PUP21_ARATH
ID PUP21_ARATH Reviewed; 390 AA.
AC O49726;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 22-FEB-2012, sequence version 2.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Purine permease 21 {ECO:0000303|PubMed:27701112};
DE Short=AtPUP21 {ECO:0000303|PubMed:27701112};
GN Name=PUP21 {ECO:0000303|PubMed:27701112};
GN OrderedLocusNames=At4g18220 {ECO:0000312|Araport:AT4G18220};
GN ORFNames=T9A21.70 {ECO:0000312|EMBL:CAA16794.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP TISSUE SPECIFICITY, NOMENCLATURE, AND GENE FAMILY.
RX PubMed=27701112; DOI=10.1126/science.aaf7254;
RA Zuercher E., Liu J., di Donato M., Geisler M., Mueller B.;
RT "Plant development regulated by cytokinin sinks.";
RL Science 353:1027-1030(2016).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in mesophyll cells.
CC {ECO:0000269|PubMed:27701112}.
CC -!- SIMILARITY: Belongs to the purine permeases (TC 2.A.7.14) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA16794.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78824.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL021713; CAA16794.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161548; CAB78824.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE84013.2; -; Genomic_DNA.
DR PIR; T04924; T04924.
DR RefSeq; NP_001319981.1; NM_001341267.1.
DR AlphaFoldDB; O49726; -.
DR SMR; O49726; -.
DR BioGRID; 12841; 21.
DR IntAct; O49726; 1.
DR STRING; 3702.AT4G18220.1; -.
DR PaxDb; O49726; -.
DR PRIDE; O49726; -.
DR ProteomicsDB; 226116; -.
DR EnsemblPlants; AT4G18220.1; AT4G18220.1; AT4G18220.
DR GeneID; 827548; -.
DR Gramene; AT4G18220.1; AT4G18220.1; AT4G18220.
DR KEGG; ath:AT4G18220; -.
DR Araport; AT4G18220; -.
DR eggNOG; ENOG502QVMQ; Eukaryota.
DR InParanoid; O49726; -.
DR OMA; WIQMIIY; -.
DR OrthoDB; 789193at2759; -.
DR PRO; PR:O49726; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O49726; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005345; F:purine nucleobase transmembrane transporter activity; IEA:UniProt.
DR GO; GO:0015211; F:purine nucleoside transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR030182; PUP_plant.
DR PANTHER; PTHR31376; PTHR31376; 1.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..390
FT /note="Purine permease 21"
FT /id="PRO_0000317396"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 287..307
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 312..332
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 12..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 367..390
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 372..390
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 390 AA; 43087 MW; AADBBA3CE4E85E1C CRC64;
MKGDQEVQVI VQQGKEPIPT DQDERSSVSG SQTKLSHSNT YKRWLRVAIY TFFVISGQSV
ATILGRLYYE NGGNSKWLAT VVQLVGFPIL LPYHLLSVKT HTTTQRDGKL TSLRNRALVY
IVLGLLVGAA CYLYSIGLLY LPVSTLSLIC ASQLAFTAFF SYLLNSQKLT PIILNSLFLL
TISSTLLAFN NEESDSKKVT KGEYVKGFVC TVGASAGFGL LLSLQQLAFR KVLKKQTFSE
VINMIIYMSL VASCVSVVGL FASSEWKTLS SEMENYKLGK VSYVMNLVWT AVTWQVFSIG
CTGLIFELSS LFSNAISALG LPVVPILAVI IFHDKMNGLK VISMILAIWG FVSYVYQQYL
DETNLKKSNE IPTTESPDRP EAEGSSEQSK
