PUM9_ARATH
ID PUM9_ARATH Reviewed; 564 AA.
AC Q1PFN9; Q9LP23;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Pumilio homolog 9;
DE Short=APUM-9;
DE Short=AtPUM9;
GN Name=APUM9; OrderedLocusNames=At1g35730; ORFNames=F14D7.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP GENE FAMILY.
RX PubMed=19682068; DOI=10.1111/j.1742-4658.2009.07230.x;
RA Francischini C.W., Quaggio R.B.;
RT "Molecular characterization of Arabidopsis thaliana PUF proteins -- binding
RT specificity and target candidates.";
RL FEBS J. 276:5456-5470(2009).
RN [5]
RP GENE FAMILY, AND SUBCELLULAR LOCATION.
RX PubMed=20214804; DOI=10.1186/1471-2229-10-44;
RA Tam P.P., Barrette-Ng I.H., Simon D.M., Tam M.W., Ang A.L., Muench D.G.;
RT "The Puf family of RNA-binding proteins in plants: phylogeny, structural
RT modeling, activity and subcellular localization.";
RL BMC Plant Biol. 10:44-44(2010).
CC -!- FUNCTION: Sequence-specific RNA-binding protein that regulates
CC translation and mRNA stability by binding the 3'-UTR of target mRNAs.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:20214804}.
CC -!- DOMAIN: The pumilio repeats mediate the association with RNA by packing
CC together to form a right-handed superhelix that approximates a half
CC donut. The number as well as the specific sequence of the repeats
CC determine the specificity for target mRNAs (By similarity).
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79883.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC021198; AAF79883.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; AEE31826.1; -; Genomic_DNA.
DR EMBL; DQ446324; ABE65687.1; -; mRNA.
DR PIR; D86479; D86479.
DR RefSeq; NP_174811.1; NM_103275.1.
DR AlphaFoldDB; Q1PFN9; -.
DR SMR; Q1PFN9; -.
DR STRING; 3702.AT1G35730.1; -.
DR iPTMnet; Q1PFN9; -.
DR PaxDb; Q1PFN9; -.
DR PRIDE; Q1PFN9; -.
DR EnsemblPlants; AT1G35730.1; AT1G35730.1; AT1G35730.
DR GeneID; 840477; -.
DR Gramene; AT1G35730.1; AT1G35730.1; AT1G35730.
DR KEGG; ath:AT1G35730; -.
DR Araport; AT1G35730; -.
DR TAIR; locus:2011349; AT1G35730.
DR eggNOG; KOG2049; Eukaryota.
DR HOGENOM; CLU_004017_5_2_1; -.
DR InParanoid; Q1PFN9; -.
DR OMA; FLEIINH; -.
DR PhylomeDB; Q1PFN9; -.
DR PRO; PR:Q1PFN9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q1PFN9; baseline and differential.
DR Genevisible; Q1PFN9; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:1902039; P:negative regulation of seed dormancy process; IMP:TAIR.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR CDD; cd07920; Pumilio; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033133; PUM-HD.
DR InterPro; IPR033712; Pumilio_RNA-bd.
DR InterPro; IPR001313; Pumilio_RNA-bd_rpt.
DR Pfam; PF00806; PUF; 8.
DR SMART; SM00025; Pumilio; 8.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50302; PUM; 7.
DR PROSITE; PS50303; PUM_HD; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Reference proteome; Repeat; RNA-binding; Translation regulation.
FT CHAIN 1..564
FT /note="Pumilio homolog 9"
FT /id="PRO_0000401391"
FT DOMAIN 222..564
FT /note="PUM-HD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00318"
FT REPEAT 249..284
FT /note="Pumilio 1"
FT REPEAT 285..320
FT /note="Pumilio 2"
FT REPEAT 321..359
FT /note="Pumilio 3"
FT REPEAT 361..396
FT /note="Pumilio 4"
FT REPEAT 397..432
FT /note="Pumilio 5"
FT REPEAT 433..469
FT /note="Pumilio 6"
FT REPEAT 470..501
FT /note="Pumilio 7"
FT REPEAT 502..539
FT /note="Pumilio 8"
SQ SEQUENCE 564 AA; 63251 MW; 879C475D3FC5EE2B CRC64;
MGFGGFNGDF NFRGASDHRP LGSDGFLPSL DTNPFLKNHK SVEALDLCKK LHKMGISCDM
SIWTKPEEQF RVDPSEFGAR TLHGSSGFDQ NLTGASQIHD GFRNFSSVRL QNNNFHGVSP
SPGEMRLLGR QDSFNLNGFE EMLALKNHRD FLLDQIHEPI KRPPFLRGND ALISSLMFEG
NNRVSQTLAA MEASRGFYPE EDSSLLSPFH EKVSGKLGAS YLEDTVLIGQ GSYGKMSPKS
NNDLVSMVEI YGSVNLMAKD QIGCRVLQKL VEEGTFHEAK VILLAIIDHV VELSMDPFGN
YIVQKLFDVS DEEQRTLIVS VLTSNPRELI RICLNTYGTR VVQKMIETVK TKQQIALVKS
GLKPGFLALV KDLNGNHVIQ SCLQTLGPND NEFVLEAATK YCAEIAIHRH GCCVLQCCIS
NSVGLQRERL VAEISRNSLH LSQDPFGNYV VQYLIDQQVS AVKLLVQFRM HYAELATQKF
SSHVIEKCLR KYPESRAEIV RELLCVPNFE YLLQDPYANY VIQTALSVTK GPVRAKLVAK
VYRYGKLHSS PYCKKIFSKT ILKK
