PUM24_ARATH
ID PUM24_ARATH Reviewed; 641 AA.
AC Q9LRZ3;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 124.
DE RecName: Full=Pumilio homolog 24;
DE Short=APUM-24;
DE Short=AtPUM24;
GN Name=APUM24; OrderedLocusNames=At3g16810; ORFNames=K20I9_3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=17272265; DOI=10.1074/mcp.m600408-mcp200;
RA Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.;
RT "Multidimensional protein identification technology (MudPIT) analysis of
RT ubiquitinated proteins in plants.";
RL Mol. Cell. Proteomics 6:601-610(2007).
RN [5]
RP GENE FAMILY.
RX PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA Francischini C.W., Quaggio R.B.;
RT "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT specificity and target candidates.";
RL FEBS J. 276:5456-5470(2009).
RN [6]
RP GENE FAMILY, AND SUBCELLULAR LOCATION.
RX PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT modeling, activity and subcellular localization.";
RL BMC Plant Biol. 10:44-44(2010).
CC -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:20214804}.
CC -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC together to form a right-handed superhelix that approximates a half
CC donut. The number as well as the specific sequence of the repeats
CC determine the specificity for target mRNAs (By similarity).
CC {ECO:0000250}.
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DR EMBL; AB028608; BAA95774.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75869.1; -; Genomic_DNA.
DR EMBL; AY045638; AAK73996.1; -; mRNA.
DR RefSeq; NP_566559.1; NM_112555.2.
DR AlphaFoldDB; Q9LRZ3; -.
DR SMR; Q9LRZ3; -.
DR IntAct; Q9LRZ3; 1.
DR STRING; 3702.AT3G16810.1; -.
DR iPTMnet; Q9LRZ3; -.
DR PaxDb; Q9LRZ3; -.
DR PRIDE; Q9LRZ3; -.
DR ProteomicsDB; 226115; -.
DR EnsemblPlants; AT3G16810.1; AT3G16810.1; AT3G16810.
DR GeneID; 820934; -.
DR Gramene; AT3G16810.1; AT3G16810.1; AT3G16810.
DR KEGG; ath:AT3G16810; -.
DR Araport; AT3G16810; -.
DR TAIR; locus:2086760; AT3G16810.
DR eggNOG; KOG2050; Eukaryota.
DR HOGENOM; CLU_016751_0_0_1; -.
DR InParanoid; Q9LRZ3; -.
DR OMA; CNTYAVH; -.
DR OrthoDB; 845051at2759; -.
DR PhylomeDB; Q9LRZ3; -.
DR PRO; PR:Q9LRZ3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LRZ3; baseline and differential.
DR Genevisible; Q9LRZ3; AT.
DR GO; GO:0005730; C:nucleolus; IDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IDA:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0006417; P:regulation of translation; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR012959; CPL_dom.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR040059; PUM3.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR PANTHER; PTHR13389; PTHR13389; 1.
DR Pfam; PF08144; CPL; 1.
DR Pfam; PF00806; PUF; 3.
DR SMART; SM00025; Pumilio; 5.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 6.
DR PROSITE; PS50303; PUM_HD; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Repeat; RNA-binding; Translation regulation.
FT CHAIN 1..641
FT /note="Pumilio homolog 24"
FT /id="PRO_0000401406"
FT DOMAIN 9..404
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 118..153
FT /note="Pumilio 1"
FT REPEAT 154..189
FT /note="Pumilio 2"
FT REPEAT 190..225
FT /note="Pumilio 3"
FT REPEAT 303..340
FT /note="Pumilio 4"
FT REPEAT 341..378
FT /note="Pumilio 5"
FT REGION 1..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 427..468
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..36
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..82
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 452..468
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 641 AA; 71604 MW; 990C6E9EDE22A9DB CRC64;
MSSKGLKPQK STKRKDTDSS AKFDSLKSKK PKLVSGEQQQ HVKPKFGKPK SAGDKEQSTN
LSKKERRVQA KELTEARKKK RKPHYNLEQE LVSLWEKMRR RNIGKEDRSK LISEAIRKMK
GKVPEIAVSH VSSRVLQTCV KFCSQAEKDV LFTELQPQFL NLASNKYAVH FIQKMLDGAS
KQQLAACISS LRGHVAPLLR HVFGSLVVEH AYHLGSAAQK QELLAELYST ELQLFKGLTT
SNEKTVVDII AKLGLQKGAV NRHMTAIIQP ILEKGIVDHT ITHKLLIEYL TIADKTSAAD
VLQLLTGSLL LRMVHTRDGS RLAMLSIKHG SAKERKKIIK AMKEHVKKMA FDQFGSMVLA
CIFSIVDDTK LVTKIIVREL EATLKDLVMD KNGRRPLLQL LHPNSSRYLS HDDLAALDLS
VPSLCSMDKS ETSSKTKDTD GNEIGEETKD EQEDTVAEHS DHEENVTAMG GKKDPLVRRQ
ELLVNSGLAE RLIDVCVENA EEFLQSKFGN EVMYEVAIGG SDGILCPSLS EKLCELYEAI
SSVAAEPKPQ ESEKDSEHIL ENFHSSRTIR RLVLNRPGFA SILFKKALSG KCRSWAQGHC
SKILSAFVET EDVQVREMAK TELQVLVNEG TLKISATKKP E
