ID   PUR3_VIGUN              Reviewed;         312 AA.
AC   P52423; Q9XGS3;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   08-DEC-2000, sequence version 2.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Phosphoribosylglycinamide formyltransferase, chloroplastic;
DE            EC=2.1.2.2;
DE   AltName: Full=5'-phosphoribosylglycinamide transformylase;
DE   AltName: Full=GAR transformylase;
DE            Short=GART;
DE   Flags: Precursor;
GN   Name=PUR3;
OS   Vigna unguiculata (Cowpea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Vigna.
OX   NCBI_TaxID=3917;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. Vita 3; TISSUE=Root nodule;
RA   Hall D.J., Bussell J.D., Mann A.J., Goggin D.E., Atkins C.A., Smith P.M.C.;
RT   "Vupur3 mRNA from cowpea nodules encoding glycinamide ribonucleotide
RT   transformylase.";
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-10-formyltetrahydrofolate + N(1)-(5-phospho-beta-D-
CC         ribosyl)glycinamide = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N(2)-
CC         formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide;
CC         Xref=Rhea:RHEA:15053, ChEBI:CHEBI:15378, ChEBI:CHEBI:57453,
CC         ChEBI:CHEBI:57454, ChEBI:CHEBI:143788, ChEBI:CHEBI:147286;
CC         EC=2.1.2.2;
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-
CC       formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-
CC       ribosyl)glycinamide (10-formyl THF route): step 1/1.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the GART family. {ECO:0000305}.
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DR   EMBL; U30875; AAA75367.2; -; mRNA.
DR   EMBL; AF160196; AAD45353.2; -; Genomic_DNA.
DR   EMBL; AY189137; AAO25114.1; -; mRNA.
DR   EMBL; AY189138; AAO25115.1; -; mRNA.
DR   PIR; T11574; T11574.
DR   AlphaFoldDB; P52423; -.
DR   SMR; P52423; -.
DR   UniPathway; UPA00074; UER00126.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0004644; F:phosphoribosylglycinamide formyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd08645; FMT_core_GART; 1.
DR   HAMAP; MF_01930; PurN; 1.
DR   InterPro; IPR002376; Formyl_transf_N.
DR   InterPro; IPR036477; Formyl_transf_N_sf.
DR   InterPro; IPR004607; GART.
DR   InterPro; IPR001555; GART_AS.
DR   Pfam; PF00551; Formyl_trans_N; 1.
DR   SUPFAM; SSF53328; SSF53328; 1.
DR   TIGRFAMs; TIGR00639; PurN; 1.
DR   PROSITE; PS00373; GART; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Plastid; Purine biosynthesis; Transferase; Transit peptide.
FT   TRANSIT         1..73
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           74..312
FT                   /note="Phosphoribosylglycinamide formyltransferase,
FT                   chloroplastic"
FT                   /id="PRO_0000029878"
FT   ACT_SITE        206
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         109..111
FT                   /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide"
FT                   /ligand_id="ChEBI:CHEBI:143788"
FT                   /evidence="ECO:0000250"
FT   BINDING         162
FT                   /ligand="(6S)-10-formyltetrahydrofolate"
FT                   /ligand_id="ChEBI:CHEBI:57454"
FT                   /evidence="ECO:0000250"
FT   BINDING         187..190
FT                   /ligand="(6S)-10-formyltetrahydrofolate"
FT                   /ligand_id="ChEBI:CHEBI:57454"
FT                   /evidence="ECO:0000250"
FT   BINDING         204
FT                   /ligand="(6S)-10-formyltetrahydrofolate"
FT                   /ligand_id="ChEBI:CHEBI:57454"
FT                   /evidence="ECO:0000250"
FT   BINDING         247
FT                   /ligand="(6S)-10-formyltetrahydrofolate"
FT                   /ligand_id="ChEBI:CHEBI:57454"
FT                   /evidence="ECO:0000250"
FT   BINDING         276
FT                   /ligand="N(1)-(5-phospho-beta-D-ribosyl)glycinamide"
FT                   /ligand_id="ChEBI:CHEBI:143788"
FT                   /evidence="ECO:0000250"
FT   SITE            247
FT                   /note="Raises pKa of active site His"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   312 AA;  34583 MW;  51196B04E3D1385D CRC64;
     MEAQQIISRF CPKSSLAPSI PMVKQPFSLN FPPLHSLSSY PFLQSQNLGF PTGALHAISF
     VHKEVCSSSW RIWCSKSSSS TAEPEEDHEV RAQVTVRRKK LAVFVSGGGS NFRSIHEASK
     KGSLHGDVTV LVTNKSECGG AQYARNNGIP VILFPKAKDE PKGLSPCDLV DTLRKFEVDF
     VLLAGYLKLI PVELIRAFER SIFNIHPSLL PAFGGKGYYG MKVHKAVIAS GARFSGPTIH
     FVDEHYDTGR ILAQRVVPVL ANDTAEELAA RVLNEEHQLY VEVVEALCEE RIVWRKDGVP
     LIQSRENPNE FL