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PUR3_MYCTU
ID   PUR3_MYCTU              Reviewed;         215 AA.
AC   P9WHM5; F2GHX1; L0T6W9; P71554; Q7D921;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 40.
DE   RecName: Full=Phosphoribosylglycinamide formyltransferase {ECO:0000255|HAMAP-Rule:MF_01930};
DE            EC=2.1.2.2 {ECO:0000255|HAMAP-Rule:MF_01930};
DE   AltName: Full=5'-phosphoribosylglycinamide transformylase {ECO:0000255|HAMAP-Rule:MF_01930};
DE   AltName: Full=GAR transformylase {ECO:0000255|HAMAP-Rule:MF_01930};
DE            Short=GART {ECO:0000255|HAMAP-Rule:MF_01930};
GN   Name=purN {ECO:0000255|HAMAP-Rule:MF_01930}; OrderedLocusNames=Rv0956;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) OF 2-215 IN COMPLEX WITH SUBSTRATE
RP   ANALOGS AND MAGNESIUM IONS, AND SUBUNIT.
RX   PubMed=19394344; DOI=10.1016/j.jmb.2009.04.044;
RA   Zhang Z., Caradoc-Davies T.T., Dickson J.M., Baker E.N., Squire C.J.;
RT   "Structures of glycinamide ribonucleotide transformylase (PurN) from
RT   Mycobacterium tuberculosis reveal a novel dimer with relevance to drug
RT   discovery.";
RL   J. Mol. Biol. 389:722-733(2009).
CC   -!- FUNCTION: Catalyzes the transfer of a formyl group from 10-
CC       formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR),
CC       producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and
CC       tetrahydrofolate. {ECO:0000255|HAMAP-Rule:MF_01930}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-10-formyltetrahydrofolate + N(1)-(5-phospho-beta-D-
CC         ribosyl)glycinamide = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N(2)-
CC         formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide;
CC         Xref=Rhea:RHEA:15053, ChEBI:CHEBI:15378, ChEBI:CHEBI:57453,
CC         ChEBI:CHEBI:57454, ChEBI:CHEBI:143788, ChEBI:CHEBI:147286;
CC         EC=2.1.2.2; Evidence={ECO:0000255|HAMAP-Rule:MF_01930};
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-
CC       formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-
CC       ribosyl)glycinamide (10-formyl THF route): step 1/1.
CC       {ECO:0000255|HAMAP-Rule:MF_01930}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:19394344}.
CC   -!- SIMILARITY: Belongs to the GART family. {ECO:0000255|HAMAP-
CC       Rule:MF_01930}.
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DR   EMBL; AL123456; CCP43704.1; -; Genomic_DNA.
DR   PIR; B70717; B70717.
DR   RefSeq; NP_215471.1; NC_000962.3.
DR   RefSeq; WP_003898661.1; NZ_NVQJ01000001.1.
DR   PDB; 3DA8; X-ray; 1.30 A; A/B=2-215.
DR   PDB; 3DCJ; X-ray; 2.20 A; A/B=2-215.
DR   PDBsum; 3DA8; -.
DR   PDBsum; 3DCJ; -.
DR   AlphaFoldDB; P9WHM5; -.
DR   SMR; P9WHM5; -.
DR   STRING; 83332.Rv0956; -.
DR   PaxDb; P9WHM5; -.
DR   DNASU; 885407; -.
DR   GeneID; 885407; -.
DR   KEGG; mtu:Rv0956; -.
DR   TubercuList; Rv0956; -.
DR   eggNOG; COG0299; Bacteria.
DR   OMA; TGITIHY; -.
DR   PhylomeDB; P9WHM5; -.
DR   BRENDA; 2.1.2.2; 3445.
DR   UniPathway; UPA00074; UER00126.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0000287; F:magnesium ion binding; IDA:MTBBASE.
DR   GO; GO:0004644; F:phosphoribosylglycinamide formyltransferase activity; IBA:GO_Central.
DR   GO; GO:0006189; P:'de novo' IMP biosynthetic process; IBA:GO_Central.
DR   GO; GO:0046653; P:tetrahydrofolate metabolic process; IDA:MTBBASE.
DR   CDD; cd08645; FMT_core_GART; 1.
DR   HAMAP; MF_01930; PurN; 1.
DR   InterPro; IPR002376; Formyl_transf_N.
DR   InterPro; IPR036477; Formyl_transf_N_sf.
DR   InterPro; IPR004607; GART.
DR   Pfam; PF00551; Formyl_trans_N; 1.
DR   SUPFAM; SSF53328; SSF53328; 1.
DR   TIGRFAMs; TIGR00639; PurN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Magnesium; Metal-binding; Purine biosynthesis;
KW   Reference proteome; Transferase.
FT   CHAIN           1..215
FT                   /note="Phosphoribosylglycinamide formyltransferase"
FT                   /id="PRO_0000420698"
FT   ACT_SITE        118
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01930"
FT   BINDING         74
FT                   /ligand="(6S)-10-formyltetrahydrofolate"
FT                   /ligand_id="ChEBI:CHEBI:57454"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01930"
FT   BINDING         100..102
FT                   /ligand="(6S)-10-formyltetrahydrofolate"
FT                   /ligand_id="ChEBI:CHEBI:57454"
FT   BINDING         116
FT                   /ligand="(6S)-10-formyltetrahydrofolate"
FT                   /ligand_id="ChEBI:CHEBI:57454"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01930"
FT   BINDING         116
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   BINDING         117
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   BINDING         150..154
FT                   /ligand="(6S)-10-formyltetrahydrofolate"
FT                   /ligand_id="ChEBI:CHEBI:57454"
FT   SITE            154
FT                   /note="Raises pKa of active site His"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01930"
FT   STRAND          5..7
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   STRAND          11..21
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   HELIX           24..32
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   STRAND          38..48
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   HELIX           51..58
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   STRAND          63..65
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   HELIX           68..70
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   STRAND          71..73
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   HELIX           74..86
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   STRAND          91..97
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   HELIX           104..110
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   TURN            111..113
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   STRAND          114..120
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   HELIX           130..137
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   STRAND          140..148
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   STRAND          151..154
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   STRAND          158..165
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   HELIX           172..197
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   STRAND          199..202
FT                   /evidence="ECO:0007829|PDB:3DA8"
FT   STRAND          205..208
FT                   /evidence="ECO:0007829|PDB:3DA8"
SQ   SEQUENCE   215 AA;  22416 MW;  55556C033097EAA1 CRC64;
     MQEPLRVPPS APARLVVLAS GTGSLLRSLL DAAVGDYPAR VVAVGVDREC RAAEIAAEAS
     VPVFTVRLAD HPSRDAWDVA ITAATAAHEP DLVVSAGFMR ILGPQFLSRF YGRTLNTHPA
     LLPAFPGTHG VADALAYGVK VTGATVHLVD AGTDTGPILA QQPVPVLDGD DEETLHERIK
     VTERRLLVAA VAALATHGVT VVGRTATMGR KVTIG
 
 
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