PUM3_MOUSE
ID PUM3_MOUSE Reviewed; 647 AA.
AC Q8BKS9; Q6A0E6; Q8BU15;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 2.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=Pumilio homolog 3 {ECO:0000305};
GN Name=Pum3 {ECO:0000312|MGI:MGI:106253};
GN Synonyms=D19Bwg1357e {ECO:0000312|MGI:MGI:106253},
GN Kiaa0020 {ECO:0000312|MGI:MGI:106253};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD;
RC TISSUE=Embryo, Embryonic spinal ganglion, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 152-647.
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=19319195; DOI=10.1371/journal.pone.0004980;
RA Kuo M.W., Wang S.H., Chang J.C., Chang C.H., Huang L.J., Lin H.H., Yu A.L.,
RA Li W.H., Yu J.;
RT "A novel puf-A gene predicted from evolutionary analysis is involved in the
RT development of eyes and primordial germ-cells.";
RL PLoS ONE 4:E4980-E4980(2009).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Pancreas, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-33, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
CC -!- FUNCTION: Inhibits the poly(ADP-ribosyl)ation activity of PARP1 and the
CC degradation of PARP1 by CASP3 following genotoxic stress. Binds to
CC double-stranded RNA or DNA without sequence specificity. Involved in
CC development of the eye and of primordial germ cells.
CC {ECO:0000250|UniProtKB:Q15397, ECO:0000250|UniProtKB:X1WGX5}.
CC -!- SUBUNIT: Interacts with PARP1 (via catalytic domain).
CC {ECO:0000250|UniProtKB:Q15397}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q15397}. Nucleus, nucleoplasm
CC {ECO:0000250|UniProtKB:Q15397}. Chromosome
CC {ECO:0000250|UniProtKB:Q15397}. Note=Localizes predominantly in the
CC nucleolus with minor punctate signals in the nucleoplasm.
CC {ECO:0000250|UniProtKB:Q15397}.
CC -!- TISSUE SPECIFICITY: In the adult eye, expressed primarily in retinal
CC ganglion cells and, to a lesser extent, in the pigmented cells.
CC {ECO:0000269|PubMed:19319195}.
CC -!- DOMAIN: A 90 degree bend between Pumilio repeats 3 and 4 gives rise to
CC a L-shaped protein. {ECO:0000250|UniProtKB:Q15397}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC34427.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC40135.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAE24873.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK050829; BAC34427.1; ALT_INIT; mRNA.
DR EMBL; AK088081; BAC40135.1; ALT_FRAME; mRNA.
DR EMBL; AK141893; BAE24873.1; ALT_INIT; mRNA.
DR EMBL; AK172872; BAD32150.1; -; mRNA.
DR RefSeq; NP_803425.1; NM_177474.5.
DR RefSeq; XP_006527246.1; XM_006527183.3.
DR AlphaFoldDB; Q8BKS9; -.
DR SMR; Q8BKS9; -.
DR BioGRID; 206863; 4.
DR STRING; 10090.ENSMUSP00000075573; -.
DR iPTMnet; Q8BKS9; -.
DR PhosphoSitePlus; Q8BKS9; -.
DR EPD; Q8BKS9; -.
DR MaxQB; Q8BKS9; -.
DR PaxDb; Q8BKS9; -.
DR PeptideAtlas; Q8BKS9; -.
DR PRIDE; Q8BKS9; -.
DR ProteomicsDB; 301926; -.
DR DNASU; 52874; -.
DR GeneID; 52874; -.
DR KEGG; mmu:52874; -.
DR UCSC; uc008hby.1; mouse.
DR CTD; 9933; -.
DR MGI; MGI:106253; Pum3.
DR eggNOG; KOG2050; Eukaryota.
DR InParanoid; Q8BKS9; -.
DR OMA; WILDDVY; -.
DR OrthoDB; 845051at2759; -.
DR PhylomeDB; Q8BKS9; -.
DR TreeFam; TF312954; -.
DR BioGRID-ORCS; 52874; 4 hits in 40 CRISPR screens.
DR ChiTaRS; Pum3; mouse.
DR PRO; PR:Q8BKS9; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q8BKS9; protein.
