PUP1_YEAST
ID PUP1_YEAST Reviewed; 320 AA.
AC P40508; D6VVK7;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=PUP1 protein homolog;
GN OrderedLocusNames=YIL077C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=16823961; DOI=10.1021/pr050477f;
RA Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.;
RT "Toward the complete yeast mitochondrial proteome: multidimensional
RT separation techniques for mitochondrial proteomics.";
RL J. Proteome Res. 5:1543-1554(2006).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-230, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane
CC {ECO:0000269|PubMed:16823961}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- MISCELLANEOUS: Present with 922 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the PUP1 family.
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DR EMBL; Z37997; CAA86093.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08473.1; -; Genomic_DNA.
DR PIR; S48367; S48367.
DR RefSeq; NP_012188.3; NM_001179427.3.
DR AlphaFoldDB; P40508; -.
DR BioGRID; 34915; 103.
DR DIP; DIP-5434N; -.
DR IntAct; P40508; 1.
DR STRING; 4932.YIL077C; -.
DR iPTMnet; P40508; -.
DR MaxQB; P40508; -.
DR PaxDb; P40508; -.
DR PRIDE; P40508; -.
DR EnsemblFungi; YIL077C_mRNA; YIL077C; YIL077C.
DR GeneID; 854733; -.
DR KEGG; sce:YIL077C; -.
DR SGD; S000001339; YIL077C.
DR VEuPathDB; FungiDB:YIL077C; -.
DR eggNOG; ENOG502S01E; Eukaryota.
DR HOGENOM; CLU_072880_0_0_1; -.
DR InParanoid; P40508; -.
DR OMA; FYEKDSA; -.
DR BioCyc; YEAST:G3O-31342-MON; -.
DR PRO; PR:P40508; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P40508; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR InterPro; IPR012470; Pup1-like.
DR Pfam; PF07954; DUF1689; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..320
FT /note="PUP1 protein homolog"
FT /id="PRO_0000202980"
FT TRANSMEM 66..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 205..320
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 239..273
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 290..320
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 230
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 320 AA; 36946 MW; CD2BDDA0408A9147 CRC64;
MLGKEEEQQY GQNGKGMENE LPFMKRPWFK KAYENAIEFH EKDELLDARD RLELSKAYRS
IAKAEMWGGW LGFSAVFLTP FAYRYYKTKA IKGVKVPRNF VLGVMALFFA TNFAGRSMYT
RQLNERDPTG VLKDNYSNKY GDNDFGAFQH DQTKEIPRNQ RQYNMMRLLD SGSPSRWSMY
FYITYQNPER RLPDPKVKLQ QMKKGGVFNG SPFMNQRDPI GLYRNKGRKS PDPIEGEQND
SPVLSSWEKI RNGDNSSSSS WENIRNTSRD QSQESDASVD HESDIFISGF SDDGNATDNS
SSDDKYQRLL QSGRYGGNRS
