PUMP3_ARATH
ID PUMP3_ARATH Reviewed; 305 AA.
AC Q9XI74;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Mitochondrial uncoupling protein 3;
DE Short=AtPUMP3;
GN Name=PUMP3; Synonyms=UCP3; OrderedLocusNames=At1g14140; ORFNames=F7A19.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16473895; DOI=10.1093/jxb/erj070;
RA Borecky J., Nogueira F.T., de Oliveira K.A., Maia I.G., Vercesi A.E.,
RA Arruda P.;
RT "The plant energy-dissipating mitochondrial systems: depicting the genomic
RT structure and the expression profiles of the gene families of uncoupling
RT protein and alternative oxidase in monocots and dicots.";
RL J. Exp. Bot. 57:849-864(2006).
RN [6]
RP INDUCTION BY HIGH LIGHT.
RX PubMed=19567377; DOI=10.1093/pcp/pcp090;
RA Yoshida K., Noguchi K.;
RT "Differential gene expression profiles of the mitochondrial respiratory
RT components in illuminated Arabidopsis leaves.";
RL Plant Cell Physiol. 50:1449-1462(2009).
CC -!- FUNCTION: PUMPS are mitochondrial transporter proteins that create
CC proton leaks across the inner mitochondrial membrane, thus uncoupling
CC oxidative phosphorylation. This leads to a decrease in the efficiency
CC of oxidative phosphorylation and an increase in heat production. May be
CC involved in protecting plant cells against oxidative stress damage (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- INDUCTION: By high light. {ECO:0000269|PubMed:19567377}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; AC007576; AAD39300.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29110.1; -; Genomic_DNA.
DR EMBL; BT026111; ABG48467.1; -; mRNA.
DR EMBL; AY084432; AAM61005.1; -; mRNA.
DR PIR; H86274; H86274.
DR RefSeq; NP_172866.1; NM_101279.2.
DR AlphaFoldDB; Q9XI74; -.
DR SMR; Q9XI74; -.
DR STRING; 3702.AT1G14140.1; -.
DR PaxDb; Q9XI74; -.
DR PRIDE; Q9XI74; -.
DR ProteomicsDB; 226360; -.
DR EnsemblPlants; AT1G14140.1; AT1G14140.1; AT1G14140.
DR GeneID; 837973; -.
DR Gramene; AT1G14140.1; AT1G14140.1; AT1G14140.
DR KEGG; ath:AT1G14140; -.
DR Araport; AT1G14140; -.
DR TAIR; locus:2035769; AT1G14140.
DR eggNOG; KOG0753; Eukaryota.
DR HOGENOM; CLU_015166_14_2_1; -.
DR InParanoid; Q9XI74; -.
DR OMA; QLRPVKY; -.
DR OrthoDB; 1013743at2759; -.
DR PhylomeDB; Q9XI74; -.
DR PRO; PR:Q9XI74; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9XI74; baseline and differential.
DR Genevisible; Q9XI74; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006839; P:mitochondrial transport; IEA:InterPro.
DR GO; GO:0009409; P:response to cold; IBA:GO_Central.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR002030; Mit_uncoupling_UCP-like.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00784; MTUNCOUPLING.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 2: Evidence at transcript level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Repeat; Stress response; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..305
FT /note="Mitochondrial uncoupling protein 3"
FT /id="PRO_0000420257"
FT TRANSMEM 16..36
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 14..100
FT /note="Solcar 1"
FT REPEAT 112..204
FT /note="Solcar 2"
FT REPEAT 213..299
FT /note="Solcar 3"
SQ SEQUENCE 305 AA; 33441 MW; C1122C9B866BC32A CRC64;
MERSRVTREA PTGTRILLAS LSAMVAESVT FPIDLTKTRM QLHGSGSASG AHRIGAFGVV
SEIARKEGVI GLYKGLSPAI IRHLFYTPIR IIGYENLKGL IVRSETNNSE SLPLATKALV
GGFSGVIAQV VASPADLVKV RMQADGRLVS QGLKPRYSGP IEAFTKILQS EGVKGLWKGV
LPNIQRAFLV NMGELACYDH AKHFVIDKKI AEDNIFAHTL ASIMSGLAST SLSCPADVVK
TRMMNQGENA VYRNSYDCLV KTVKFEGIRA LWKGFFPTWA RLGPWQFVFW VSYEKFRLLA
GISSF
