PUP14_ARATH
ID PUP14_ARATH Reviewed; 393 AA.
AC Q9FXH5;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Purine permease 14 {ECO:0000303|PubMed:10662864};
DE Short=AtPUP14 {ECO:0000303|PubMed:10662864};
GN Name=PUP14 {ECO:0000303|PubMed:10662864};
GN OrderedLocusNames=At1g19770 {ECO:0000312|Araport:AT1G19770};
GN ORFNames=F14P1.13, F6F9.18 {ECO:0000312|EMBL:AAG12553.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=10662864; DOI=10.2307/3870929;
RA Gillissen B., Buerkle L., Andre B., Kuehn C., Rentsch D., Brandl B.,
RA Frommer W.B.;
RT "A new family of high-affinity transporters for adenine, cytosine, and
RT purine derivatives in Arabidopsis.";
RL Plant Cell 12:291-300(2000).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=27701112; DOI=10.1126/science.aaf7254;
RA Zuercher E., Liu J., di Donato M., Geisler M., Mueller B.;
RT "Plant development regulated by cytokinin sinks.";
RL Science 353:1027-1030(2016).
CC -!- FUNCTION: Purine permease implicated in ATP-dependent cytokinin
CC translocation that controls the spatiotemporal landscape of cytokinin
CC signaling. Depletes ligands from the apoplast, which leads to a
CC suppression of the cytokinin response. {ECO:0000269|PubMed:27701112}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:27701112};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, leaves, embryos, ovules,
CC seeds and the root and shoot meristems. In heart-stage embryos,
CC detected in cells that failed to respond to cytokinins, including the
CC prospective cotyledons. {ECO:0000269|PubMed:27701112}.
CC -!- SIMILARITY: Belongs to the purine permeases (TC 2.A.7.14) family.
CC {ECO:0000305}.
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DR EMBL; AC007797; AAG12553.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29895.1; -; Genomic_DNA.
DR EMBL; BX814406; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; G86330; G86330.
DR RefSeq; NP_173407.1; NM_101833.4.
DR AlphaFoldDB; Q9FXH5; -.
DR BioGRID; 23804; 11.
DR IntAct; Q9FXH5; 9.
DR STRING; 3702.AT1G19770.1; -.
DR iPTMnet; Q9FXH5; -.
DR PaxDb; Q9FXH5; -.
DR PRIDE; Q9FXH5; -.
DR ProteomicsDB; 226364; -.
DR EnsemblPlants; AT1G19770.1; AT1G19770.1; AT1G19770.
DR GeneID; 838565; -.
DR Gramene; AT1G19770.1; AT1G19770.1; AT1G19770.
DR KEGG; ath:AT1G19770; -.
DR Araport; AT1G19770; -.
DR TAIR; locus:2013124; AT1G19770.
DR eggNOG; ENOG502QVMQ; Eukaryota.
DR HOGENOM; CLU_043459_2_1_1; -.
DR InParanoid; Q9FXH5; -.
DR OMA; WPTITIS; -.
DR OrthoDB; 822261at2759; -.
DR PhylomeDB; Q9FXH5; -.
DR PRO; PR:Q9FXH5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FXH5; baseline and differential.
DR Genevisible; Q9FXH5; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; TAS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005345; F:purine nucleobase transmembrane transporter activity; TAS:TAIR.
DR GO; GO:0015211; F:purine nucleoside transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0010184; P:cytokinin transport; IDA:TAIR.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0080037; P:negative regulation of cytokinin-activated signaling pathway; IMP:TAIR.
DR GO; GO:0006863; P:purine nucleobase transport; TAS:TAIR.
DR InterPro; IPR030182; PUP_plant.
DR PANTHER; PTHR31376; PTHR31376; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell membrane; Cytokinin signaling pathway; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..393
FT /note="Purine permease 14"
FT /id="PRO_0000317401"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 363..383
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 393 AA; 44063 MW; C430322437E0BC24 CRC64;
MAQNQQPIFQ TKPPEQFVQI PINIERDSST TRMNQTGNTI RKPNHWPTIT ISIIFVIIGQ
SIAKLLENFY YDKTNRSEYN ENRQNDGVWT QSLLQTVGFP LLLLPFLIFI TKNKRNHHQQ
PPITSDSIHL KSLAVIYICI GIIMSVQGRL AAMGKLEIPF GVFTLIYTAQ LFFTPIFAAF
INKIKFNRWV VISVILAIIT GALTLSSSFG GEPDEAEENY ARGSWAALFA GICFALLLCN
IQNVFDSYIF KRTESTNQKP SFASVFEVII FSSLVATIIS VVGLLIAGEQ HDLKREMNGF
SKGKGSYVMA MVGQAVSWQV YWVGIVGLVY SVSSVLSNVI SVITWPIVSV LVVIFFNFMD
DEFDAFKGVA LVTAVLSAAA YFFRLHKDNR MAY
