PUM22_ARATH
ID PUM22_ARATH Reviewed; 361 AA.
AC Q9LNI2;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Putative pumilio homolog 22;
DE Short=APUM-22;
DE Short=AtPUM22;
GN Name=APUM22; OrderedLocusNames=At1g01410; ORFNames=F6F3.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA Francischini C.W., Quaggio R.B.;
RT "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT specificity and target candidates.";
RL FEBS J. 276:5456-5470(2009).
RN [4]
RP GENE FAMILY.
RX PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT modeling, activity and subcellular localization.";
RL BMC Plant Biol. 10:44-44(2010).
CC -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC together to form a right-handed superhelix that approximates a half
CC donut. The number as well as the specific sequence of the repeats
CC determine the specificity for target mRNAs (By similarity).
CC {ECO:0000250}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC023628; AAF97334.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27283.1; -; Genomic_DNA.
DR PIR; F86144; F86144.
DR RefSeq; NP_171648.1; NM_100023.1.
DR AlphaFoldDB; Q9LNI2; -.
DR SMR; Q9LNI2; -.
DR STRING; 3702.AT1G01410.1; -.
DR PaxDb; Q9LNI2; -.
DR PRIDE; Q9LNI2; -.
DR EnsemblPlants; AT1G01410.1; AT1G01410.1; AT1G01410.
DR GeneID; 837547; -.
DR Gramene; AT1G01410.1; AT1G01410.1; AT1G01410.
DR KEGG; ath:AT1G01410; -.
DR Araport; AT1G01410; -.
DR TAIR; locus:2035312; AT1G01410.
DR eggNOG; KOG2049; Eukaryota.
DR HOGENOM; CLU_048501_2_0_1; -.
DR InParanoid; Q9LNI2; -.
DR OMA; HHKKLFC; -.
DR OrthoDB; 945054at2759; -.
DR PhylomeDB; Q9LNI2; -.
DR PRO; PR:Q9LNI2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LNI2; differential.
DR Genevisible; Q9LNI2; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR SMART; SM00025; Pumilio; 3.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 5.
DR PROSITE; PS50303; PUM_HD; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Reference proteome; Repeat; RNA-binding; Translation regulation.
FT CHAIN 1..361
FT /note="Putative pumilio homolog 22"
FT /id="PRO_0000401404"
FT DOMAIN 5..348
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 27..63
FT /note="Pumilio 1"
FT REPEAT 64..103
FT /note="Pumilio 2"
FT REPEAT 104..131
FT /note="Pumilio 3; degenerate"
FT REPEAT 132..167
FT /note="Pumilio 4"
FT REPEAT 168..205
FT /note="Pumilio 5; degenerate"
FT REPEAT 206..243
FT /note="Pumilio 6; degenerate"
FT REPEAT 244..284
FT /note="Pumilio 7"
FT REPEAT 285..319
FT /note="Pumilio 8"
SQ SEQUENCE 361 AA; 41255 MW; 7D85508941A3ADDC CRC64;
MTHERGYDLA SQVLNICNVH HKKLFCHITY RHLLVLSSDD NGCVILKKVI TIADDFLKDE
FLDLIAQHAH SLSMHDLGIS LIQHVLELDF TKKTTQDDKR LHELMAEFDE VLSTSVTADV
DKLHKLASKL MLDSDLFFEF VITRRGSLMI QIILGKSEEV DQVILAGVKQ RFIDVTTNFY
GYRIMIQTIK VFKKRGDLKV YDQILRLIGV HALYLTKDPD MGNKTFQHAI NLHHQDCTTF
IACGLQSHYI ELSFLKHGSK IVEMLIDDRI SMVPLVLLMM EIVKCDEDTL VRLATDEYGN
NILKKFLALA KEHKEDFFGD LVDKLNPLLD SLRGTLGENI VAIIDSETEM VKDRIVSQGN
N
