PUMP2_ARATH
ID PUMP2_ARATH Reviewed; 305 AA.
AC Q9ZWG1; F4KGF2;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Mitochondrial uncoupling protein 2;
DE Short=AtPUMP2;
GN Name=PUMP2; Synonyms=UCP2; OrderedLocusNames=At5g58970; ORFNames=K19M22.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia; TISSUE=Leaf;
RX PubMed=10635118; DOI=10.1093/oxfordjournals.pcp.a029501;
RA Watanabe A., Nakazono M., Tsutsumi N., Hirai A.;
RT "AtUCP2: a novel isoform of the mitochondrial uncoupling protein of
RT Arabidopsis thaliana.";
RL Plant Cell Physiol. 40:1160-1166(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Landsberg erecta;
RX PubMed=22430844; DOI=10.1104/pp.111.193151;
RA Parsons H.T., Christiansen K., Knierim B., Carroll A., Ito J., Batth T.S.,
RA Smith-Moritz A.M., Morrison S., McInerney P., Hadi M.Z., Auer M.,
RA Mukhopadhyay A., Petzold C.J., Scheller H.V., Loque D., Heazlewood J.L.;
RT "Isolation and proteomic characterization of the Arabidopsis Golgi defines
RT functional and novel components involved in plant cell wall biosynthesis.";
RL Plant Physiol. 159:12-26(2012).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP INDUCTION BY CHLORAMPHENICOL.
RX PubMed=16027974; DOI=10.1007/s11103-005-5514-7;
RA Clifton R., Lister R., Parker K.L., Sappl P.G., Elhafez D., Millar A.H.,
RA Day D.A., Whelan J.;
RT "Stress-induced co-expression of alternative respiratory chain components
RT in Arabidopsis thaliana.";
RL Plant Mol. Biol. 58:193-212(2005).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16473895; DOI=10.1093/jxb/erj070;
RA Borecky J., Nogueira F.T., de Oliveira K.A., Maia I.G., Vercesi A.E.,
RA Arruda P.;
RT "The plant energy-dissipating mitochondrial systems: depicting the genomic
RT structure and the expression profiles of the gene families of uncoupling
RT protein and alternative oxidase in monocots and dicots.";
RL J. Exp. Bot. 57:849-864(2006).
CC -!- FUNCTION: PUMPS are mitochondrial transporter proteins that create
CC proton leaks across the inner mitochondrial membrane, thus uncoupling
CC oxidative phosphorylation. This leads to a decrease in the efficiency
CC of oxidative phosphorylation and an increase in heat production. May be
CC involved in protecting plant cells against oxidative stress damage (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9ZWG1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9ZWG1-2; Sequence=VSP_044433, VSP_044434;
CC -!- INDUCTION: By chloramphenicol. {ECO:0000269|PubMed:16027974}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; AB021706; BAA36222.1; -; mRNA.
DR EMBL; JQ937241; AFI41209.1; -; mRNA.
DR EMBL; AB016885; BAB09640.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97124.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97125.1; -; Genomic_DNA.
DR EMBL; AY088200; AAM65742.1; -; mRNA.
DR EMBL; BX831274; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_568894.1; NM_125287.5. [Q9ZWG1-1]
DR RefSeq; NP_974962.1; NM_203233.1. [Q9ZWG1-2]
DR AlphaFoldDB; Q9ZWG1; -.
DR SMR; Q9ZWG1; -.
DR STRING; 3702.AT5G58970.1; -.
DR TCDB; 2.A.29.3.8; the mitochondrial carrier (mc) family.
DR PaxDb; Q9ZWG1; -.
DR PRIDE; Q9ZWG1; -.
DR ProteomicsDB; 226124; -. [Q9ZWG1-1]
DR EnsemblPlants; AT5G58970.1; AT5G58970.1; AT5G58970. [Q9ZWG1-1]
DR EnsemblPlants; AT5G58970.2; AT5G58970.2; AT5G58970. [Q9ZWG1-2]
DR GeneID; 836014; -.
DR Gramene; AT5G58970.1; AT5G58970.1; AT5G58970. [Q9ZWG1-1]
DR Gramene; AT5G58970.2; AT5G58970.2; AT5G58970. [Q9ZWG1-2]
DR KEGG; ath:AT5G58970; -.
DR Araport; AT5G58970; -.
DR TAIR; locus:2154593; AT5G58970.
DR eggNOG; KOG0753; Eukaryota.
DR HOGENOM; CLU_015166_14_2_1; -.
DR InParanoid; Q9ZWG1; -.
DR OMA; MMVTKKR; -.
DR OrthoDB; 984118at2759; -.
DR PhylomeDB; Q9ZWG1; -.
DR PRO; PR:Q9ZWG1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9ZWG1; baseline and differential.
DR Genevisible; Q9ZWG1; AT.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0017077; F:oxidative phosphorylation uncoupler activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0003333; P:amino acid transmembrane transport; IDA:TAIR.
DR GO; GO:1990542; P:mitochondrial transmembrane transport; IBA:GO_Central.
DR GO; GO:0006839; P:mitochondrial transport; IEA:InterPro.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR002030; Mit_uncoupling_UCP-like.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00784; MTUNCOUPLING.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Repeat; Stress response;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..305
FT /note="Mitochondrial uncoupling protein 2"
FT /id="PRO_0000420256"
FT TRANSMEM 16..36
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 10..104
FT /note="Solcar 1"
FT REPEAT 114..205
FT /note="Solcar 2"
FT REPEAT 214..297
FT /note="Solcar 3"
FT VAR_SEQ 241..272
FT /note="KSRMMGDSTYRNTVDCFIKTMKTEGIMAFYKG -> SIHFRLLHKSTTRLFG
FT FRLIMCGSAYIYNKFQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14993207"
FT /id="VSP_044433"
FT VAR_SEQ 273..305
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14993207"
FT /id="VSP_044434"
SQ SEQUENCE 305 AA; 33442 MW; B0F6428F758EDCD7 CRC64;
MADFKPRIEI SFLETFICSA FAACFAELCT IPLDTAKVRL QLQRKIPTGD GENLPKYRGS
IGTLATIARE EGISGLWKGV IAGLHRQCIY GGLRIGLYEP VKTLLVGSDF IGDIPLYQKI
LAALLTGAIA IIVANPTDLV KVRLQSEGKL PAGVPRRYAG AVDAYFTIVK LEGVSALWTG
LGPNIARNAI VNAAELASYD QIKETIMKIP FFRDSVLTHL LAGLAAGFFA VCIGSPIDVV
KSRMMGDSTY RNTVDCFIKT MKTEGIMAFY KGFLPNFTRL GTWNAIMFLT LEQVKKVFLR
EVLYD
