ID   PUM25_ARATH             Reviewed;         137 AA.
AC   Q9LK18;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 2.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Putative pumilio homolog 25;
DE            Short=APUM-25;
DE            Short=AtPUM25;
GN   Name=APUM25; OrderedLocusNames=At3g24270; ORFNames=K7M2.3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA   Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT   features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT   clones.";
RL   DNA Res. 7:217-221(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY.
RX   PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA   Francischini C.W., Quaggio R.B.;
RT   "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT   specificity and target candidates.";
RL   FEBS J. 276:5456-5470(2009).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA   Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT   "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT   modeling, activity and subcellular localization.";
RL   BMC Plant Biol. 10:44-44(2010).
CC   -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC       translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC       together to form a right-handed superhelix that approximates a half
CC       donut. The number as well as the specific sequence of the repeats
CC       determine the specificity for target mRNAs (By similarity).
CC       {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB02926.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP000382; BAB02926.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002686; AEE76883.1; -; Genomic_DNA.
DR   RefSeq; NP_189070.1; NM_113333.1.
DR   AlphaFoldDB; Q9LK18; -.
DR   SMR; Q9LK18; -.
DR   STRING; 3702.AT3G24270.1; -.
DR   PaxDb; Q9LK18; -.
DR   PRIDE; Q9LK18; -.
DR   EnsemblPlants; AT3G24270.1; AT3G24270.1; AT3G24270.
DR   GeneID; 822015; -.
DR   Gramene; AT3G24270.1; AT3G24270.1; AT3G24270.
DR   KEGG; ath:AT3G24270; -.
DR   Araport; AT3G24270; -.
DR   TAIR; locus:2087228; AT3G24270.
DR   HOGENOM; CLU_1867939_0_0_1; -.
DR   PRO; PR:Q9LK18; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LK18; baseline and differential.
DR   Genevisible; Q9LK18; AT.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   SUPFAM; SSF48371; SSF48371; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; Repeat; RNA-binding; Translation regulation.
FT   CHAIN           1..137
FT                   /note="Putative pumilio homolog 25"
FT                   /id="PRO_0000401407"
FT   REPEAT          70..105
FT                   /note="Pumilio 1"
FT   REPEAT          108..137
FT                   /note="Pumilio 2"
SQ   SEQUENCE   137 AA;  15798 MW;  9E3470C6B493766A CRC64;
     MVQLLFKFLI QVGATFVELS RNEIGNKNLL LLIHHAGSLL LSMFGSFSNY PVQKFLDMLD
     ERCLTLIASE FDSYFENLVK DRVGNYVVQR LIWGFKRTGI DLPHSLTSVL VTRSIHLCKH
     RYGYQVIEAF DRSTRLA