PUMP1_ARATH
ID PUMP1_ARATH Reviewed; 306 AA.
AC O81845; O65623; Q56Z57;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Mitochondrial uncoupling protein 1;
DE Short=AtPUMP1;
GN Name=PUMP1; Synonyms=UCP1; OrderedLocusNames=At3g54110; ORFNames=F24B22.70;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION BY COLD.
RC STRAIN=cv. Columbia;
RX PubMed=9662458; DOI=10.1016/s0014-5793(98)00634-6;
RA Maia I.G., Benedetti C.E., Leite A., Turcinelli S.R., Vercesi A.E.,
RA Arruda P.;
RT "AtPUMP: an Arabidopsis gene encoding a plant uncoupling mitochondrial
RT protein.";
RL FEBS Lett. 429:403-406(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RA Laloi M., Klein M., Mueller-Roeber B., Willmitzer L.;
RT "Arabidopsis thaliana mitochondrial uncoupling protein.";
RL Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Dombrowski H.D., Cousins S.K., Harris M.A., Hanson M.R.;
RT "Expression of a plant mitochondrial uncoupling protein in yeast.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 123-306.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP FUNCTION.
RX PubMed=11566183; DOI=10.1016/s0014-5793(01)02835-6;
RA Borecky J., Maia I.G., Costa A.D., Jezek P., Chaimovich H.,
RA de Andrade P.B., Vercesi A.E., Arruda P.;
RT "Functional reconstitution of Arabidopsis thaliana plant uncoupling
RT mitochondrial protein (AtPUMP1) expressed in Escherichia coli.";
RL FEBS Lett. 505:240-244(2001).
RN [9]
RP FUNCTION.
RX PubMed=13678271; DOI=10.1023/a:1024603530043;
RA Brandalise M., Maia I.G., Borecky J., Vercesi A.E., Arruda P.;
RT "Overexpression of plant uncoupling mitochondrial protein in transgenic
RT tobacco increases tolerance to oxidative stress.";
RL J. Bioenerg. Biomembr. 35:203-209(2003).
RN [10]
RP INDUCTION BY CHLORAMPHENICOL.
RX PubMed=16027974; DOI=10.1007/s11103-005-5514-7;
RA Clifton R., Lister R., Parker K.L., Sappl P.G., Elhafez D., Millar A.H.,
RA Day D.A., Whelan J.;
RT "Stress-induced co-expression of alternative respiratory chain components
RT in Arabidopsis thaliana.";
RL Plant Mol. Biol. 58:193-212(2005).
RN [11]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16473895; DOI=10.1093/jxb/erj070;
RA Borecky J., Nogueira F.T., de Oliveira K.A., Maia I.G., Vercesi A.E.,
RA Arruda P.;
RT "The plant energy-dissipating mitochondrial systems: depicting the genomic
RT structure and the expression profiles of the gene families of uncoupling
RT protein and alternative oxidase in monocots and dicots.";
RL J. Exp. Bot. 57:849-864(2006).
RN [12]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=17148605; DOI=10.1073/pnas.0607751103;
RA Sweetlove L.J., Lytovchenko A., Morgan M., Nunes-Nesi A., Taylor N.L.,
RA Baxter C.J., Eickmeier I., Fernie A.R.;
RT "Mitochondrial uncoupling protein is required for efficient
RT photosynthesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:19587-19592(2006).
RN [13]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND MUTAGENESIS OF CYS-28; HIS-83;
RP LYS-147; ARG-155 AND TYR-269.
RX PubMed=17980348; DOI=10.1016/j.bbabio.2007.09.005;
RA Favaro R.D., Borecky J., Colombi D., Vercesi A.E., Maia I.G.;
RT "Mutational analysis of Arabidopsis thaliana plant uncoupling mitochondrial
RT protein.";
RL Biochim. Biophys. Acta 1767:1412-1417(2007).
RN [14]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=17272265; DOI=10.1074/mcp.m600408-mcp200;
RA Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.;
RT "Multidimensional protein identification technology (MudPIT) analysis of
RT ubiquitinated proteins in plants.";
RL Mol. Cell. Proteomics 6:601-610(2007).
RN [15]
RP INDUCTION BY HIGH LIGHT.
RX PubMed=19567377; DOI=10.1093/pcp/pcp090;
RA Yoshida K., Noguchi K.;
RT "Differential gene expression profiles of the mitochondrial respiratory
RT components in illuminated Arabidopsis leaves.";
RL Plant Cell Physiol. 50:1449-1462(2009).
RN [16]
RP FUNCTION.
RX PubMed=19875678; DOI=10.1093/pcp/pcp144;
RA Hourton-Cabassa C., Matos A.R., Arrabaca J., Demandre C., Zachowski A.,
RA Moreau F.;
RT "Genetically modified Arabidopsis thaliana cells reveal the involvement of
RT the mitochondrial fatty acid composition in membrane basal and uncoupling
RT protein-mediated proton leaks.";
RL Plant Cell Physiol. 50:2084-2091(2009).
RN [17]
RP FUNCTION.
RX PubMed=21912606; DOI=10.1371/journal.pone.0023776;
RA Begcy K., Mariano E.D., Mattiello L., Nunes A.V., Mazzafera P., Maia I.G.,
RA Menossi M.;
RT "An Arabidopsis mitochondrial uncoupling protein confers tolerance to
RT drought and salt stress in transgenic tobacco plants.";
RL PLoS ONE 6:E23776-E23776(2011).
