PUP3_ARATH
ID PUP3_ARATH Reviewed; 351 AA.
AC Q9FZ95;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Purine permease 3;
DE Short=AtPUP3;
GN Name=PUP3; OrderedLocusNames=At1g28220; ORFNames=F3H9.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=10662864; DOI=10.2307/3870929;
RA Gillissen B., Buerkle L., Andre B., Kuehn C., Rentsch D., Brandl B.,
RA Frommer W.B.;
RT "A new family of high-affinity transporters for adenine, cytosine, and
RT purine derivatives in Arabidopsis.";
RL Plant Cell 12:291-300(2000).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12662305; DOI=10.1046/j.1365-313x.2003.01700.x;
RA Buerkle L., Cedzich A., Doepke C., Stransky H., Okumoto S., Gillissen B.,
RA Kuehn C., Frommer W.B.;
RT "Transport of cytokinins mediated by purine transporters of the PUP family
RT expressed in phloem, hydathodes, and pollen of Arabidopsis.";
RL Plant J. 34:13-26(2003).
CC -!- FUNCTION: May be involved in transport of purine derivatives during
CC pollen germination and tube elongation. {ECO:0000269|PubMed:12662305}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Restricted to pollen.
CC {ECO:0000269|PubMed:12662305}.
CC -!- SIMILARITY: Belongs to the purine permeases (TC 2.A.7.14) family.
CC {ECO:0000305}.
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DR EMBL; AC021044; AAF98433.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30933.1; -; Genomic_DNA.
DR PIR; C86408; C86408.
DR RefSeq; NP_174143.1; NM_102587.2.
DR AlphaFoldDB; Q9FZ95; -.
DR SMR; Q9FZ95; -.
DR BioGRID; 24951; 13.
DR IntAct; Q9FZ95; 12.
DR STRING; 3702.AT1G28220.1; -.
DR PaxDb; Q9FZ95; -.
DR PRIDE; Q9FZ95; -.
DR EnsemblPlants; AT1G28220.1; AT1G28220.1; AT1G28220.
DR GeneID; 839716; -.
DR Gramene; AT1G28220.1; AT1G28220.1; AT1G28220.
DR KEGG; ath:AT1G28220; -.
DR Araport; AT1G28220; -.
DR TAIR; locus:2032159; AT1G28220.
DR eggNOG; ENOG502QTN9; Eukaryota.
DR HOGENOM; CLU_043459_1_1_1; -.
DR InParanoid; Q9FZ95; -.
DR OMA; IACAYMY; -.
DR OrthoDB; 1112849at2759; -.
DR PhylomeDB; Q9FZ95; -.
DR PRO; PR:Q9FZ95; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FZ95; baseline and differential.
DR Genevisible; Q9FZ95; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; TAS:TAIR.
DR GO; GO:0005345; F:purine nucleobase transmembrane transporter activity; TAS:TAIR.
DR GO; GO:0015211; F:purine nucleoside transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006863; P:purine nucleobase transport; TAS:TAIR.
DR InterPro; IPR030182; PUP_plant.
DR PANTHER; PTHR31376; PTHR31376; 1.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..351
FT /note="Purine permease 3"
FT /id="PRO_0000317390"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 45..152
FT /note="EamA"
FT REGION 329..351
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 337..351
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 351 AA; 38871 MW; 992F1704CB76ED70 CRC64;
MVKALVIINC IILAIGNCGG PLIMRLYFNN GGKRIWFSTF LETAGFPVIF IPLLFSYITR
RRSNNVGDST SFFLIKPRLL IAAVIVGILS GFDNYLYAYG IAYLPVSTAA LIIASQLAFI
AIFSFFMVKH KFTPFTINAV VLLTVGAAVL GMHTETDKPV HETHKQYITG FLITVAAAVM
YAFILPLVEL AYQKAKQTMS YTLVLEFQLI LCLLASIVSV IGMFIAGDFK ALPKEAREFK
LGEALFYVVA VFSAIIWQGF FLGAIGLIFS TSSLVSGIMI SVLLPITEVL AVIFYHEKFQ
AEKGLSLALS LWGFVSYFYG EIKSGEDKRR IQQEESQETE QSSLSRPISE C
