PUM8_ARATH
ID PUM8_ARATH Reviewed; 515 AA.
AC Q9LM20;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Putative pumilio homolog 8, chloroplastic;
DE Short=APUM-8;
DE Short=AtPUM8;
DE Flags: Precursor;
GN Name=APUM8; OrderedLocusNames=At1g22240; ORFNames=F16L1.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA Francischini C.W., Quaggio R.B.;
RT "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT specificity and target candidates.";
RL FEBS J. 276:5456-5470(2009).
RN [4]
RP GENE FAMILY, AND SUBCELLULAR LOCATION.
RX PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT modeling, activity and subcellular localization.";
RL BMC Plant Biol. 10:44-44(2010).
CC -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}. Cytoplasm
CC {ECO:0000269|PubMed:20214804}.
CC -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC together to form a right-handed superhelix that approximates a half
CC donut. The number as well as the specific sequence of the repeats
CC determine the specificity for target mRNAs (By similarity).
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF87849.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC073942; AAF87849.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; AEE30215.1; -; Genomic_DNA.
DR PIR; B86355; B86355.
DR RefSeq; NP_173643.1; NM_102074.2.
DR AlphaFoldDB; Q9LM20; -.
DR SMR; Q9LM20; -.
DR STRING; 3702.AT1G22240.1; -.
DR PaxDb; Q9LM20; -.
DR PRIDE; Q9LM20; -.
DR ProteomicsDB; 224864; -.
DR EnsemblPlants; AT1G22240.1; AT1G22240.1; AT1G22240.
DR GeneID; 838829; -.
DR Gramene; AT1G22240.1; AT1G22240.1; AT1G22240.
DR KEGG; ath:AT1G22240; -.
DR Araport; AT1G22240; -.
DR TAIR; locus:2015036; AT1G22240.
DR eggNOG; KOG2049; Eukaryota.
DR HOGENOM; CLU_004017_5_0_1; -.
DR InParanoid; Q9LM20; -.
DR OMA; CINHAQG; -.
DR OrthoDB; 1364009at2759; -.
DR PhylomeDB; Q9LM20; -.
DR PRO; PR:Q9LM20; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LM20; baseline and differential.
DR Genevisible; Q9LM20; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR CDD; cd07920; Pumilio; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR033712; Pumilio_RNA-bd.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR Pfam; PF00806; PUF; 7.
DR SMART; SM00025; Pumilio; 8.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 8.
DR PROSITE; PS50303; PUM_HD; 1.
PE 3: Inferred from homology;
KW Chloroplast; Cytoplasm; Plastid; Reference proteome; Repeat; RNA-binding;
KW Transit peptide; Translation regulation.
FT TRANSIT 1..70
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 71..515
FT /note="Putative pumilio homolog 8, chloroplastic"
FT /id="PRO_0000401390"
FT DOMAIN 174..515
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 198..233
FT /note="Pumilio 1"
FT REPEAT 234..269
FT /note="Pumilio 2"
FT REPEAT 270..308
FT /note="Pumilio 3"
FT REPEAT 310..345
FT /note="Pumilio 4"
FT REPEAT 346..381
FT /note="Pumilio 5"
FT REPEAT 382..417
FT /note="Pumilio 6"
FT REPEAT 418..456
FT /note="Pumilio 7"
FT REPEAT 457..490
FT /note="Pumilio 8"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..28
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 515 AA; 58014 MW; 82469DECE0F723CE CRC64;
MMRGEFGEAS SLSRSPSSPL QTEPHPQSPK FYRNHYVFSG DHSSYNSSSR FCLRSPSDYS
LSSYFSNGLC SSEDGSSQFA SPPLDGLMTK YNFGGDDLGL CESFDLLNVG EEDKTHHQTQ
RSSFGNYEAN GDGGFRYYSG FGVDQKDHQI SNSWNLGMQS CNMNNYSVPV HGKSGVGALF
DHQGISSNPN ESLPKVSEFQ GYVYFMAKDQ HGCRFLQWIF EDGSALDALV IFSEVIPHVV
ELMMDPFGNY LMQKLLDVCN EEQRTQIILM VTSEPGQLIR ISLNAYGTRV VQRLVESIKT
RKQISLVKSA LRPGFLNLIR DLNGNHVIQR CLQCLSTEDN EFIFEDATKF CIDIATHRHG
CCVLQKCIAY SSGLQREKLV TEISRNSLFL AQDPYGNYAV QFVLELRDFS AIAAMLAQLK
GHYVELSMQK FSSHMVERCL THCPESRPQI VRELISVPHF DILIQDPYAN FVIQAALAVT
KGSLHATLVE VIRPHSILRN NPYCKRIFSR NLLKN
