PUP1_CANGA
ID PUP1_CANGA Reviewed; 211 AA.
AC Q6FIN8;
DT 12-SEP-2018, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=PDR1 up-regulated protein 1 {ECO:0000303|PubMed:21408004};
GN Name=PUP1 {ECO:0000303|PubMed:21408004}; OrderedLocusNames=CAGL0M12947g;
OS Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS Y-65) (Yeast) (Torulopsis glabrata).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC Nakaseomyces/Candida clade.
OX NCBI_TaxID=284593;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
RN [2]
RP FUNCTION, INDUCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=21408004; DOI=10.1371/journal.pone.0017589;
RA Ferrari S., Sanguinetti M., Torelli R., Posteraro B., Sanglard D.;
RT "Contribution of CgPDR1-regulated genes in enhanced virulence of azole-
RT resistant Candida glabrata.";
RL PLoS ONE 6:E17589-E17589(2011).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23460523; DOI=10.1128/iai.00074-13;
RA Vale-Silva L., Ischer F., Leibundgut-Landmann S., Sanglard D.;
RT "Gain-of-function mutations in PDR1, a regulator of antifungal drug
RT resistance in Candida glabrata, control adherence to host cells.";
RL Infect. Immun. 81:1709-1720(2013).
RN [4]
RP INDUCTION.
RX PubMed=25199772; DOI=10.1128/aac.03921-14;
RA Paul S., Bair T.B., Moye-Rowley W.S.;
RT "Identification of genomic binding sites for Candida glabrata Pdr1
RT transcription factor in wild-type and rho0 cells.";
RL Antimicrob. Agents Chemother. 58:6904-6912(2014).
RN [5]
RP INDUCTION.
RX PubMed=29648590; DOI=10.1093/femsyr/foy038;
RA Tian Y., Gao N., Ni Q., Mao Y., Dong D., Huang X., Jiang C., Li Z.,
RA Zhang L., Wang X., Peng Y., Chen C.;
RT "Sequence modification of the master regulator Pdr1 interferes with its
RT transcriptional autoregulation and confers altered azole resistance in
RT Candida glabrata.";
RL FEMS Yeast Res. 18:0-0(2018).
CC -!- FUNCTION: Mitochondrial protein that contributes to the enhanced
CC virulence of C.glabrata strains that acquired azole resistance.
CC {ECO:0000269|PubMed:21408004, ECO:0000269|PubMed:23460523}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane
CC {ECO:0000269|PubMed:21408004}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- INDUCTION: Expression is inducible by fluconazole (PubMed:21408004).
CC Expression is positively regulated by the master transcriptional
CC regulator PDR1 which probably binds the PDRE motifs TCCACGGA (positions
CC -770 to -763) and TCCGTGGA (positions -740 to -733) of its promoter
CC (PubMed:21408004, PubMed:25199772, PubMed:29648590).
CC {ECO:0000269|PubMed:21408004, ECO:0000269|PubMed:25199772,
CC ECO:0000269|PubMed:29648590}.
CC -!- DISRUPTION PHENOTYPE: Leads to an increase in phagocytosis but does not
CC influence binding to host macrophages in infected mice, when the
CC pleiotropic ABC efflux transporter of multiple drugs CDR1 is also
CC deleted. {ECO:0000269|PubMed:23460523}.
CC -!- SIMILARITY: Belongs to the PUP1 family.
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DR EMBL; CR380959; CAG62886.1; -; Genomic_DNA.
DR RefSeq; XP_449906.1; XM_449906.1.
DR AlphaFoldDB; Q6FIN8; -.
DR STRING; 5478.XP_449906.1; -.
DR EnsemblFungi; CAG62886; CAG62886; CAGL0M12947g.
DR GeneID; 2891196; -.
DR KEGG; cgr:CAGL0M12947g; -.
DR CGD; CAL0136385; PUP1.
DR VEuPathDB; FungiDB:CAGL0M12947g; -.
DR eggNOG; ENOG502S01E; Eukaryota.
DR HOGENOM; CLU_1304705_0_0_1; -.
DR InParanoid; Q6FIN8; -.
DR Proteomes; UP000002428; Chromosome M.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IDA:CGD.
DR InterPro; IPR012470; Pup1-like.
DR Pfam; PF07954; DUF1689; 1.
PE 2: Evidence at transcript level;
KW Membrane; Mitochondrion; Reference proteome; Transmembrane;
KW Transmembrane helix; Virulence.
FT CHAIN 1..211
FT /note="PDR1 up-regulated protein 1"
FT /id="PRO_0000445083"
FT TRANSMEM 45..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..99
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 211 AA; 24379 MW; 9540F75311DCA750 CRC64;
MSDSREIKKP WWFKKAKEWA DEFYTRDQKL EDSDRRDLSK KYATISKASV VGAATGLSAG
LGGPYAYRYY TTGALKGVKL PRTILLGVVS MVIMRNVAAK IAWDKELKKL DPSGELKQNY
KSAHKTVDDV SLSDNSTSVQ KKYGMMKFLK YRTAPRWAMY FEGTYQHPDR KFPNPDQMVQ
DLKGSRRAFP PFFSKADKFW HQIDRKDNDH T
