ID   PUM19_ARATH             Reviewed;         327 AA.
AC   Q9LSS8;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 2.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Putative pumilio homolog 19;
DE            Short=APUM-19;
DE            Short=AtPUM19;
GN   Name=APUM19; OrderedLocusNames=At5g60180; ORFNames=F15L12.7;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RA   Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL   Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY.
RX   PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA   Francischini C.W., Quaggio R.B.;
RT   "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT   specificity and target candidates.";
RL   FEBS J. 276:5456-5470(2009).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA   Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT   "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT   modeling, activity and subcellular localization.";
RL   BMC Plant Biol. 10:44-44(2010).
CC   -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC       translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC       together to form a right-handed superhelix that approximates a half
CC       donut. The number as well as the specific sequence of the repeats
CC       determine the specificity for target mRNAs (By similarity).
CC       {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA97499.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AB026632; BAA97499.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP002688; AED97290.1; -; Genomic_DNA.
DR   RefSeq; NP_200826.1; NM_125411.2.
DR   AlphaFoldDB; Q9LSS8; -.
DR   SMR; Q9LSS8; -.
DR   PaxDb; Q9LSS8; -.
DR   PRIDE; Q9LSS8; -.
DR   EnsemblPlants; AT5G60180.1; AT5G60180.1; AT5G60180.
DR   GeneID; 836140; -.
DR   Gramene; AT5G60180.1; AT5G60180.1; AT5G60180.
DR   KEGG; ath:AT5G60180; -.
DR   Araport; AT5G60180; -.
DR   TAIR; locus:2144010; AT5G60180.
DR   eggNOG; KOG2049; Eukaryota.
DR   HOGENOM; CLU_048501_0_0_1; -.
DR   InParanoid; Q9LSS8; -.
DR   OMA; KLMPVRH; -.
DR   OrthoDB; 945054at2759; -.
DR   PhylomeDB; Q9LSS8; -.
DR   PRO; PR:Q9LSS8; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9LSS8; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR   GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR033133; PUM-HD.
DR   InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR   Pfam; PF00806; PUF; 3.
DR   SMART; SM00025; Pumilio; 6.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS50302; PUM; 5.
DR   PROSITE; PS50303; PUM_HD; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; Repeat; RNA-binding; Translation regulation.
FT   CHAIN           1..327
FT                   /note="Putative pumilio homolog 19"
FT                   /id="PRO_0000401401"
FT   DOMAIN          1..324
FT                   /note="PUM-HD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT   REPEAT          79..114
FT                   /note="Pumilio 1"
FT   REPEAT          115..149
FT                   /note="Pumilio 2"
FT   REPEAT          150..185
FT                   /note="Pumilio 3"
FT   REPEAT          186..222
FT                   /note="Pumilio 4"
FT   REPEAT          223..260
FT                   /note="Pumilio 5"
FT   REPEAT          261..295
FT                   /note="Pumilio 6"
SQ   SEQUENCE   327 AA;  37063 MW;  AF82EE9AECB0F299 CRC64;
     MAVSDNTFSM STMFNALPNP TETSGYIPPS GFAPRASATP LHAALFNLMT DGDGVSYFKE
     MISKSDKTEL QRMASLLTSD SDYFMSIVTT KFGSRRVQKL LGKSDDVDAF FCAAILRRFL
     HITTDKYASY VTIRAMVVFD KVMKKALYER ILYHALDLAC DQHGCIALND IITDADDPYY
     RDQLLELVVS NALRLSNDAS GNFVVQHVLT LYDSRCIHNI AVNLYGQCIE LSFKKYGSYI
     VEKLLEVEES MVVVVVELLG CDGDRLMRLA RNEFGNFVVV KALRFTKEMR MDLFWGLVQK
     LMPFIHLLRR SHGNNIANIL DTFRLRC