PUM7_ARATH
ID PUM7_ARATH Reviewed; 650 AA.
AC Q9C9R6;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Putative pumilio homolog 7, chloroplastic;
DE Short=APUM-7;
DE Short=AtPUM7;
DE Flags: Precursor;
GN Name=APUM7; OrderedLocusNames=At1g78160; ORFNames=T11I11.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA Francischini C.W., Quaggio R.B.;
RT "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT specificity and target candidates.";
RL FEBS J. 276:5456-5470(2009).
RN [4]
RP GENE FAMILY, AND SUBCELLULAR LOCATION.
RX PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT modeling, activity and subcellular localization.";
RL BMC Plant Biol. 10:44-44(2010).
CC -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}. Cytoplasm
CC {ECO:0000269|PubMed:20214804}.
CC -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC together to form a right-handed superhelix that approximates a half
CC donut. The number as well as the specific sequence of the repeats
CC determine the specificity for target mRNAs (By similarity).
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52093.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC012680; AAG52093.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE36075.1; -; Genomic_DNA.
DR PIR; H96810; H96810.
DR RefSeq; NP_177940.2; NM_106466.2.
DR AlphaFoldDB; Q9C9R6; -.
DR SMR; Q9C9R6; -.
DR STRING; 3702.AT1G78160.1; -.
DR PaxDb; Q9C9R6; -.
DR PRIDE; Q9C9R6; -.
DR EnsemblPlants; AT1G78160.1; AT1G78160.1; AT1G78160.
DR GeneID; 844152; -.
DR Gramene; AT1G78160.1; AT1G78160.1; AT1G78160.
DR KEGG; ath:AT1G78160; -.
DR Araport; AT1G78160; -.
DR TAIR; locus:2194699; AT1G78160.
DR eggNOG; KOG2049; Eukaryota.
DR HOGENOM; CLU_004017_5_1_1; -.
DR InParanoid; Q9C9R6; -.
DR PhylomeDB; Q9C9R6; -.
DR PRO; PR:Q9C9R6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C9R6; baseline and differential.
DR Genevisible; Q9C9R6; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR CDD; cd07920; Pumilio; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR033712; Pumilio_RNA-bd.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR Pfam; PF00806; PUF; 7.
DR SMART; SM00025; Pumilio; 8.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 8.
DR PROSITE; PS50303; PUM_HD; 1.
PE 3: Inferred from homology;
KW Chloroplast; Cytoplasm; Plastid; Reference proteome; Repeat; RNA-binding;
KW Transit peptide; Translation regulation.
FT TRANSIT 1..77
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 78..650
FT /note="Putative pumilio homolog 7, chloroplastic"
FT /id="PRO_0000401389"
FT DOMAIN 308..650
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 333..368
FT /note="Pumilio 1"
FT REPEAT 369..404
FT /note="Pumilio 2"
FT REPEAT 408..443
FT /note="Pumilio 3"
FT REPEAT 445..480
FT /note="Pumilio 4"
FT REPEAT 481..516
FT /note="Pumilio 5"
FT REPEAT 517..552
FT /note="Pumilio 6"
FT REPEAT 553..591
FT /note="Pumilio 7"
FT REPEAT 594..625
FT /note="Pumilio 8"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 200..235
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..22
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..234
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 650 AA; 73501 MW; E0A298DC46009040 CRC64;
MDEFREASSV SSSPSTPLRT PLHSPNLFNG DFSVSNRFSF KSSSDYSLSS SFSNGLCSPE
DSSSPFSSPP FNGIIPKHNH TSSSPVSFDS LFFKDHEKSH VNGTDDLGLC EDLYRMNIKE
DVEEDQIRYA RTETLKDPLP KSDHTDFTPD PLYNFSPKHY EPSNGGFVSG GFPYGFFRPP
KESSINQSCA SWSGFDQSKN DDKRNMFGNN PQQFGWPSYS SSNSGTSPYN NGQEIFENRG
GMREYSAYSP PHQPEVSYKH QNYRTTTSDI LPLFCQRTQV PMVSKCSEPF SSDESFFMNG
KSIDHQRSNT RALMSNNGNP TEICHPSLPN MCDIQGYVYL MAKDQHGCRF LQRIFDEGTS
VDAMIIFNEV IAHVVELMMD PFGNYLMQKL LDVCTEEQRT QIVLVATEEP GQLIRISLNA
YGTRVVQRLV ETIRSGKQIS LVKLALRPGF LDLIKDLNGN HVIQRCLQCL STEDNKFIFD
AATKFCTEIA THRHGCCVLQ KCIAYSMRQQ REKLIAEISR NSLLLAQDPF GNYAVQFVIE
LRIPSAVAMM LAQLKGHYVQ LSMQKFSSHM VERCLMHCPE SRPQIVRELV SVPHFDQLLQ
DPYANFVIQA ALAATKGPLH ASLVEVIRPH SILRNNPYCK RIFSRNLLKK
