ID   PUMA_PARUN              Reviewed;        1955 AA.
AC   O61308;
DT   25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=227 kDa spindle- and centromere-associated protein;
DE   AltName: Full=PUMA1;
GN   Name=PUMA1;
OS   Parascaris univalens (Nematode worm).
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Spirurina; Ascaridomorpha; Ascaridoidea; Ascarididae; Parascaris.
OX   NCBI_TaxID=6257;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Embryo;
RX   PubMed=9472001; DOI=10.1242/jcs.111.6.723;
RA   Esteban M.R., Giovinazzo G., de la Hera A., Goday C.;
RT   "PUMA1: a novel protein that associates with the centrosomes, spindle and
RT   centromeres in the nematode Parascaris.";
RL   J. Cell Sci. 111:723-735(1998).
CC   -!- FUNCTION: May play a role in the organization of the spindle apparatus
CC       and its interaction with the centromeres.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC       center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm,
CC       cytoskeleton, spindle. Chromosome, centromere. Note=Centrosomes and
CC       kinetochore microtubules of mitotic cells. Also localizes to the
CC       spindle mid- zone region during anaphase and to the midbody during
CC       telophase. During meiosis, associates with centrosomes and discrete
CC       centromeric regions lacking kinetochore structures.
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DR   EMBL; AF009623; AAC38995.1; -; mRNA.
DR   PIR; T30934; T30934.
DR   AlphaFoldDB; O61308; -.
DR   SMR; O61308; -.
DR   PRIDE; O61308; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR   GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR   GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Cytoplasm;
KW   Cytoskeleton; Kinetochore; Meiosis; Mitosis.
FT   CHAIN           1..1955
FT                   /note="227 kDa spindle- and centromere-associated protein"
FT                   /id="PRO_0000097106"
FT   REGION          1865..1896
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1912..1955
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          82..129
FT                   /evidence="ECO:0000255"
FT   COILED          152..317
FT                   /evidence="ECO:0000255"
FT   COILED          385..1747
FT                   /evidence="ECO:0000255"
FT   COILED          1770..1813
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1875..1896
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1955 AA;  227218 MW;  495B8F92A5C0A111 CRC64;
     MEGTCSEEEN LAKRVTPTAV TSGTYGTHVV DTTDLALSDV VIGCGSTIYE TSDSIGSDVM
     LGLSSHGVSS VSGLDRDLNS FKKRIDANTE EQREHADMMV GLQRKIEEYR RRIVDAEKQV
     SIQKANDDVS FSIKETADTW LPDMKSDAAD YEWASRLDEE RRRNDELHMQ ITQQQVDLQR
     LQKYFEANMQ EKEKIYQTRE KNLAYYLNAE QRKMLDLWEE LQRVRRQFAD YKEQTERDLE
     NQKNEVAKVT QSVGGMAGRL NTSSHGDSGL VQDVVLLEAM RRFRELQAVP VGASADDYNA
     LMKKYEETVE RVIELESHGD GSTAKMLSLE AELRRTKDKF IECSEFLRKL GDLAAGSYRG
     DERTSKIVSL SPGGMSLPSE IYRSVRNILR NHDSEMQHIQ RKLKNSDTQV CELTTRLEGT
     EEARRRSDKQ LVDAKREINI QQRAVDDANR ELRRVEDRLH IMESEKIVAE NARQQLEEEV
     RRLTLQVDQS KADGERRVVE EGEIQKRIVE DEYRSMISEL TRRMNAFQDE NKRLKNDLGC
     TKERLKNVEF EYNSTVRKLE DKDIALKHLE DTKLDLLKDL ENQRTRYDAV TNELDTLQTT
     FETSTKNIAQ LEANIKEINL MRDEISKEKD SLAQKLADVT HKLEIETVRR EDIQRSCVGH
     SEDVEKQRLQ IIEYEREVMA LRRLNDELDT NVKTGQAKVT SLENSIISVQ TEVTKLTTLN
     DKLQKEKQSI MSSKQKADTD VDLLKEKLRK LEQECDKLKE ENKALHEDEQ IARQMCKEEA
     SRIHLLERDL KDAMTEVEEL KKQLQKMDEE NSERLESVLR TKISSDTVDT SEIAEYTEVK
     VKELREKYKA DLERLQSNKD DLERRVQILE DELAERQRIV ERQRTEMNDL KLEYQLESDR
     LRAEMATVEL KYQSEVEDER DQRSRDADSW KVTSEELRSK ISFMEKMLEE AKHRETVLRE
     EATEWEEKHD IISNESLKLR NEIERIRSDA EEDIQKWKKD VHMAQNELKN LERVCETLRS
     QLTAANDRVA SLNTTINEQT SKIRELNSHE HRLEEDLADS RATSSAIEND LGNATGRLRS
     SEEHNAILQS ENRKSKTEIE ALKHQIDTIM NTKESCESEV ERLKKKIVQT TTITKEQNEK
     IEKLRIEHDH LERDYREKTK EVDRLKEVEK TFELKVNRAR QELDEFSKKL IVTETERNAI
     SGEAQKLDKE VQLVKEQLQY KSDEFHKALD ELANAHRISE EGRVNAIHQL EARRFEIDDL
     KSRLENSEQR LATLQQEYVN ADKERGALND AMRRFQATIS RSVVAEEHVD VSTIETQMQK
     LMSRIEAIER ERNEYRDSLN RLKNRCSTSY SSVDRQETVY RTFEERVISA EDERRKVELK
     LSSMKEMLKS QEEKLKQRDE ERRNLKSNIV TFELEARAKD AQIRHLNDLL KRVQAELENS
     QNDNRALRER QEQYETNRIH LEQRLPTDEG EPRVKALMAA FATERQSLSD SLKKLASQLQ
     ISETKNADLR DDAERLKRDL LKAERVEEDL RRNLVEQTEI MRENQQLRSQ LGVAQSDLAN
     ASGRKQQLEG ELAAVRAELR DHKQHLHDAI SRIAELQRQL QDANAEKSRL TDRIIGLEKT
     IGTLRNTETE LRAQLSTAAD ERKALNSELE EMRRRIVQME SEKKDVDNQL EEVNKARIIM
     TKKIEILETE KHSAELVISE TASQREAIER SLNALERENK ELYKNCAQLQ QQIAQLEMDN
     GERLIALTTK QKEDHEKFVA AVKAEKVQVE RIVENRDRAQ KSRIRQLENQ LSMMREQLDN
     ERARSHQMSE RFIVNETNRR VSSSSFRLSG DAAGVAAATI LHPQTDRLDY VFANRSALSS
     YYTVPTEQHA SRGKEAYRTS STIKSSEGTT RESYTYQSRT VSSNIIEQAN GMTSSASGEG
     MSRAYPPTEV NQGDVTGRKS RPATRKQQMK STFSE