PUMP6_ARATH
ID PUMP6_ARATH Reviewed; 337 AA.
AC Q9FY68;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Mitochondrial uncoupling protein 6;
DE Short=AtPUMP6;
DE AltName: Full=Mitochondrial dicarboxylate carrier 3;
GN Name=PUMP6; Synonyms=DIC3, UCP6; OrderedLocusNames=At5g09470;
GN ORFNames=T5E8.270;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=cv. Columbia; TISSUE=Flower bud;
RX PubMed=18039180; DOI=10.1042/bj20070867;
RA Palmieri L., Picault N., Arrigoni R., Besin E., Palmieri F., Hodges M.;
RT "Molecular identification of three Arabidopsis thaliana mitochondrial
RT dicarboxylate carrier isoforms: organ distribution, bacterial expression,
RT reconstitution into liposomes and functional characterization.";
RL Biochem. J. 410:621-629(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA De Los Reyes C., Quan R., Chen H., Bautista V.R., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16473895; DOI=10.1093/jxb/erj070;
RA Borecky J., Nogueira F.T., de Oliveira K.A., Maia I.G., Vercesi A.E.,
RA Arruda P.;
RT "The plant energy-dissipating mitochondrial systems: depicting the genomic
RT structure and the expression profiles of the gene families of uncoupling
RT protein and alternative oxidase in monocots and dicots.";
RL J. Exp. Bot. 57:849-864(2006).
CC -!- FUNCTION: PUMPS are mitochondrial transporter proteins that create
CC proton leaks across the inner mitochondrial membrane, thus uncoupling
CC oxidative phosphorylation. This leads to a decrease in the efficiency
CC of oxidative phosphorylation and an increase in heat production. May be
CC involved in protecting plant cells against oxidative stress damage (By
CC similarity). Recombinant PUMP6, reconstituted into liposomes,
CC transports a wide range of dicarboxylic acids including malate,
CC oxaloacetate and succinate as well as phosphate, sulfate and
CC thiosulfate. However, it is unknown if these transports are of any
CC biological significance in vivo. {ECO:0000250,
CC ECO:0000269|PubMed:18039180}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; AM236861; CAJ86453.1; -; mRNA.
DR EMBL; AL391712; CAC05473.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91400.1; -; Genomic_DNA.
DR EMBL; BT033087; ACF04810.1; -; mRNA.
DR RefSeq; NP_196509.1; NM_120984.2.
DR AlphaFoldDB; Q9FY68; -.
DR BioGRID; 16084; 3.
DR IntAct; Q9FY68; 1.
DR STRING; 3702.AT5G09470.1; -.
DR PaxDb; Q9FY68; -.
DR PRIDE; Q9FY68; -.
DR ProteomicsDB; 226126; -.
DR EnsemblPlants; AT5G09470.1; AT5G09470.1; AT5G09470.
DR GeneID; 830806; -.
DR Gramene; AT5G09470.1; AT5G09470.1; AT5G09470.
DR KEGG; ath:AT5G09470; -.
DR Araport; AT5G09470; -.
DR TAIR; locus:2184832; AT5G09470.
DR eggNOG; KOG0759; Eukaryota.
DR HOGENOM; CLU_015166_14_1_1; -.
DR InParanoid; Q9FY68; -.
DR OMA; DWYRKNF; -.
DR OrthoDB; 984118at2759; -.
DR PhylomeDB; Q9FY68; -.
DR PRO; PR:Q9FY68; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FY68; baseline and differential.
DR Genevisible; Q9FY68; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0005310; F:dicarboxylic acid transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015140; F:malate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015131; F:oxaloacetate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015141; F:succinate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015116; F:sulfate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015117; F:thiosulfate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0071423; P:malate transmembrane transport; IBA:GO_Central.
DR GO; GO:0006839; P:mitochondrial transport; IDA:TAIR.
DR GO; GO:0015729; P:oxaloacetate transport; IBA:GO_Central.
DR GO; GO:0035435; P:phosphate ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0071422; P:succinate transmembrane transport; IBA:GO_Central.
DR GO; GO:0008272; P:sulfate transport; IBA:GO_Central.
DR GO; GO:0015709; P:thiosulfate transport; IBA:GO_Central.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 2: Evidence at transcript level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Repeat; Stress response; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..337
FT /note="Mitochondrial uncoupling protein 6"
FT /id="PRO_0000420260"
FT TRANSMEM 6..26
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 4..136
FT /note="Solcar 1"
FT REPEAT 145..236
FT /note="Solcar 2"
FT REPEAT 246..331
FT /note="Solcar 3"
SQ SEQUENCE 337 AA; 36096 MW; B8FBA4C4A91D11CF CRC64;
MGFKPFLEGG IAAIIAGALT HPLDLIKVRM QLQGEHSFSL DQNPNPNLSL DHNLPVKPYR
PVFALDSLIG SISLLPLHIH APSSSTRSVM TPFAVGAHIV KTEGPAALFS GVSATILRQM
LYSATRMGIY DFLKRRWTDQ LTGNFPLVTK ITAGLIAGAV GSVVGNPADV AMVRMQADGS
LPLNRRRNYK SVVDAIDRIA RQEGVSSLWR GSWLTVNRAM IVTASQLATY DHVKEILVAG
GRGTPGGIGT HVAASFAAGI VAAVASNPID VVKTRMMNAD KEIYGGPLDC AVKMVAEEGP
MALYKGLVPT ATRQGPFTMI LFLTLEQVRG LLKDVKF
