggNOG; KOG2049; Eukaryota.
DR   HOGENOM; CLU_048501_0_0_1; -.
DR   InParanoid; Q9LVG3; -.
DR   OMA; SNMIFSW; -.
DR   OrthoDB; 622215at2759; -.
DR   PhylomeDB; Q9LVG3; -.
DR   PRO; PR:Q9LVG3; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9LVG3; baseline and differential.
DR   Genevisible; Q9LVG3; AT.
DR   GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR   GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR   GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR033133; PUM-HD.
DR   InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR   Pfam; PF00806; PUF; 3.
DR   SMART; SM00025; Pumilio; 6.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS50302; PUM; 5.
DR   PROSITE; PS50303; PUM_HD; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Reference proteome; Repeat; RNA-binding; Translation regulation.
FT   CHAIN           1..327
FT                   /note="Pumilio homolog 18"
FT                   /id="PRO_0000401400"
FT   DOMAIN          1..324
FT                   /note="PUM-HD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT   REPEAT          79..114
FT                   /note="Pumilio 1"
FT   REPEAT          115..149
FT                   /note="Pumilio 2"
FT   REPEAT          150..185
FT                   /note="Pumilio 3"
FT   REPEAT          186..222
FT                   /note="Pumilio 4"
FT   REPEAT          223..260
FT                   /note="Pumilio 5"
FT   REPEAT          261..295
FT                   /note="Pumilio 6"
SQ   SEQUENCE   327 AA;  36838 MW;  0133C5B5C96E05DA CRC64;
     MAVADNPFSM STMFNALPNP KGISGSIPPP GFAPRASATP LHAALFNLMT DGDGVSYFKE
     MISNSDKTEL QRMASLLTSD SDYFMSIVTT KFGSRRVQKL LGKSDDVDAF FCAAILRRFL
     HITTDKYASY VTIRAMVVFD KVMKKALYER ILYHALDLAC DQHGCIALND IITDADDPYY
     RDQLLELVAS NALRLSNDAS GNFVVQHVLT LYDSRCIHNI AVNLYGQCIE LSFKKYGSYI
     VEKLLEVEES MVVVVVELLG CDGDRLMRLA RNEFGNFVVV KALRFTKMSR MDLFWGLVQK
     LMPFIRLLRR SHGSNIANIL DSFRLRC