ID   AAD_PHACH               Reviewed;         385 AA.
AC   Q01752;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Aryl-alcohol dehydrogenase [NADP(+)];
DE            Short=AAD;
DE            EC=1.1.1.91;
OS   Phanerodontia chrysosporium (White-rot fungus) (Sporotrichum pruinosum).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Polyporales; Phanerochaetaceae; Phanerodontia.
OX   NCBI_TaxID=2822231;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=ATCC 24725 / DSM 6909 / CBS 481.73 / BCRC 36200 / NRRL 6361 / VKM
RC   F-1767;
RX   PubMed=7961751; DOI=10.1016/s0021-9258(18)46907-4;
RA   Reiser J., Muheim A., Hardegger M., Frank G., Fiechter A.;
RT   "Aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete
RT   chrysosporium. Gene cloning, sequence analysis, expression, and
RT   purification of the recombinant enzyme.";
RL   J. Biol. Chem. 269:28152-28159(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an aromatic primary alcohol + NADP(+) = an aromatic aldehyde +
CC         H(+) + NADPH; Xref=Rhea:RHEA:17761, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33855, ChEBI:CHEBI:33857, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349; EC=1.1.1.91;
CC   -!- PTM: The N-terminus is blocked.
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family. Aldo/keto
CC       reductase 2 subfamily. {ECO:0000305}.
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DR   EMBL; L08964; AAA61931.1; -; mRNA.
DR   PIR; A55449; A55449.
DR   AlphaFoldDB; Q01752; -.
DR   SMR; Q01752; -.
DR   VEuPathDB; FungiDB:AGR57_3931; -.
DR   OMA; CWERTED; -.
DR   GO; GO:0047681; F:aryl-alcohol dehydrogenase (NADP+) activity; IDA:SGD.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006081; P:cellular aldehyde metabolic process; IDA:SGD.
DR   Gene3D; 3.20.20.100; -; 1.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   SUPFAM; SSF51430; SSF51430; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Direct protein sequencing; NADP;
KW   Oxidoreductase.
FT   CHAIN           1..385
FT                   /note="Aryl-alcohol dehydrogenase [NADP(+)]"
FT                   /id="PRO_0000070364"
FT   ACT_SITE        76
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         238..248
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   385 AA;  43563 MW;  7D5D6E00690B9A20 CRC64;
     MNIWAPAPEP PTKLGRHRQL APGCGLHVSP IQLGAMSIGD KWHPYGMGTM DKEASFKLLD
     AFYNAGGNFI DTANVYQDET SEEFIGEWME ARGNRDQMVV ATKYSLVYKR GASFEEIPQK
     TQYVGNSLKS MHISVHDSLR KLRTSYIDIF YVHFWDYTCT IEEVMNGLHN LVAQGKVLYL
     GVSDTPAWVV SKANNYARMA GKTPFVIYEG EWNITMRDME RDIIPMCIHE GMAIAPWNVL
     CAGKIRTDAE EERRLKSGEG GRTLLQFDGW LRNETERKVS KALEKVAEEI GAKSITSVAI
     AYLMQKFPYV FPIVGGRKVE HLYANLEALD ISLSPEQMQF LNDTVPFNKG FPYLLFGDGS
     DYNIVHKAAG HYDKWPAQQA IRPQK