PUP2_ARATH
ID PUP2_ARATH Reviewed; 358 AA.
AC Q94GB1; P93010; Q3EBN9;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Purine permease 2;
DE Short=AtPUP2;
GN Name=PUP2; OrderedLocusNames=At2g33750; ORFNames=T1B8.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RX PubMed=10662864; DOI=10.2307/3870929;
RA Gillissen B., Buerkle L., Andre B., Kuehn C., Rentsch D., Brandl B.,
RA Frommer W.B.;
RT "A new family of high-affinity transporters for adenine, cytosine, and
RT purine derivatives in Arabidopsis.";
RL Plant Cell 12:291-300(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ACTIVITY REGULATION, TISSUE
RP SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12662305; DOI=10.1046/j.1365-313x.2003.01700.x;
RA Buerkle L., Cedzich A., Doepke C., Stransky H., Okumoto S., Gillissen B.,
RA Kuehn C., Frommer W.B.;
RT "Transport of cytokinins mediated by purine transporters of the PUP family
RT expressed in phloem, hydathodes, and pollen of Arabidopsis.";
RL Plant J. 34:13-26(2003).
CC -!- FUNCTION: Mediates adenine transport. May be involved in the uptake of
CC cytokinin analogs. {ECO:0000269|PubMed:12662305}.
CC -!- ACTIVITY REGULATION: Competitive inhibition of adenine transport by
CC isopentenyladenine, kinetin, benzylaminopurine, trans- and cis-zeatin
CC and trans-zeatin riboside. {ECO:0000269|PubMed:12662305}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=22.6 uM for adenine (at pH 3.5) {ECO:0000269|PubMed:12662305};
CC pH dependence:
CC Optimum pH is 3.0. {ECO:0000269|PubMed:12662305};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q94GB1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q94GB1-2; Sequence=VSP_030944;
CC -!- TISSUE SPECIFICITY: Expressed in the vascular system of leaves.
CC Restricted to the phloem. Expressed in flowers and roots and not
CC detected in stems. {ECO:0000269|PubMed:12662305}.
CC -!- SIMILARITY: Belongs to the purine permeases (TC 2.A.7.14) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC69140.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF078532; AAK61813.1; -; mRNA.
DR EMBL; U78721; AAC69140.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002685; AEC08879.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08880.1; -; Genomic_DNA.
DR PIR; B84749; B84749.
DR RefSeq; NP_180931.2; NM_128934.3. [Q94GB1-1]
DR RefSeq; NP_973592.1; NM_201863.2. [Q94GB1-2]
DR AlphaFoldDB; Q94GB1; -.
DR STRING; 3702.AT2G33750.1; -.
DR TCDB; 2.A.7.14.4; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; Q94GB1; -.
DR PRIDE; Q94GB1; -.
DR ProteomicsDB; 226119; -. [Q94GB1-1]
DR EnsemblPlants; AT2G33750.1; AT2G33750.1; AT2G33750. [Q94GB1-1]
DR EnsemblPlants; AT2G33750.2; AT2G33750.2; AT2G33750. [Q94GB1-2]
DR GeneID; 817941; -.
DR Gramene; AT2G33750.1; AT2G33750.1; AT2G33750. [Q94GB1-1]
DR Gramene; AT2G33750.2; AT2G33750.2; AT2G33750. [Q94GB1-2]
DR KEGG; ath:AT2G33750; -.
DR Araport; AT2G33750; -.
DR TAIR; locus:2057666; AT2G33750.
DR eggNOG; ENOG502QTN9; Eukaryota.
DR InParanoid; Q94GB1; -.
DR OMA; NESYREY; -.
DR OrthoDB; 1112849at2759; -.
DR PhylomeDB; Q94GB1; -.
DR BioCyc; MetaCyc:MON-14778; -.
DR SABIO-RK; Q94GB1; -.
DR PRO; PR:Q94GB1; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q94GB1; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; TAS:TAIR.
DR GO; GO:0005345; F:purine nucleobase transmembrane transporter activity; TAS:TAIR.
DR GO; GO:0015211; F:purine nucleoside transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006863; P:purine nucleobase transport; TAS:TAIR.
DR InterPro; IPR030182; PUP_plant.
DR PANTHER; PTHR31376; PTHR31376; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..358
FT /note="Purine permease 2"
FT /id="PRO_0000317389"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 37..57
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..282
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 312..332
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 46..154
FT /note="EamA"
FT VAR_SEQ 233..243
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_030944"
SQ SEQUENCE 358 AA; 39461 MW; 69709A0EA6ECD6A8 CRC64;
MKMKTVLVII NCIFLAIGNC GGPLMMRLYF QNGGERIWFP SFLQTVGCPL IFFPLLLSFL
RRRRCLEEQE TTPFFLMKPP LFIAAIVVGL LVGFDNYLYS YGLAYIPVST ASLIISAQLG
FTALFAFFMV KQKFTPFTIN AIVLLTGGAV VLALNSDSDK LANETHKEYV VGFIMTLGAA
LLYGFILPLV ELSYKKSGQR ITYTLALEFQ MVLCFAATCV CLVGMLAAGD FKVKHALFIF
KNRVIAGEAR DFKLGESLYY VVIVFTAIIW QAFFVGAIGL IFCASSLVSG IMVSALLPVT
VILAVICFQE KFQAGKGVAL ALSLWGSVSY FYGQVKSEEK TKAQDTQLSQ LPVTDYVA
