ID   PUR2_VIGUN              Reviewed;         311 AA.
AC   P52421;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Phosphoribosylamine--glycine ligase;
DE            EC=6.3.4.13;
DE   AltName: Full=Glycinamide ribonucleotide synthetase;
DE            Short=GARS;
DE   AltName: Full=Phosphoribosylglycinamide synthetase;
DE   Flags: Fragment;
GN   Name=PUR2;
OS   Vigna unguiculata (Cowpea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Vigna.
OX   NCBI_TaxID=3917;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Vita 3; TISSUE=Root nodule;
RA   Smith P.M.C., Mann A.J., Hall D.J., Atkins C.A.;
RL   Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-phospho-beta-D-ribosylamine + ATP + glycine = ADP + H(+) +
CC         N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate;
CC         Xref=Rhea:RHEA:17453, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:57305, ChEBI:CHEBI:58681,
CC         ChEBI:CHEBI:143788, ChEBI:CHEBI:456216; EC=6.3.4.13;
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-
CC       (5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-
CC       diphosphate: step 2/2.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the GARS family. {ECO:0000305}.
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DR   EMBL; U30896; AAA74447.1; -; mRNA.
DR   PIR; T11532; T11532.
DR   AlphaFoldDB; P52421; -.
DR   SMR; P52421; -.
DR   UniPathway; UPA00074; UER00125.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004637; F:phosphoribosylamine-glycine ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009113; P:purine nucleobase biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   Gene3D; 3.90.600.10; -; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR020561; PRibGlycinamid_synth_ATP-grasp.
DR   InterPro; IPR000115; PRibGlycinamide_synth.
DR   InterPro; IPR020560; PRibGlycinamide_synth_C-dom.
DR   InterPro; IPR037123; PRibGlycinamide_synth_C_sf.
DR   InterPro; IPR020559; PRibGlycinamide_synth_CS.
DR   InterPro; IPR011054; Rudment_hybrid_motif.
DR   PANTHER; PTHR43472; PTHR43472; 1.
DR   Pfam; PF01071; GARS_A; 1.
DR   Pfam; PF02843; GARS_C; 1.
DR   SMART; SM01210; GARS_C; 1.
DR   SUPFAM; SSF51246; SSF51246; 1.
DR   TIGRFAMs; TIGR00877; purD; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
DR   PROSITE; PS00184; GARS; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Chloroplast; Ligase; Nucleotide-binding; Plastid;
KW   Purine biosynthesis.
FT   CHAIN           <1..311
FT                   /note="Phosphoribosylamine--glycine ligase"
FT                   /id="PRO_0000151518"
FT   DOMAIN          <1..191
FT                   /note="ATP-grasp"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00409"
FT   NON_TER         1
SQ   SEQUENCE   311 AA;  33070 MW;  B0CA31A1E2FCB227 CRC64;
     DPRVRKQYIQ EQGAPIVIKA DGLAAGKGVT VAMTLEEAYK AVDSMLVQGD FGSAGCRVIV
     EEFLEGEEAS FFALVDGENA IPLESAQDHK RVGDGDTGPN TGGMGAYSPA PVLTKELQSI
     VMDSIIIPTV KGMSAEGSKF VGVLYAGLMI EKKSGMPKLI EYNVRFGDPE CQVLMVRLES
     DLVQVLLAAC RGELNGVSLK WSPGSAMVVV MASKGYPGSY QKGTVIENLE EAEAVAPGIK
     IFHAGTAFDS EGRFIATGGR VLGVTAKGND LEEACDRAYL AVENVNWPGG FLPSGYWLES
     STSETSMLER E