DR GO; GO:0005694; C:chromosome; ISS:UniProtKB.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0010835; P:regulation of protein ADP-ribosylation; ISS:UniProtKB.
DR GO; GO:0006417; P:regulation of translation; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR012959; CPL_dom.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR040059; PUM3.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR PANTHER; PTHR13389; PTHR13389; 1.
DR Pfam; PF08144; CPL; 1.
DR SMART; SM00025; Pumilio; 6.
DR SUPFAM; SSF48371; SSF48371; 2.
DR PROSITE; PS50302; PUM; 6.
DR PROSITE; PS50303; PUM_HD; 1.
PE 1: Evidence at protein level;
KW Acetylation; Chromosome; DNA-binding; Nucleus; Reference proteome; Repeat;
KW RNA-binding.
FT CHAIN 1..647
FT /note="Pumilio homolog 3"
FT /id="PRO_0000075930"
FT DOMAIN 142..509
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 176..211
FT /note="Pumilio 1"
FT /evidence="ECO:0000250|UniProtKB:Q15397"
FT REPEAT 212..247
FT /note="Pumilio 2"
FT /evidence="ECO:0000250|UniProtKB:Q15397"
FT REPEAT 248..276
FT /note="Pumilio 3"
FT /evidence="ECO:0000250|UniProtKB:Q15397"
FT REPEAT 288..324
FT /note="Pumilio 4"
FT /evidence="ECO:0000250|UniProtKB:Q15397"
FT REPEAT 325..360
FT /note="Pumilio 5"
FT /evidence="ECO:0000250|UniProtKB:Q15397"
FT REPEAT 361..396
FT /note="Pumilio 6"
FT /evidence="ECO:0000250|UniProtKB:Q15397"
FT REPEAT 397..434
FT /note="Pumilio 7"
FT /evidence="ECO:0000250|UniProtKB:Q15397"
FT REPEAT 435..503
FT /note="Pumilio 8"
FT /evidence="ECO:0000250|UniProtKB:Q15397"
FT REPEAT 504..550
FT /note="Pumilio 9"
FT /evidence="ECO:0000250|UniProtKB:Q15397"
FT REPEAT 551..595
FT /note="Pumilio 10"
FT /evidence="ECO:0000250|UniProtKB:Q15397"
FT REPEAT 596..635
FT /note="Pumilio 11"
FT /evidence="ECO:0000250|UniProtKB:Q15397"
FT REGION 1..123
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 105..117
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250|UniProtKB:Q15397"
FT COMPBIAS 10..36
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 72..123
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 33
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
SQ SEQUENCE 647 AA; 72800 MW; 193533640A3AFE50 CRC64;
MEVKGKKKFT GKSPQTSQGK NKFHKNSESS SSKTFPRKAV KEGGPKVTSK NFEKGATKPG
KKGVKQFKNK PQGGKGPKDK FQKANKFSKK RKFQPDGESD ESGAKKPKWD DFKKKKKELK
QSRQLSDKTN YDIVVRAKHI WESLRRKDCD KEKRVKLMSD LQKLIQGKIK TIAFAHDSTR
VIQCFIQYGN EEQRKQAFQE LQGDLVELSK AKYSRNIVKK FLMYGSKPQV AEIIRSFKGH
VRKMLRHSEA SAIVEYAYND KAILEQRNML TEELYGNTFQ LYKSADHPTL DKVLELQPAK
LELIMDEMKQ ILTPMAQKEA VIKHSLVHKV FLDFFTYAPP KPRSELIEAI REAVVYLAHT
HDGARVAMHC LWHGTPKDRK VIVKTMKTYV EKVANGQYSH LVLLAAFDCI DDTKLVKQII
ISEIISSLPS IVNDKYGRKV LLYLMSPRDP AHTVPELIEL LQKGDGNAHS KKDTAIRRRE
LLESISPALL SYLQGHTQEV VLDKSACVLV SDMLGSATGD VQPAMDAIAS LAAAELHPGG
KDGELHVAEH PAGHLVLKWL LEQDKKMKES GKEGCFAKTL VERVGMKNLK SWASINRGAI
ILSSLLQSCD QEVVNKVKGG LKPLIPTLEK NKSSSRGIQT LLEKLTA
