PUM21_ARATH
ID PUM21_ARATH Reviewed; 517 AA.
AC Q9LXC5;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Putative pumilio homolog 21;
DE Short=APUM-21;
DE Short=AtPUM21;
GN Name=APUM21; OrderedLocusNames=At5g09610; ORFNames=F17I14_200, MTH16.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY.
RX PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA Francischini C.W., Quaggio R.B.;
RT "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT specificity and target candidates.";
RL FEBS J. 276:5456-5470(2009).
RN [5]
RP GENE FAMILY.
RX PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT modeling, activity and subcellular localization.";
RL BMC Plant Biol. 10:44-44(2010).
CC -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC together to form a right-handed superhelix that approximates a half
CC donut. The number as well as the specific sequence of the repeats
CC determine the specificity for target mRNAs (By similarity).
CC {ECO:0000250}.
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DR EMBL; AB020752; BAB09516.1; -; Genomic_DNA.
DR EMBL; AL353994; CAB89369.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91417.1; -; Genomic_DNA.
DR PIR; T49937; T49937.
DR RefSeq; NP_196523.1; NM_120998.2.
DR AlphaFoldDB; Q9LXC5; -.
DR SMR; Q9LXC5; -.
DR PaxDb; Q9LXC5; -.
DR PRIDE; Q9LXC5; -.
DR ProteomicsDB; 226113; -.
DR EnsemblPlants; AT5G09610.1; AT5G09610.1; AT5G09610.
DR GeneID; 830820; -.
DR Gramene; AT5G09610.1; AT5G09610.1; AT5G09610.
DR KEGG; ath:AT5G09610; -.
DR Araport; AT5G09610; -.
DR TAIR; locus:2144711; AT5G09610.
DR eggNOG; KOG2049; Eukaryota.
DR HOGENOM; CLU_040232_0_0_1; -.
DR InParanoid; Q9LXC5; -.
DR OMA; LYANYNH; -.
DR OrthoDB; 1036421at2759; -.
DR PhylomeDB; Q9LXC5; -.
DR PRO; PR:Q9LXC5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LXC5; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR Pfam; PF00806; PUF; 4.
DR SMART; SM00025; Pumilio; 4.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 4.
DR PROSITE; PS50303; PUM_HD; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Reference proteome; Repeat; RNA-binding; Translation regulation.
FT CHAIN 1..517
FT /note="Putative pumilio homolog 21"
FT /id="PRO_0000401403"
FT DOMAIN 148..502
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 258..293
FT /note="Pumilio 1; degenerate"
FT REPEAT 294..328
FT /note="Pumilio 2"
FT REPEAT 329..363
FT /note="Pumilio 3; degenerate"
FT REPEAT 364..400
FT /note="Pumilio 4"
FT REPEAT 401..437
FT /note="Pumilio 5"
FT REPEAT 438..473
FT /note="Pumilio 6"
FT REGION 33..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 69..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 107..130
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 175..201
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 33..56
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 107..129
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 175..189
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 517 AA; 58776 MW; 4846D1CBF6A84C07 CRC64;
MADDNGKNPM PNDRGYDTLL TAFNNLKLYA NYNHQEPGES GNTNINSNNN HPNPPLLTAF
KSLKLYANKN NQEPGESGNT TINRNKNHPN PPLVTAFNNL ELYANYNHQE PGESGTTNIN
NNNPNPPLLT AFDNLRLYAN YNHQEPGESS NNNYPNLNVY NVGHISAASF NAPPFTPSSL
TQPDDSSSRY SGKPFPPPPL SFVSPGVDKD RNWLSSLLDM MTCAQRFTEF QKYLQDLDTY
PTAERESHLF KIGSALTTTK RIFLHLATNQ YGSQALRILF RRSPSLDHLL FCAVDTNFFL
LMSDKYGRGL IIPAIRAVDK TKKESLYKLT YEYTLHLARL ETGCLALNNV LQEIRGIYRD
LIFECVANNA DWLSFDPYGT HVVQNILILQ NPVATTAIAE RLRGSFFRLA MERQGSYVVE
KCLKSDFARD QVLEEFRGNA KEWVRMTTDK FGNFVVQSAL RVMKEKEMRP LLREFVEKLR
PHFGKMEIGR GRNTLRVIQE EIVGWINQLP DKSGYVN
