PUR6_CAEEL
ID PUR6_CAEEL Reviewed; 423 AA.
AC Q10457;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Probable multifunctional protein ADE2;
DE Includes:
DE RecName: Full=Phosphoribosylaminoimidazole-succinocarboxamide synthase;
DE EC=6.3.2.6;
DE AltName: Full=SAICAR synthetase;
DE Includes:
DE RecName: Full=Phosphoribosylaminoimidazole carboxylase;
DE EC=4.1.1.21;
DE AltName: Full=AIR carboxylase;
DE Short=AIRC;
GN Name=pacs-1 {ECO:0000312|WormBase:B0286.3};
GN ORFNames=B0286.3 {ECO:0000312|WormBase:B0286.3};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP +
CC L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-
CC 4-carboxamido]succinate + ADP + 2 H(+) + phosphate;
CC Xref=Rhea:RHEA:22628, ChEBI:CHEBI:15378, ChEBI:CHEBI:29991,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58443,
CC ChEBI:CHEBI:77657, ChEBI:CHEBI:456216; EC=6.3.2.6;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + H(+)
CC = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + CO2;
CC Xref=Rhea:RHEA:10792, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:77657, ChEBI:CHEBI:137981; EC=4.1.1.21;
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-
CC amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-
CC phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-
CC amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-
CC phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
CC -!- INTERACTION:
CC Q10457; Q10457: pacs-1; NbExp=2; IntAct=EBI-311886, EBI-311886;
CC -!- SIMILARITY: In the N-terminal section; belongs to the SAICAR synthetase
CC family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the AIR carboxylase
CC family. Class II subfamily. {ECO:0000305}.
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DR EMBL; BX284602; CCD61663.1; -; Genomic_DNA.
DR PIR; T15309; T15309.
DR RefSeq; NP_494771.1; NM_062370.5.
DR AlphaFoldDB; Q10457; -.
DR SMR; Q10457; -.
DR BioGRID; 39128; 43.
DR DIP; DIP-24510N; -.
DR IntAct; Q10457; 20.
DR STRING; 6239.B0286.3; -.
DR EPD; Q10457; -.
DR PaxDb; Q10457; -.
DR PeptideAtlas; Q10457; -.
DR EnsemblMetazoa; B0286.3.1; B0286.3.1; WBGene00015116.
DR GeneID; 173772; -.
DR KEGG; cel:CELE_B0286.3; -.
DR UCSC; B0286.3; c. elegans.
DR CTD; 173772; -.
DR WormBase; B0286.3; CE03863; WBGene00015116; pacs-1.
DR eggNOG; KOG2835; Eukaryota.
DR GeneTree; ENSGT00390000010172; -.
DR HOGENOM; CLU_061495_1_0_1; -.
DR InParanoid; Q10457; -.
DR OMA; WSDEQII; -.
DR OrthoDB; 566769at2759; -.
DR PhylomeDB; Q10457; -.
DR Reactome; R-CEL-73817; Purine ribonucleoside monophosphate biosynthesis.
DR SignaLink; Q10457; -.
DR UniPathway; UPA00074; UER00130.
DR UniPathway; UPA00074; UER00131.
DR PRO; PR:Q10457; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00015116; Expressed in larva and 4 other tissues.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0043727; F:5-amino-4-imidazole carboxylate lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0004638; F:phosphoribosylaminoimidazole carboxylase activity; IEA:UniProtKB-EC.
DR GO; GO:0004639; F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; IBA:GO_Central.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IBA:GO_Central.
DR HAMAP; MF_02045; PurE_classII; 1.
DR HAMAP; MF_00137; SAICAR_synth; 1.
DR InterPro; IPR033626; PurE_classII.
DR InterPro; IPR000031; PurE_dom.
DR InterPro; IPR028923; SAICAR_synt/ADE2_N.
DR InterPro; IPR018236; SAICAR_synthetase_CS.
DR Pfam; PF00731; AIRC; 1.
DR Pfam; PF01259; SAICAR_synt; 1.
DR SMART; SM01001; AIRC; 1.
DR TIGRFAMs; TIGR01162; purE; 1.
DR PROSITE; PS01057; SAICAR_SYNTHETASE_1; 1.
DR PROSITE; PS01058; SAICAR_SYNTHETASE_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Decarboxylase; Ligase; Lyase; Multifunctional enzyme;
KW Nucleotide-binding; Purine biosynthesis; Reference proteome.
FT CHAIN 1..423
FT /note="Probable multifunctional protein ADE2"
FT /id="PRO_0000075034"
FT REGION 1..263
FT /note="SAICAR synthetase"
FT REGION 264..423
FT /note="AIR carboxylase"
SQ SEQUENCE 423 AA; 46978 MW; 101CD65318C4804A CRC64;
MSSLAEIASR PENLELLAEG KTKQIFDIKG EKDYVLIRSK DSLTAFNAVR KNELEGKSRI
ASKTTSNVFE YLQLLGLPTH FEKSISETEF VARKCTMIPI EWVARRVATG SFLKRNPGVK
EGFRFNDLKL ETFFKDDAND DPQWTDEQIV SNGLMIDHLK IGREEISLMK KMTKLVFRAL
EKGWALSNSA LIDMKIEFGV TVEGEILLAD VIDNDSWRVW PENDRRLQLD KQVYRDMKEV
TEEGLALVLK NYTKVMDITA TFSKHQQKCH VLVIMGSGSD GVFARKISDE AKKFGLETTL
KVSSAHKTTS DTLEVIADFE ESGVPTVVIA VAGRSNGLGP VIAGNSSLPV INCPPPSEST
SLDIWSSLRM PNGIGCTTVL DPSEAALAAA KILASHNHIV FGKVLTAQLK NQINIYNANR
KLE
