PUM2_ARATH
ID PUM2_ARATH Reviewed; 972 AA.
AC Q9ZW06; Q0WVV2; Q8LPN4;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Pumilio homolog 2;
DE Short=APUM-2;
DE Short=AtPUM2;
GN Name=APUM2; OrderedLocusNames=At2g29190; ORFNames=F16P2.43;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-736.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, FUNCTION, AND RNA-BINDING.
RX PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA Francischini C.W., Quaggio R.B.;
RT "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT specificity and target candidates.";
RL FEBS J. 276:5456-5470(2009).
RN [6]
RP GENE FAMILY, AND RNA-BINDING.
RX PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT modeling, activity and subcellular localization.";
RL BMC Plant Biol. 10:44-44(2010).
CC -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC Binds the APUM-binding elements (APBEs) in the 3'-UTR mRNA sequence of
CC CLV1, PNH, WUS and FAS2. {ECO:0000269|PubMed:19682068}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC together to form a right-handed superhelix that approximates a half
CC donut. The number as well as the specific sequence of the repeats
CC determine the specificity for target mRNAs (By similarity).
CC {ECO:0000250}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC004561; AAC95216.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08220.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08221.1; -; Genomic_DNA.
DR EMBL; AY096653; AAM20149.1; -; mRNA.
DR EMBL; AK226635; BAE98746.1; -; mRNA.
DR PIR; E84693; E84693.
DR RefSeq; NP_001189630.1; NM_001202701.1.
DR RefSeq; NP_180482.1; NM_128475.4.
DR AlphaFoldDB; Q9ZW06; -.
DR SMR; Q9ZW06; -.
DR BioGRID; 2818; 1.
DR STRING; 3702.AT2G29190.2; -.
DR iPTMnet; Q9ZW06; -.
DR PaxDb; Q9ZW06; -.
DR PRIDE; Q9ZW06; -.
DR ProteomicsDB; 226257; -.
DR EnsemblPlants; AT2G29190.1; AT2G29190.1; AT2G29190.
DR EnsemblPlants; AT2G29190.2; AT2G29190.2; AT2G29190.
DR GeneID; 817468; -.
DR Gramene; AT2G29190.1; AT2G29190.1; AT2G29190.
DR Gramene; AT2G29190.2; AT2G29190.2; AT2G29190.
DR KEGG; ath:AT2G29190; -.
DR Araport; AT2G29190; -.
DR TAIR; locus:2043187; AT2G29190.
DR eggNOG; KOG1488; Eukaryota.
DR HOGENOM; CLU_004017_1_1_1; -.
DR InParanoid; Q9ZW06; -.
DR OMA; MYHQYAD; -.
DR OrthoDB; 206600at2759; -.
DR PhylomeDB; Q9ZW06; -.
DR PRO; PR:Q9ZW06; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZW06; baseline and differential.
DR Genevisible; Q9ZW06; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IDA:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0003723; F:RNA binding; IDA:TAIR.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR CDD; cd07920; Pumilio; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR012940; NABP.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR033712; Pumilio_RNA-bd.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR Pfam; PF07990; NABP; 2.
DR Pfam; PF00806; PUF; 8.
DR SMART; SM00025; Pumilio; 8.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 9.
DR PROSITE; PS50303; PUM_HD; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome; Repeat; RNA-binding;
KW Translation regulation.
FT CHAIN 1..972
FT /note="Pumilio homolog 2"
FT /id="PRO_0000401384"
FT DOMAIN 614..954
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 634..669
FT /note="Pumilio 1"
FT REPEAT 670..705
FT /note="Pumilio 2"
FT REPEAT 706..741
FT /note="Pumilio 3"
FT REPEAT 742..777
FT /note="Pumilio 4"
FT REPEAT 778..814
FT /note="Pumilio 5"
FT REPEAT 815..850
FT /note="Pumilio 6"
FT REPEAT 851..886
FT /note="Pumilio 7"
FT REPEAT 887..928
FT /note="Pumilio 8"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 139..163
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 211..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 268..314
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..25
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 221..249
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 286..314
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 202
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9SS47"
FT MOD_RES 269
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9ZW02"
FT CONFLICT 441
FT /note="A -> T (in Ref. 3; AAM20149)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 972 AA; 106655 MW; 187AF6189E4CBAFB CRC64;
MIPELGRRPM HRGNEDSSFG DDYEKEIGVL LGEQQRRQVE ADELERELNL YRSGSAPPTV
DGSVSAAGGL FSGGGGAPFL EFGGGNKGNG FGGDDEEFRK DPAYLSYYYA NMKLNPRLPP
PLMSREDLRV AQRLKGSSNV LGGVGDRRKV NDSQSLFSMP PGFDQRKQHE FEVEKTSASS
SEWDANGLIG LPGLGIGGKQ KSFADIFQAD MGHGHPVTKQ PSRPASRNTF DENVDSKNNL
SPSASQGIGA PSPYSYAAVL GSSLSRNGTP DPQAIARVPS PCLTPIGSGR VSSNDKRNTS
NQSPFNGGLN ESSDLVNALS GMNLSGSGGL DERGQAEQDV EKVRNYMFGL QGGHNEVNQH
GFPNKSDQAQ KATGLLRNSQ LRGAQGSTYN DGGGVATQYQ HLDSPNYCLN NYGLNPAVAS
MMANQLGTNN YSPVYENASA ASAMGFSGMD SRLHGGGYVS SGQNLSESRN LGRFSNRMMG
GGTGLQSHMA DPMYHQYADS LDLLNDPSMD VNFMGNSYMN MLELQRAYLG AQKSQYGVPY
KSGSPNSHTD YGSPTFGSYP GSPLAHHLLP NSLVSPCSPM RRGEVNMRYP SATRNYAGGV
MGSWHMDASL DEGFGSSMLE EFKSNKTRGF ELAEIAGHVV EFSSDQYGSR FIQQKLETAT
SDEKNMVYEE IMPHALALMT DVFGNYVIQK FFEHGLPPQR RELADKLFDN VLPLSLQMYG
CRVIQKAIEV VDLDQKIKMV KELDGHVMRC VRDQNGNHVV QKCIECVPEE NIEFIISTFF
GNVVTLSTHP YGCRVIQRVL EHCHDPDTQS KVMDEIMSTI SMLAQDQYGN YVIQHVLEHG
KPDERTVIIK ELAGKIVQMS QQKFASNVVE KCLTFGGPEE REFLVNEMLG TTDENEPLQA
MMKDQFANYV VQKVLETCDD QQRELILGRI KVHLNALKKY TYGKHIVARV EKLVAAGERR
MALQSLTQPQ MA
