ID   PUN1_CAPCH              Reviewed;         440 AA.
AC   A0A2G3D6U1; Q58VT0;
DT   10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT   31-JAN-2018, sequence version 1.
DT   03-AUG-2022, entry version 14.
DE   RecName: Full=Acyltransferase Pun1 {ECO:0000305};
DE            EC=2.3.2.35 {ECO:0000250|UniProtKB:D2Y3X2};
DE   AltName: Full=Acyltransferase 3 {ECO:0000303|PubMed:15918882};
DE   AltName: Full=Capsaicin synthase {ECO:0000305};
DE   AltName: Full=Protein PUNGENT 1 {ECO:0000303|PubMed:15918882};
GN   Name=PUN1 {ECO:0000303|PubMed:15918882};
GN   Synonyms=AT3 {ECO:0000303|PubMed:15918882};
GN   ORFNames=BC332_05016 {ECO:0000312|EMBL:PHU26684.1};
OS   Capsicum chinense (Scotch bonnet) (Bonnet pepper).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Capsiceae; Capsicum.
OX   NCBI_TaxID=80379;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RX   PubMed=15918882; DOI=10.1111/j.1365-313x.2005.02410.x;
RA   Stewart C., Kang B.C., Liu K., Mazourek M., Moore S.L., Yoo E.Y., Kim B.D.,
RA   Paran I., Jahn M.M.;
RT   "The Pun1 gene for pungency in pepper encodes a putative acyltransferase.";
RL   Plant J. 42:675-688(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. PI159236;
RX   PubMed=29089032; DOI=10.1186/s13059-017-1341-9;
RA   Kim S., Park J., Yeom S.I., Kim Y.M., Seo E., Kim K.T., Kim M.S., Lee J.M.,
RA   Cheong K., Shin H.S., Kim S.B., Han K., Lee J., Park M., Lee H.A.,
RA   Lee H.Y., Lee Y., Oh S., Lee J.H., Choi E., Choi E., Lee S.E., Jeon J.,
RA   Kim H., Choi G., Song H., Lee J., Lee S.C., Kwon J.K., Lee H.Y., Koo N.,
RA   Hong Y., Kim R.W., Kang W.H., Huh J.H., Kang B.C., Yang T.J., Lee Y.H.,
RA   Bennetzen J.L., Choi D.;
RT   "New reference genome sequences of hot pepper reveal the massive evolution
RT   of plant disease-resistance genes by retroduplication.";
RL   Genome Biol. 18:R210.1-R210.11(2017).
CC   -!- FUNCTION: Participates in the biosynthesis of capsaicinoids in pungent
CC       cultivars of Capsicum chinense. Capsaicinoids, the alkaloids
CC       responsible for the heat or pungency of chili pepper, are synthesized
CC       from phenylpropanoid intermediates in the placental tissue of chili
CC       peper fruit. Can transfer an acyl from 8-methylnon-6-enoyl-CoA to
CC       vanillylamine forming capsaicin and CoA.
CC       {ECO:0000250|UniProtKB:D2Y3X2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6E)-8-methylnon-6-enoyl-CoA + vanillylamine = capsaicin + CoA
CC         + H(+); Xref=Rhea:RHEA:63832, ChEBI:CHEBI:3374, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:149596, ChEBI:CHEBI:149597;
CC         EC=2.3.2.35; Evidence={ECO:0000250|UniProtKB:D2Y3X2};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63833;
CC         Evidence={ECO:0000250|UniProtKB:D2Y3X2};
CC   -!- TISSUE SPECIFICITY: Expressed in fruit 20 days post anthesis.
CC       {ECO:0000269|PubMed:15918882}.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; AY819027; AAV66309.1; -; Genomic_DNA.
DR   EMBL; MCIT02000002; PHU26684.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2G3D6U1; -.
DR   SMR; A0A2G3D6U1; -.
DR   STRING; 80379.A0A2G3D6U1; -.
DR   OrthoDB; 1130893at2759; -.
DR   Proteomes; UP000224522; Chromosome 2.
DR   GO; GO:0016755; F:aminoacyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0009821; P:alkaloid biosynthetic process; ISS:UniProtKB.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..440
FT                   /note="Acyltransferase Pun1"
FT                   /id="PRO_0000451976"
FT   ACT_SITE        169
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:D2Y3X2"
FT   ACT_SITE        384
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:D2Y3X2"
FT   CONFLICT        27
FT                   /note="T -> K (in Ref. 1; AAV66309)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        195
FT                   /note="T -> A (in Ref. 1; AAV66309)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        212
FT                   /note="T -> K (in Ref. 1; AAV66309)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        217
FT                   /note="T -> I (in Ref. 1; AAV66309)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        300
FT                   /note="T -> A (in Ref. 1; AAV66309)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   440 AA;  49274 MW;  9D727CA710A58420 CRC64;
     MAFALPSSLV SVCDKSFIKP SSLTPSTLRF HKLSFIDQSL SNMYIPCAFF YPKVQQRLED
     SKNSDELSHI AHLLQTSLSQ TLVSYYPYAG KLKDNATVDC NDMGAEFLSV RIKCSMSEIL
     DHPHASLAES IVLPKDLPWA NNCEGGNLLV VQVSKFDCGG IAISVCFSHK IGDGCSLLNF
     LNDWSSVTRD HTTTTLVPSP RFVGDSVFST KTYGSLTTPQ ILSDLNECVQ KRLIFPTDKL
     DALRAKVAEE SGVKNPTRAE VVSALLFKCA TKASSSMLPS KLVHFLNIRT MIKPRLPRNT
     IGNLSSIFSI EATNMQDMEL PTLVRNLRKE VEVAYKKDQV EQNELILEVV ESMREGKLPF
     ENMDGYENVY TCSNLCKYPY YTVDFGWGRP ERVCLGNGPS KNAFFLKDYK AGQGVEARVM
     LHKQQMSEFE RNEELLEFIA