CC -!- FUNCTION: PUMPS are mitochondrial transporter proteins that create
CC proton leaks across the inner mitochondrial membrane, thus uncoupling
CC oxidative phosphorylation. This leads to a decrease in the efficiency
CC of oxidative phosphorylation and an increase in heat production. Is
CC involved in protecting plant cells against oxidative stress damage and
CC maintaining the redox balance of the mitochondrial electron transport
CC chain to facilitate photosynthetic metabolism. May play a regulatory
CC role during photorespiration. {ECO:0000269|PubMed:11566183,
CC ECO:0000269|PubMed:13678271, ECO:0000269|PubMed:17148605,
CC ECO:0000269|PubMed:17980348, ECO:0000269|PubMed:19875678,
CC ECO:0000269|PubMed:21912606}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:17148605,
CC ECO:0000269|PubMed:9662458}.
CC -!- INDUCTION: By cold stress, high light and chloramphenicol.
CC {ECO:0000269|PubMed:16027974, ECO:0000269|PubMed:19567377,
CC ECO:0000269|PubMed:9662458}.
CC -!- DISRUPTION PHENOTYPE: Slight reduction of shoot mass.
CC {ECO:0000269|PubMed:17148605}.
CC -!- MISCELLANEOUS: Over-expression of Arabidopsis UCP1 in tobacco plants
CC increases tolerance to oxidative stress caused by exogenous hydrogen
CC peroxide, or by drought and salt. {ECO:0000305|PubMed:13678271}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD95028.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AJ223983; CAA11757.1; -; mRNA.
DR EMBL; AJ001264; CAA04638.1; -; mRNA.
DR EMBL; Y18291; CAA77109.1; -; Genomic_DNA.
DR EMBL; AF146226; AAF66705.1; -; mRNA.
DR EMBL; AL132957; CAB70985.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79189.1; -; Genomic_DNA.
DR EMBL; AY056272; AAL07121.1; -; mRNA.
DR EMBL; AY091185; AAM14124.1; -; mRNA.
DR EMBL; AK221112; BAD95028.1; ALT_INIT; mRNA.
DR PIR; T47570; T47570.
DR PIR; T52024; T52024.
DR RefSeq; NP_190979.1; NM_115271.5.
DR AlphaFoldDB; O81845; -.
DR BioGRID; 9895; 1.
DR STRING; 3702.AT3G54110.1; -.
DR TCDB; 2.A.29.3.3; the mitochondrial carrier (mc) family.
DR PaxDb; O81845; -.
DR PRIDE; O81845; -.
DR ProteomicsDB; 226359; -.
DR EnsemblPlants; AT3G54110.1; AT3G54110.1; AT3G54110.
DR GeneID; 824578; -.
DR Gramene; AT3G54110.1; AT3G54110.1; AT3G54110.
DR KEGG; ath:AT3G54110; -.
DR Araport; AT3G54110; -.
DR TAIR; locus:2080300; AT3G54110.
DR eggNOG; KOG0753; Eukaryota.
DR HOGENOM; CLU_015166_14_2_1; -.
DR InParanoid; O81845; -.
DR OMA; MMAANHS; -.
DR OrthoDB; 984118at2759; -.
DR PhylomeDB; O81845; -.
DR PRO; PR:O81845; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; O81845; baseline and differential.
DR Genevisible; O81845; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0017077; F:oxidative phosphorylation uncoupler activity; IDA:TAIR.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0003333; P:amino acid transmembrane transport; IDA:TAIR.
DR GO; GO:1990542; P:mitochondrial transmembrane transport; IBA:GO_Central.
DR GO; GO:0006839; P:mitochondrial transport; IEA:InterPro.
DR GO; GO:0009853; P:photorespiration; IMP:TAIR.
DR GO; GO:0015979; P:photosynthesis; IMP:TAIR.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR002030; Mit_uncoupling_UCP-like.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00784; MTUNCOUPLING.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Repeat; Stress response; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..306
FT /note="Mitochondrial uncoupling protein 1"
FT /id="PRO_0000420255"
FT TRANSMEM 15..35
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 9..102
FT /note="Solcar 1"
FT REPEAT 112..203
FT /note="Solcar 2"
FT REPEAT 212..296
FT /note="Solcar 3"
FT MUTAGEN 28
FT /note="C->A: Reduces proton transport activity 3-fold."
FT /evidence="ECO:0000269|PubMed:17980348"
FT MUTAGEN 83
FT /note="H->L: Reduces proton transport activity 3-fold."
FT /evidence="ECO:0000269|PubMed:17980348"
FT MUTAGEN 147
FT /note="K->H: Reduces proton transport activity 3-fold."
FT /evidence="ECO:0000269|PubMed:17980348"
FT MUTAGEN 155
FT /note="R->L: Slightly reduces proton transport activity."
FT /evidence="ECO:0000269|PubMed:17980348"
FT MUTAGEN 269
FT /note="Y->F: Reduces proton transport activity 3-fold."
FT /evidence="ECO:0000269|PubMed:17980348"
FT CONFLICT 178
FT /note="G -> V (in Ref. 1; CAA11757)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 306 AA; 32662 MW; ACCCB46F42442142 CRC64;
MVAAGKSDLS LPKTFACSAF AACVGEVCTI PLDTAKVRLQ LQKSALAGDV TLPKYRGLLG
TVGTIAREEG LRSLWKGVVP GLHRQCLFGG LRIGMYEPVK NLYVGKDFVG DVPLSKKILA
GLTTGALGIM VANPTDLVKV RLQAEGKLAA GAPRRYSGAL NAYSTIVRQE GVRALWTGLG
PNVARNAIIN AAELASYDQV KETILKIPGF TDNVVTHILS GLGAGFFAVC IGSPVDVVKS
RMMGDSGAYK GTIDCFVKTL KSDGPMAFYK GFIPNFGRLG SWNVIMFLTL EQAKKYVREL
DASKRN
