PUP8_ARATH
ID PUP8_ARATH Reviewed; 394 AA.
AC Q0WRB9; O49724;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=Probable purine permease 8;
DE Short=AtPUP8;
GN Name=PUP8; OrderedLocusNames=At4g18195; ORFNames=T9A21.50;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=10662864; DOI=10.2307/3870929;
RA Gillissen B., Buerkle L., Andre B., Kuehn C., Rentsch D., Brandl B.,
RA Frommer W.B.;
RT "A new family of high-affinity transporters for adenine, cytosine, and
RT purine derivatives in Arabidopsis.";
RL Plant Cell 12:291-300(2000).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the purine permeases (TC 2.A.7.14) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA16792.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g18200 has been split into 3 genes: At4g18195, At4g18197 and At4g18205.; Evidence={ECO:0000305};
CC Sequence=CAB78822.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g18200 has been split into 3 genes: At4g18195, At4g18197 and At4g18205.; Evidence={ECO:0000305};
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DR EMBL; AL021713; CAA16792.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161548; CAB78822.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE84009.1; -; Genomic_DNA.
DR EMBL; AK228393; BAF00330.1; -; mRNA.
DR RefSeq; NP_001031662.1; NM_001036585.3.
DR AlphaFoldDB; Q0WRB9; -.
DR BioGRID; 530663; 7.
DR IntAct; Q0WRB9; 7.
DR STRING; 3702.AT4G18195.1; -.
DR PaxDb; Q0WRB9; -.
DR PRIDE; Q0WRB9; -.
DR EnsemblPlants; AT4G18195.1; AT4G18195.1; AT4G18195.
DR GeneID; 3770302; -.
DR Gramene; AT4G18195.1; AT4G18195.1; AT4G18195.
DR KEGG; ath:AT4G18195; -.
DR Araport; AT4G18195; -.
DR TAIR; locus:1009023376; AT4G18195.
DR eggNOG; ENOG502QVMQ; Eukaryota.
DR HOGENOM; CLU_043459_2_1_1; -.
DR InParanoid; Q0WRB9; -.
DR OMA; MNASKIF; -.
DR OrthoDB; 789193at2759; -.
DR PhylomeDB; Q0WRB9; -.
DR PRO; PR:Q0WRB9; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q0WRB9; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; TAS:TAIR.
DR GO; GO:0005345; F:purine nucleobase transmembrane transporter activity; TAS:TAIR.
DR GO; GO:0015211; F:purine nucleoside transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006863; P:purine nucleobase transport; TAS:TAIR.
DR InterPro; IPR030182; PUP_plant.
DR PANTHER; PTHR31376; PTHR31376; 1.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..394
FT /note="Probable purine permease 8"
FT /id="PRO_0000317395"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 247..267
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 344..364
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 373..394
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 394 AA; 43537 MW; ABA609B042EE8F96 CRC64;
MEITQVIYVN DHNNIEANLT GQEEMNTTME IESSSVPQSK NYKKWLRISI YVFFVLACQA
LSTILGRVYY ENGGKSTWMG TLVQLIGFPV LFLFRFFSQT KNPKPTEADF RKFSSFTILG
SVYIVTGLLV SANSYMSSVG LLYLPVSTFS LILASQLAFT AFFSYFLNSQ KFTPFIVNSL
FLLTISSALL VVNTDSENTA KVSRVKYVIG IICTIGASAG IGLLLSLVQL ILRKVLKKQT
FSTVTDLVAY QSLVASCVVL IGLFASGEWK TLTSEMENYK LGKVPYVMTL ASIAISWQVY
TIGVVGLIFE SSSVFSNSIT AVGLPIVPVV AVIVFHDKMN ASKIFSIILA IWGFISFVYQ
HYLDEKKLKT SHTSPVGDPH LLPAEEGHTN IHSV
