ID   PUO_KOCRO               Reviewed;         478 AA.
AC   P40974;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Putrescine oxidase;
DE            EC=1.4.3.10;
GN   Name=puo;
OS   Kocuria rosea (Deinococcus erythromyxa) (Micrococcus rubens).
OC   Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Kocuria.
OX   NCBI_TaxID=1275;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=ATCC 186 / DSM 20447 / JCM 11614 / NBRC 3768 / NCIMB 11696 / NCTC
RC   7523;
RX   PubMed=8473854; DOI=10.1099/00221287-139-3-425;
RA   Ishizuka H., Horinouchi S., Beppu T.;
RT   "Putrescine oxidase of Micrococcus rubens: primary structure and
RT   Escherichia coli.";
RL   J. Gen. Microbiol. 139:425-432(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O2 + putrescine = 4-aminobutanal + H2O2 + NH4(+);
CC         Xref=Rhea:RHEA:18273, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16240, ChEBI:CHEBI:28938, ChEBI:CHEBI:58264,
CC         ChEBI:CHEBI:326268; EC=1.4.3.10;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC   -!- SIMILARITY: Belongs to the flavin monoamine oxidase family.
CC       {ECO:0000305}.
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DR   EMBL; D12511; BAA02074.1; -; Genomic_DNA.
DR   PIR; A47693; A47693.
DR   AlphaFoldDB; P40974; -.
DR   SMR; P40974; -.
DR   BioCyc; MetaCyc:MON-23; -.
DR   BRENDA; 1.4.3.10; 3354.
DR   GO; GO:0050232; F:putrescine oxidase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002937; Amino_oxidase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR001613; Flavin_amine_oxidase.
DR   Pfam; PF01593; Amino_oxidase; 1.
DR   PRINTS; PR00757; AMINEOXDASEF.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; FAD; Flavoprotein; Oxidoreductase.
FT   CHAIN           1..478
FT                   /note="Putrescine oxidase"
FT                   /id="PRO_0000099866"
FT   BINDING         15..70
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   478 AA;  51914 MW;  9C4567051B010582 CRC64;
     MTDQRTLGSE TAIERDVVVV GAGPAGLMAA RTLVAAGRTV AVLEARDRVG GRTWSKTVDG
     AFLEIGGQWI SPDQTELLAL VDELGLETYQ RYREGESVYL APDGTRHTYT GSMFPAGEST
     IVEMEKLVAL LDGLVAEIGA TEPWAHPAAR ELDTISFHHW LRQHSDDEAA CSNIGIFVAG
     GMLTKPAHAF SVLQAVLMAA SAGSFSNLVD EDFILDRRVV GGMQSVSETM AAELGEDVVF
     LDTPVRTIRW AGDGGTYAEH VPGTPVTVWS DRLTVRAKDV VVAVPPNLYS RISFEPPLPR
     LQHQMHQHQS LGLVIKVHAV YETPFWRDKG LSGTGFGAHE LSQEVYDNTN HGDPRGTLVG
     FVSDERADEL FGLPAEERRR LILESLSHYL GEEALHPVVY YESDFGSEEW TRGAYAASYD
     LGGLHRYGAH QRTPVGPIRW ACSDLAAEGY QHVDGALRQG RLAAAEVLGA GSLTGAER