PUM1_ARATH
ID PUM1_ARATH Reviewed; 968 AA.
AC Q9ZW07;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Pumilio homolog 1;
DE Short=APUM-1;
DE Short=AtPUM1;
GN Name=APUM1; OrderedLocusNames=At2g29200; ORFNames=F16P2.42;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, FUNCTION, AND RNA-BINDING.
RX PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA Francischini C.W., Quaggio R.B.;
RT "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT specificity and target candidates.";
RL FEBS J. 276:5456-5470(2009).
RN [4]
RP GENE FAMILY.
RX PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT modeling, activity and subcellular localization.";
RL BMC Plant Biol. 10:44-44(2010).
CC -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC Binds the APUM-binding elements (APBEs) in the 3'-UTR mRNA sequence of
CC CLV1, PNH, WUS and FAS2. {ECO:0000269|PubMed:19682068}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC together to form a right-handed superhelix that approximates a half
CC donut. The number as well as the specific sequence of the repeats
CC determine the specificity for target mRNAs (By similarity).
CC {ECO:0000250}.
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DR EMBL; AC004561; AAC95215.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08222.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62387.1; -; Genomic_DNA.
DR PIR; F84693; F84693.
DR RefSeq; NP_001324547.1; NM_001336203.1.
DR RefSeq; NP_180483.1; NM_128476.3.
DR AlphaFoldDB; Q9ZW07; -.
DR SMR; Q9ZW07; -.
DR STRING; 3702.AT2G29200.1; -.
DR iPTMnet; Q9ZW07; -.
DR PaxDb; Q9ZW07; -.
DR PRIDE; Q9ZW07; -.
DR ProteomicsDB; 226458; -.
DR EnsemblPlants; AT2G29200.1; AT2G29200.1; AT2G29200.
DR EnsemblPlants; AT2G29200.2; AT2G29200.2; AT2G29200.
DR GeneID; 817469; -.
DR Gramene; AT2G29200.1; AT2G29200.1; AT2G29200.
DR Gramene; AT2G29200.2; AT2G29200.2; AT2G29200.
DR KEGG; ath:AT2G29200; -.
DR Araport; AT2G29200; -.
DR TAIR; locus:2043182; AT2G29200.
DR eggNOG; KOG1488; Eukaryota.
DR HOGENOM; CLU_004017_1_1_1; -.
DR InParanoid; Q9ZW07; -.
DR OMA; QHINGAN; -.
DR OrthoDB; 206600at2759; -.
DR PhylomeDB; Q9ZW07; -.
DR PRO; PR:Q9ZW07; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZW07; baseline and differential.
DR Genevisible; Q9ZW07; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IDA:UniProtKB.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR CDD; cd07920; Pumilio; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR012940; NABP.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR033712; Pumilio_RNA-bd.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR Pfam; PF07990; NABP; 2.
DR Pfam; PF00806; PUF; 8.
DR SMART; SM00025; Pumilio; 8.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 9.
DR PROSITE; PS50303; PUM_HD; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome; Repeat; RNA-binding;
KW Translation regulation.
FT CHAIN 1..968
FT /note="Pumilio homolog 1"
FT /id="PRO_0000401383"
FT DOMAIN 610..950
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 630..665
FT /note="Pumilio 1"
FT REPEAT 666..701
FT /note="Pumilio 2"
FT REPEAT 702..737
FT /note="Pumilio 3"
FT REPEAT 738..773
FT /note="Pumilio 4"
FT REPEAT 774..810
FT /note="Pumilio 5"
FT REPEAT 811..846
FT /note="Pumilio 6"
FT REPEAT 847..882
FT /note="Pumilio 7"
FT REPEAT 883..924
FT /note="Pumilio 8"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 138..171
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 204..240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 260..303
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 360..382
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..25
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 213..240
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 278..303
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 363..380
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 194
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9SS47"
FT MOD_RES 261
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9ZW02"
SQ SEQUENCE 968 AA; 106563 MW; 799D230642083A6E CRC64;
MIPELGRRPM HRGNEDSSFG DDYEKEIGVL LGEQQRRQVE ADELERELNL FRSGSAPPTV
DGSVSAAGGL FSGGGGAPFL EFGGVNKGNG FGGDDEEFRK DPAYLSYYYA NMKLNPRLPP
PLMSREDLRV AQRLKGSNNV LGGVGDRRKV NDNRSLFSMP PGFEGEKTGA SASEWDANGL
IGLPGLGLGG KQKSFADIFQ ADMGHGHPVA QQPSRPASRN TFDENVDSNN NLSPSASQGI
GAPSPYSYAA VLGSSLSRNG TPDPQAIARV PSPCLTPIGS GRMSSNDKRN TSNQSPFNGV
TSGLNESSDL VNALSGLNLS CSVGLDDRSQ AEQDVEKVRN YMFGLQGGHN EVNQHEFPNK
SDQAHKATGS LRNSQLRGPH GSAYNGGVGL ANPYQQLDSP NYCLNNYALN PAVASMMANQ
LGNNNFAPMY DNVSALGFSG MDSRHHGRGF VSSGQNLSES RNLGRFSNRM MGGGAGLQSH
MVDPMYNQYA DSLDLLNDPS MDRNFMGGSS YMDMLELQRA YLGAQKSQYG VPYKSGSPNS
HSYYGSPTFG SNMSYPGSPL AHHGMPNSLM SPYSPMRRDE VNMRFPSATR NYSGGLMGSW
HMDASFDEGF GSSMLEEFKS NKTRGFELSE IAGHVVEFSS DQYGSRFIQQ KLETATTDEK
NMVYEEIMPQ ALVLMTDVFG NYVIQKFFEH GLPPQRRELA EKLFDHVLPL SLQMYGCRVI
QKAIEVVDLD QKIKMVKELD GHVMRCVRDQ NGNHVVQKCI ECVPEENIEF IISTFFGHVV
TLSTHPYGCR VIQRVLEHCH DPDTQSKVME EILSTVSMLA QDQYGNYVVQ HVLEHGKPDE
RTVIIKELAG KIVQMSQQKF ASNVVEKCLT FGGPEERELL VNEMLGTTDE NEPLQAMMKD
QFANYVVQKV LETCDDQQRE LILTRIKVHL TALKKYTYGK HVVARIEKLV AAGERRMALQ
SLTQPQMA
