PUM20_ARATH
ID PUM20_ARATH Reviewed; 332 AA.
AC Q9XI17; F4HY31;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Putative pumilio homolog 20;
DE Short=APUM-20;
DE Short=AtPUM20;
GN Name=APUM20; OrderedLocusNames=At1g21620; ORFNames=F24J8.22, F8K7.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA Francischini C.W., Quaggio R.B.;
RT "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT specificity and target candidates.";
RL FEBS J. 276:5456-5470(2009).
RN [4]
RP GENE FAMILY.
RX PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT modeling, activity and subcellular localization.";
RL BMC Plant Biol. 10:44-44(2010).
CC -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC together to form a right-handed superhelix that approximates a half
CC donut. The number as well as the specific sequence of the repeats
CC determine the specificity for target mRNAs (By similarity).
CC {ECO:0000250}.
CC -!- CAUTION: Could be the product of a pseudogene. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD41414.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAD41414.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AC015447; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AEE30127.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007727; AAD41414.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC015447; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE30127.1; ALT_SEQ; Genomic_DNA.
DR PIR; B86349; B86349.
DR RefSeq; NP_173581.1; NM_102011.1.
DR AlphaFoldDB; Q9XI17; -.
DR SMR; Q9XI17; -.
DR PRIDE; Q9XI17; -.
DR GeneID; 838764; -.
DR KEGG; ath:AT1G21620; -.
DR Araport; AT1G21620; -.
DR eggNOG; KOG2049; Eukaryota.
DR HOGENOM; CLU_048501_0_0_1; -.
DR InParanoid; Q9XI17; -.
DR OrthoDB; 1705222at2759; -.
DR PhylomeDB; Q9XI17; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9XI17; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR Pfam; PF00806; PUF; 3.
DR SMART; SM00025; Pumilio; 4.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 5.
DR PROSITE; PS50303; PUM_HD; 1.
PE 5: Uncertain;
KW Cytoplasm; Reference proteome; Repeat; RNA-binding; Translation regulation.
FT CHAIN 1..332
FT /note="Putative pumilio homolog 20"
FT /id="PRO_0000401402"
FT DOMAIN 1..332
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 89..124
FT /note="Pumilio 1"
FT REPEAT 125..159
FT /note="Pumilio 2"
FT REPEAT 160..191
FT /note="Pumilio 3; degenerate"
FT REPEAT 192..228
FT /note="Pumilio 4"
FT REPEAT 229..266
FT /note="Pumilio 5"
FT REPEAT 267..303
FT /note="Pumilio 6"
SQ SEQUENCE 332 AA; 38112 MW; AAD8AAD2ABE8469D CRC64;
MAHQLRFAAA REPISEEYPA VALPHPRCIS VAFPPPRFTP SPSPLTNYSF LAALTPQELE
MRLQIAMDVQ MISKLDQRKL QTMASLLTSD PDYFLMIARN MNGSKRIQKL LGKTDDVDAL
FAAAILRRFL HIITDKYASY VVRRGMTVFD KKKKKAMYEH ILHYASHIAR DKHGNLALND
IITDAYRNKL FDVIAHKALV LSNDAYGNFV IQRVLKLNDL RSKNNIVVSL RGHFVDLSFQ
KYGSYVVDVL LETKESMVVV VEELMECEGD MLMRLARNEY GNFLVCKALR VTQKEMVRTD
LFWGLVHKLK PFHNLLRWSR GKNIASILNS IR
