PUN1_YEAST
ID PUN1_YEAST Reviewed; 263 AA.
AC Q06991; D6VZ51; Q6B322;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Protein PUN1;
DE AltName: Full=Plasma membrane protein up-regulated during nitrogen stress 1;
GN Name=PUN1; OrderedLocusNames=YLR414C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-260, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=14557538; DOI=10.1073/pnas.2135500100;
RA Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.;
RT "A subset of membrane-associated proteins is ubiquitinated in response to
RT mutations in the endoplasmic reticulum degradation machinery.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003).
RN [7]
RP INDUCTION.
RX PubMed=15116342; DOI=10.1002/yea.1109;
RA Boorsma A., de Nobel H., ter Riet B., Bargmann B., Brul S.,
RA Hellingwerf K.J., Klis F.M.;
RT "Characterization of the transcriptional response to cell wall stress in
RT Saccharomyces cerevisiae.";
RL Yeast 21:413-427(2004).
RN [8]
RP INDUCTION.
RX PubMed=16000714; DOI=10.1099/mic.0.27989-0;
RA Rodriguez-Pena J.M., Perez-Diaz R.M., Alvarez S., Bermejo C., Garcia R.,
RA Santiago C., Nombela C., Arroyo J.;
RT "The 'yeast cell wall chip' - a tool to analyse the regulation of cell wall
RT biogenesis in Saccharomyces cerevisiae.";
RL Microbiology 151:2241-2249(2005).
RN [9]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [10]
RP SUBCELLULAR LOCATION.
RX PubMed=18799621; DOI=10.1091/mbc.e08-05-0479;
RA Alvarez F.J., Douglas L.M., Rosebrock A., Konopka J.B.;
RT "The Sur7 protein regulates plasma membrane organization and prevents
RT intracellular cell wall growth in Candida albicans.";
RL Mol. Biol. Cell 19:5214-5225(2008).
RN [11]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS].
RX PubMed=19756047; DOI=10.1038/msb.2009.64;
RA Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.;
RT "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals
RT new roles for protein glycosylation in eukaryotes.";
RL Mol. Syst. Biol. 5:308-308(2009).
RN [12]
RP INDUCTION, FUNCTION, SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=20228058; DOI=10.1074/jbc.m110.114926;
RA Xu T., Shively C.A., Jin R., Eckwahl M.J., Dobry C.J., Song Q., Kumar A.;
RT "A profile of differentially abundant proteins at the yeast cell periphery
RT during pseudohyphal growth.";
RL J. Biol. Chem. 285:15476-15488(2010).
RN [13]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-260, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22106047; DOI=10.1002/pmic.201100166;
RA Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL Proteomics 12:236-240(2012).
CC -!- FUNCTION: Contributes to the wild-type cellular response to nitrogen
CC stress through signaling pathways that regulate the expression of genes
CC involved in amino acid biosynthesis. Required for wild-type filamentous
CC growth, cell growth, and cell-cell adhesion.
CC {ECO:0000269|PubMed:20228058}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:18799621, ECO:0000269|PubMed:20228058}; Multi-pass
CC membrane protein {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:18799621, ECO:0000269|PubMed:20228058}.
CC Note=Localizes to punctate patches in the plasma membrane that show
CC extensive colocalization with SUR7.
CC -!- INDUCTION: By the HOG1 MAPK, cell wall perturbations, and nitrogen
CC stress. Expression is controlled by the MSS11 pseudohyphal growth
CC transcription factor. {ECO:0000269|PubMed:15116342,
CC ECO:0000269|PubMed:16000714, ECO:0000269|PubMed:20228058}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:19756047}.
CC -!- MISCELLANEOUS: Present with 1660 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SUR7 family. {ECO:0000305}.
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DR EMBL; U20162; AAB67498.1; -; Genomic_DNA.
DR EMBL; AY692558; AAT92577.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09717.1; -; Genomic_DNA.
DR PIR; S59380; S59380.
DR RefSeq; NP_013518.3; NM_001182302.3.
DR AlphaFoldDB; Q06991; -.
DR BioGRID; 31673; 84.
DR STRING; 4932.YLR414C; -.
DR TCDB; 1.H.1.4.2; the claudin tight junction (claudin1) family.
DR iPTMnet; Q06991; -.
DR MaxQB; Q06991; -.
DR PaxDb; Q06991; -.
DR PRIDE; Q06991; -.
DR TopDownProteomics; Q06991; -.
DR EnsemblFungi; YLR414C_mRNA; YLR414C; YLR414C.
DR GeneID; 851132; -.
DR KEGG; sce:YLR414C; -.
DR SGD; S000004406; PUN1.
DR VEuPathDB; FungiDB:YLR414C; -.
DR eggNOG; ENOG502S4UA; Eukaryota.
DR HOGENOM; CLU_1147965_0_0_1; -.
DR InParanoid; Q06991; -.
DR OMA; ERVASPY; -.
DR BioCyc; YEAST:G3O-32476-MON; -.
DR PRO; PR:Q06991; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q06991; protein.
DR GO; GO:0051285; C:cell cortex of cell tip; IBA:GO_Central.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005933; C:cellular bud; HDA:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0031505; P:fungal-type cell wall organization; IMP:SGD.
DR InterPro; IPR017974; Claudin_CS.
DR InterPro; IPR009571; SUR7/Rim9-like_fungi.
DR Pfam; PF06687; SUR7; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Isopeptide bond; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Ubl conjugation.
FT CHAIN 1..263
FT /note="Protein PUN1"
FT /id="PRO_0000247353"
FT TOPO_DOM 1..6
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..143
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 165..172
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 194..223
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 245..263
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 100
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 209
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CROSSLNK 260
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:22106047"
FT CONFLICT 42
FT /note="E -> K (in Ref. 3; AAT92577)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 263 AA; 29251 MW; 10F31095CAD12827 CRC64;
MRNFFTLFFA AIFSLGALIL AIVACAGSTK NYSPINKIYC AELDLSQMKV STVLPSLSSA
TLSSLGLPSY INIGLWSYCT VDSSHNIQSC SSPHGIQNFN LSSLVYDNIN NNEALELMDS
VASVVLPEKL KSKMTYYNNL VKCMFITILI GIVLTFVNLV FNVLRWIIHI RPLTWFGAFF
SFFAFAALLV SIGSCLGTYS YIKYILKHNY SDYGISMSIG RNYQGLMWGA VVGALLNFIL
WCSVRSRPTV IYANAPIEEK PLI
