ID   PUR2_HELPJ              Reviewed;         424 AA.
AC   Q9ZK07;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Phosphoribosylamine--glycine ligase;
DE            EC=6.3.4.13;
DE   AltName: Full=GARS;
DE   AltName: Full=Glycinamide ribonucleotide synthetase;
DE   AltName: Full=Phosphoribosylglycinamide synthetase;
GN   Name=purD; OrderedLocusNames=jhp_1140;
OS   Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=J99 / ATCC 700824;
RX   PubMed=9923682; DOI=10.1038/16495;
RA   Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C.,
RA   Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J.,
RA   Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D.,
RA   Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.;
RT   "Genomic sequence comparison of two unrelated isolates of the human gastric
RT   pathogen Helicobacter pylori.";
RL   Nature 397:176-180(1999).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-phospho-beta-D-ribosylamine + ATP + glycine = ADP + H(+) +
CC         N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate;
CC         Xref=Rhea:RHEA:17453, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:57305, ChEBI:CHEBI:58681,
CC         ChEBI:CHEBI:143788, ChEBI:CHEBI:456216; EC=6.3.4.13;
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-
CC       (5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-
CC       diphosphate: step 2/2.
CC   -!- SIMILARITY: Belongs to the GARS family. {ECO:0000305}.
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DR   EMBL; AE001439; AAD06709.1; -; Genomic_DNA.
DR   PIR; A71846; A71846.
DR   RefSeq; WP_000654427.1; NZ_CP011330.1.
DR   AlphaFoldDB; Q9ZK07; -.
DR   SMR; Q9ZK07; -.
DR   STRING; 85963.jhp_1140; -.
DR   EnsemblBacteria; AAD06709; AAD06709; jhp_1140.
DR   KEGG; hpj:jhp_1140; -.
DR   PATRIC; fig|85963.30.peg.1436; -.
DR   eggNOG; COG0151; Bacteria.
DR   OMA; QADNMPF; -.
DR   UniPathway; UPA00074; UER00125.
DR   Proteomes; UP000000804; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004637; F:phosphoribosylamine-glycine ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009113; P:purine nucleobase biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   Gene3D; 3.90.600.10; -; 1.
DR   HAMAP; MF_00138; GARS; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR016185; PreATP-grasp_dom_sf.
DR   InterPro; IPR020561; PRibGlycinamid_synth_ATP-grasp.
DR   InterPro; IPR000115; PRibGlycinamide_synth.
DR   InterPro; IPR020560; PRibGlycinamide_synth_C-dom.
DR   InterPro; IPR037123; PRibGlycinamide_synth_C_sf.
DR   InterPro; IPR020562; PRibGlycinamide_synth_N.
DR   InterPro; IPR011054; Rudment_hybrid_motif.
DR   PANTHER; PTHR43472; PTHR43472; 1.
DR   Pfam; PF01071; GARS_A; 1.
DR   Pfam; PF02843; GARS_C; 1.
DR   Pfam; PF02844; GARS_N; 1.
DR   SMART; SM01210; GARS_C; 1.
DR   SUPFAM; SSF51246; SSF51246; 1.
DR   SUPFAM; SSF52440; SSF52440; 1.
DR   TIGRFAMs; TIGR00877; purD; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; Purine biosynthesis.
FT   CHAIN           1..424
FT                   /note="Phosphoribosylamine--glycine ligase"
FT                   /id="PRO_0000151454"
FT   DOMAIN          111..312
FT                   /note="ATP-grasp"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00409"
FT   BINDING         137..189
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00409"
SQ   SEQUENCE   424 AA;  47511 MW;  BB4B8D38A4330836 CRC64;
     MKDNNNYNVL IVGNKGREYA LAQRLQQDER VNALYFCLGN GGTQDLGENL ECEHYEHIVE
     LALKKQIHLA IISEEEPLIL GLTEMLEKAG ILVFGASKEA AKLEASKSYM KAFVKECGIK
     SASYFETNDL KEALNYIQNA SFPLVIKALN KNTSIVHHQE EALKILEDAL KQSNEPVIIE
     PFLEGFELSV TALIANDDFI LLPFCQNYKR LLEGDNGVNT GGMGAIAPAN FFSNELEEKI
     KNHIFKPTLE KLQADNTPFK GVLLAEIVII EEKGVLEPYL LDFSVRFKDI ECQTILPLVE
     NPLLDLFLAT AKGELHSLEL VFSKEFVMSV ALVSRNYPTS SSPKQTLYID PVDEKKGHLI
     LGEVEQDNGV FESSGGRVIF AIGRGKSLLE ARNHAYEIAQ KVHFEGMFYR KDIGFKVLDL
     KEYS