ID   AADK_CAMJU              Reviewed;         288 AA.
AC   P0DTV9;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   17-JUN-2020, sequence version 1.
DT   25-MAY-2022, entry version 9.
DE   RecName: Full=Aminoglycoside 6-adenylyltransferase {ECO:0000303|PubMed:15917546};
DE            EC=2.7.7.- {ECO:0000305|PubMed:15917546};
DE   AltName: Full=ORF2 {ECO:0000303|PubMed:15917546};
DE   AltName: Full=Streptomycin 6-adenylyltransferase {ECO:0000303|PubMed:15917546};
GN   Name=aadK {ECO:0000305};
GN   Synonyms=aadE {ECO:0000303|PubMed:15917546}, ant(6) {ECO:0000305};
GN   ORFNames=C9J79_09595;
OS   Campylobacter jejuni.
OG   Plasmid pCG8245.
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Campylobacteraceae; Campylobacter.
OX   NCBI_TaxID=197;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC   STRAIN=CG8245 / Serotype Lior 19; PLASMID=pCG8245;
RX   PubMed=15917546; DOI=10.1128/aac.49.6.2454-2459.2005;
RA   Nirdnoy W., Mason C.J., Guerry P.;
RT   "Mosaic structure of a multiple-drug-resistant, conjugative plasmid from
RT   Campylobacter jejuni.";
RL   Antimicrob. Agents Chemother. 49:2454-2459(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=C179b;
RX   PubMed=25331057; DOI=10.1093/jac/dku418;
RA   Deng F., Wang Y., Zhang Y., Shen Z.;
RT   "Characterization of the genetic environment of the ribosomal RNA methylase
RT   gene erm(B) in Campylobacter jejuni.";
RL   J. Antimicrob. Chemother. 70:613-615(2015).
CC   -!- FUNCTION: Mediates bacterial resistance to streptomycin, is probably a
CC       streptomycin 6-adenylyltransferase. {ECO:0000269|PubMed:15917546,
CC       ECO:0000305|PubMed:25331057}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + streptomycin = 6-O-adenylylstreptomycin + diphosphate;
CC         Xref=Rhea:RHEA:63236, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58007, ChEBI:CHEBI:146262;
CC         Evidence={ECO:0000305|PubMed:15917546};
CC   -!- DISRUPTION PHENOTYPE: Loss of streptomycin resistance.
CC       {ECO:0000269|PubMed:15917546}.
CC   -!- MISCELLANEOUS: In strain C179b a 10242 bp segment of DNA has been
CC       inserted into the chromosome between cj0168c and sodB. This piece of
CC       DNA forms a multidrug resistance genomic island and is transferable
CC       among C.jejuni strains. {ECO:0000269|PubMed:25331057}.
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DR   EMBL; AY701528; AAW34139.1; -; Genomic_DNA.
DR   EMBL; KF864551; AIJ27543.1; -; Genomic_DNA.
DR   RefSeq; WP_001255868.1; NZ_VOEN01000013.1.
DR   AlphaFoldDB; P0DTV9; -.
DR   SMR; P0DTV9; -.
DR   GeneID; 60291862; -.
DR   GeneID; 66773966; -.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IMP:UniProtKB.
DR   Gene3D; 3.30.460.10; -; 1.
DR   InterPro; IPR007530; Aminoglycoside_adenylylTfrase.
DR   InterPro; IPR043519; NT_sf.
DR   Pfam; PF04439; Adenyl_transf; 1.
DR   PIRSF; PIRSF000812; AAD; 1.
DR   SUPFAM; SSF81301; SSF81301; 1.
PE   4: Predicted;
KW   Antibiotic resistance; Nucleotidyltransferase; Plasmid; Transferase.
FT   CHAIN           1..288
FT                   /note="Aminoglycoside 6-adenylyltransferase"
FT                   /id="PRO_0000450272"
SQ   SEQUENCE   288 AA;  34552 MW;  8C52BA3E9AD0C5C2 CRC64;
     MRSEKEVYDI VLNFAKTDKR IRMVTLEGSR TNTNIPPDDF QDFDITFFVT DMDSFTSDDK
     WLDIFGERLI LQKPEDMELF PAVEKGFSYL MLFTDDVKID LTLLPLELID EYFTWDKLVK
     LLLDKDNRIV KPPIPTDIDY HLQKPTQRMF DDCCNEFWNT TTYVVKGLCR KEILFAIDHM
     NDIVRKELLR MISWLIGIKQ GFHFSLGKNY KFMKQYVPEE LWERLMSTYN MDSYPHMWES
     FEQCMALFRE VSSEVACQLD YQYPLYDEKI SNYVIRQKKK YGIEDDNK