PUR6_CANAL
ID PUR6_CANAL Reviewed; 568 AA.
AC Q92210; A0A1D8PK45; P78597; Q5ANJ5;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2011, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Phosphoribosylaminoimidazole carboxylase;
DE EC=4.1.1.21;
DE AltName: Full=AIR carboxylase;
DE Short=AIRC;
GN Name=ADE2; OrderedLocusNames=CAALFM_C304520CA;
GN ORFNames=CaO19.13327, CaO19.5906;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 32354 / B311;
RX PubMed=9219341;
RX DOI=10.1002/(sici)1097-0061(19970630)13:8<769::aid-yea133>3.0.co;2-p;
RA Schmuke J.J., Davisson V.J., Bonar S.L., Gheesling-Mullis K., Dotson S.B.;
RT "Sequence analysis of the Candida albicans ADE2 gene and physical
RT separation of the two functionally distinct domains of the
RT phosphoribosylaminoimidazole carboxylase.";
RL Yeast 13:769-776(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=9200816;
RX DOI=10.1002/(sici)1097-0061(19970615)13:7<673::aid-yea122>3.0.co;2-g;
RA Tsang W.K.P., Cao B.-Y., Wang J.;
RT "Sequence analysis of Candida albicans phosphoribosyl-aminoimidazole
RT carboxylase (ADE2) gene.";
RL Yeast 13:673-676(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + H(+)
CC = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + CO2;
CC Xref=Rhea:RHEA:10792, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:77657, ChEBI:CHEBI:137981; EC=4.1.1.21;
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-
CC amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-
CC phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
CC -!- SIMILARITY: In the C-terminal section; belongs to the AIR carboxylase
CC family. Class I subfamily. {ECO:0000305}.
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DR EMBL; U69606; AAC49755.1; -; Genomic_DNA.
DR EMBL; U75582; AAC49742.1; -; Genomic_DNA.
DR EMBL; CP017625; AOW28470.1; -; Genomic_DNA.
DR RefSeq; XP_723120.1; XM_718027.1.
DR AlphaFoldDB; Q92210; -.
DR SMR; Q92210; -.
DR BioGRID; 1218353; 1.
DR STRING; 237561.Q92210; -.
DR PRIDE; Q92210; -.
DR GeneID; 3635259; -.
DR KEGG; cal:CAALFM_C304520CA; -.
DR CGD; CAL0000185210; ADE2.
DR VEuPathDB; FungiDB:C3_04520C_A; -.
DR eggNOG; KOG2835; Eukaryota.
DR HOGENOM; CLU_011534_2_1_1; -.
DR InParanoid; Q92210; -.
DR OMA; CRAGRKM; -.
DR OrthoDB; 945274at2759; -.
DR BRENDA; 4.1.1.21; 1096.
DR UniPathway; UPA00074; UER00130.
DR PHI-base; PHI:196; -.
DR PRO; PR:Q92210; -.
DR Proteomes; UP000000559; Chromosome 3.
DR GO; GO:0043727; F:5-amino-4-imidazole carboxylate lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR GO; GO:0004638; F:phosphoribosylaminoimidazole carboxylase activity; IMP:CGD.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0046084; P:adenine biosynthetic process; IMP:CGD.
DR Gene3D; 3.30.1490.20; -; 1.
DR HAMAP; MF_01929; PurE_classI; 1.
DR HAMAP; MF_01928; PurK; 1.
DR InterPro; IPR016301; Ade2_fungi/plant.
DR InterPro; IPR011761; ATP-grasp.
DR InterPro; IPR003135; ATP-grasp_carboxylate-amine.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR016185; PreATP-grasp_dom_sf.
DR InterPro; IPR033747; PurE_ClassI.
DR InterPro; IPR000031; PurE_dom.
DR InterPro; IPR005875; PurK.
DR InterPro; IPR040686; PurK_C.
DR InterPro; IPR011054; Rudment_hybrid_motif.
DR Pfam; PF00731; AIRC; 1.
DR Pfam; PF02222; ATP-grasp; 1.
DR Pfam; PF17769; PurK_C; 1.
DR PIRSF; PIRSF001340; AIR_carboxylase; 1.
DR SMART; SM01001; AIRC; 1.
DR SUPFAM; SSF51246; SSF51246; 1.
DR SUPFAM; SSF52440; SSF52440; 1.
DR TIGRFAMs; TIGR01162; purE; 1.
DR TIGRFAMs; TIGR01161; purK; 1.
DR PROSITE; PS50975; ATP_GRASP; 1.
PE 3: Inferred from homology;
KW ATP-binding; Decarboxylase; Lyase; Nucleotide-binding; Purine biosynthesis;
KW Reference proteome.
FT CHAIN 1..568
FT /note="Phosphoribosylaminoimidazole carboxylase"
FT /id="PRO_0000075021"
FT DOMAIN 110..298
FT /note="ATP-grasp"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00409"
FT BINDING 138..193
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00409"
FT VARIANT 219
FT /note="C -> W (in strain: ATCC 32354 / B311)"
FT VARIANT 248
FT /note="L -> P (in strain: ATCC 32354 / B311)"
FT VARIANT 332
FT /note="L -> S (in strain: ATCC 32354 / B311)"
FT VARIANT 352
FT /note="T -> A (in strain: ATCC 32354 / B311)"
FT CONFLICT 233..234
FT /note="AK -> GQ (in Ref. 1; AAC49755 and 2; AAC49742)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 568 AA; 62413 MW; AEB63A6E0F193F44 CRC64;
MDSKTVGILG GGQLGRMIVE AAHRLNIKTV ILDAAKSPAK QINALDDHVD GSFTNYDSIV
KLAEKADVLT VEIEHVDVDA LIKVQEKFPK VEIYPLPETI RLIQDKYLQK NHLIKHDVAV
TESVAVETNT VDDLLHIGEK FGYPYMLKSR TLAYDGRGNF VVKDKSYCEK ALEFLKDRPL
YAEKWCPFTK ELAVMVVRSL EGEVFAYPTV ETIHENNICH LVYAPARIPD TLAKKASILA
KNAVKSFLGC GIFGVEMFLL ENNELLINEI APRPHNSGHY TIDACVTSQF EAHVRAVTGL
PMPKGFTEFS TSITNAIMLN VLGDKATPNK ELEICRRALE TPHASVYLYG KTTRPERKMG
HINVVTSSMQ DAESRLSYIL GDTTEIPKSL ATDKESPLVG IIMGSDSDLP VMAVGARILK
QFGVPFELTI VSAHRTPHRM SEYAIEAPKR GLKCIIAGAG GAAHLPGMVA AMTPLPVIGV
PVKGSTLDGV DSLHSIVQMP RGIPVATVAI NNSTNAALLA IRILGAYDSK WLTEMNQYML
NMETEVLGKA ETLEEIGYED YLTDKLKK
